bs-0756R [Primary Antibody]
Vimentin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Vimentin

Specificity: KO-Validated

Immunogen Range: 371-466/466


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7431

Swiss Prot: P08670

Source: KLH conjugated synthetic peptide derived from human Vimentin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat
Bovine
Pig
Others
Monkey

Predicted Cross Reactive Species: Cow
Chicken
Goat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Yueqin, et al. "Hypoxia-Inducible Factor-1alpha and MAPK Co-Regulate Activation of Hepatic Stellate Cells upon Hypoxia Stimulation." PLoS ONE 8.9 (2013).Read more>>
  • Hu, Bin, et al. "IFN‐γ Inhibits Osteopontin Expression in Human Decidual Stromal Cells and can be Attenuated by 1α, 25‐Dihydroxyvitamin D3." American Journal of Reproductive Immunology 68.4 (2012): 353-361.Read more>>
  • Liu, Yang, et al. "Down-regulation of< i> Wnt10a</i> affects odontogenesis and proliferation in mesenchymal cells." Biochemical and Biophysical Research Communications (2013).Read more>>
  • Fu, Min, et al. "Effects of Ureaplasma urealyticum lipid-associated membrane proteins on rheumatoid arthritis synovial fibroblasts." Journal of International Medical Research 41.5 (2013): 1655-1670.Read more>>
  • Li, Yingli, et al. "Differences between Niche Cells and Limbal Stromal Cells in Maintenance of Corneal Limbal Stem Cells." Investigative Ophthalmology & Visual Science (2014): IOVS-13Read more>>
  • Sottnik, Joseph L., et al. "Osteocytes Serve as a Progenitor Cell of Osteosarcoma." Journal of Cellular Biochemistry (2014).Read more>>
  • Wang, Jing, et al. "Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection." Journal of Virological Methods (2014).Read more>>
  • Pan, Yan, et al. "Enoxaparin Sensitizes Human Non-small-cell Lung Carcinomas to Gefitinib by Inhibiting DOCK1 Expression, Vimentin Phosphorylation and Akt Activation." Molecular pharmacology (2014): mol-114.Read more>>
  • Tong, Weihua, et al. "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." Cell Biochemistry and Biophysics (2015): 1-7.Read more>>
  • Cheng, Gangwei, et al. "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." Cell Biochemistry and Biophysics: 1-6.Read more>>
  • Deng, Boya, et al. "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." Tumor Biology (2015): 1-9.Read more>>
  • Fernández-Ferreiro, Anxo, et al. "In vitro and in vivo ocular safety and eye surface permanence determination by direct and Magnetic Resonance Imaging of ion-sensitive hydrogels based on Gellan gum and kappa-carrageenan."European Journal of Pharmaceutics and Biopharmaceutics (2015).Read more>>
  • Karim, A. S., et al. "Nox2 Is a Mediator of Ischemia Reperfusion Injury."American Journal of Transplantation (2015).Read more>>
  • Wang, Li-Ping, et al. "Angiotensin II upregulates K Ca 3.1 channels and stimulates cell proliferation in rat cardiac fibroblasts." Biochemical pharmacology 85.10 (2013): 1486-1494.Read more>>
  • Su, Jing, and Hong Li. "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." International Journal of Clinical and Experimental Pathology 8.9 (2015): 10760-10767.Read more>>
  • Huang, Chao, and Bin Wen. "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." Molecular Biology Reports: 1-10.Read more>>
  • Sucre, Jennifer MS, et al. "A three-dimensional human model of the fibroblast activation that accompanies bronchopulmonary dysplasia identifies Notch-mediated pathophysiology." American Journal of Physiology-Lung Cellular and Molecular Physiology 310.10 (2016): L889-L898.Read more>>
  • Chen, Jia, et al. "Biological characterization of metanephric mesenchymal stem cells from the Beijing duck." Experimental and Therapeutic Medicine 11.2 (2016): 439-447.Read more>>
  • Wilkinson, Dan C., et al. "Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling." Stem Cells Translational Medicine (2016): sctm-2016.Read more>>
  • Yap, Chan Choo, et al. "Different doublecortin (DCX) patient alleles show distinct phenotypes in cultured neurons: evidence for divergent loss-of-function and off-pathway cellular mechanisms." Journal of Biological Chemistry (2016): jbc-M116.Read more>>
  • Li, Junqin, et al. "Fatty acid synthase mediates the epithelial-mesenchymal transition of breast cancer cells." International Journal of Biological Sciences10.2 (2014): 171.Read more>>
  • Pagani, Isabel, et al. "Human Endometrial Stromal Cells Are Highly Permissive To Productive Infection by Zika Virus." bioRxiv (2016): 077305.Read more>>
  • Xiao, Jiajia, et al. "Isolation of Bovine Skin-Derived Precursor Cells and Their Developmental Potential After Nuclear Transfer." Cellular Reprogramming (Formerly" Cloning and Stem Cells") 18.6 (2016): 411-418.Read more>>
  • Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103.Read more>>
  • Smith, Elizabeth E., et al. "Developing a biomimetic tooth bud model." Journal of Tissue Engineering and Regenerative Medicine (2017).Read more>>
  • Khuder, Saja S. Regulation of Expression of CEACAM1 and Functional Correlation in Metabolic Diseases. Diss. University of Toledo, 2016.Read more>>
  • Huang, Chao, and Bin Wen. "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." Molecular Biology Reports 43.10 (2016): 1069-1078.Read more>>
  • Wang, Jing, et al. "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." BMC biology 15.1 (2017): 10.Read more>>
  • Tanos, T., et al. "Isolation of putative stem cells present in human adult olfactory mucosa." PloS one 12.7 (2017): e0181151.Read more>>
  • Park, Song, et al. "Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency, proliferation, and differentiation." Cell Biochemistry and Function (2017).Read more>>
  • Pan et al. Roles of vimentin and 14-3-3 zeta/delta in the inhibitory effects of heparin on PC-3M cell proliferation and B16-F10-luc-G5 cells metastasis. (2012) Acta.Pharmacol.Si. 33:798-808Read more>>
  • Gao et al. Hes1 promotes cell proliferation and migration by activating Bmi-1 and PTEN/Akt/GSK3β pathway in human colon cancer. (2015) Oncotarge. 6:38667-80Read more>>
  • Wang et al. 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts. (2017) Med.Sci.Moni. 23:46-56Read more>>
  • Chan et al. Investigating the Radioresistant Properties of Lung Cancer Stem Cells in the Context of the Tumor Microenvironment. (2016) Radiat.Res. 185:169-81Read more>>
  • Djamali et al. Nox2 and Cyclosporine-Induced Renal Hypoxia. (2016) Transplantation. 100:1198-210Read more>>
  • Fagundes et al. Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats. (2016) Oxid.Med.Cell.Longe. 2016:7323627Read more>>
  • Yap et al. Different Doublecortin (DCX) Patient Alleles Show Distinct Phenotypes in Cultured Neurons: EVIDENCE FOR DIVERGENT LOSS-OF-FUNCTION AND "OFF-PATHWAY" CELLULAR MECHANISMS. (2016) J.Biol.Chem. 291:26613-26626Read more>>
  • Khayat et al. GelMA-Encapsulated hDPSCs and HUVECs for Dental Pulp Regeneration. (2017) J.Dent.Res. 96:192-199Read more>>
  • Wang et al. The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma. (2017) J.Cell.Sci. 130:1078-1093Read more>>
  • Wang et al. Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene. (2017) PLoS.One. 12:e0172233Read more>>
  • Pagani et al. Human Endometrial Stromal Cells Are Highly Permissive To Productive Infection by Zika Virus. (2017) Sci.Rep. 7:44286Read more>>
  • Zhou et al. GDC-0449 improves the antitumor activity of nano-doxorubicin in pancreatic cancer in a fibroblast-enriched microenvironment. (2017) Sci.Rep. 7:13379Read more>>
  • Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49Read more>>
  • Zhou et al. Induced Pluripotent Stem Cells Inhibit Bleomycin-Induced Pulmonary Fibrosis in Mice through Suppressing TGF-β1/Smad-Mediated Epithelial to Mesenchymal Transition. (2016) Front.Pharmacol. 7:430Read more>>
  • Shan et al. Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells. (2016) Int.J.Clin.Exp.Pathol. 8:12357-67Read more>>
  • Fei et al. The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. (2015) J.Exp.Clin.Cancer.Res. 34:158Read more>>
  • Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543Read more>>
  • Monteiro et al. Dental cell sheet biomimetic tooth bud model. (2016) Biomaterials. 106:167-79Read more>>
  • Wang et al. Expression and clinical significance of autophagic protein LC3B and EMT markers in gastric cancer. (2018) Cancer.Manag.Res. 10:1479-1486Read more>>
  • Tarique I et al. LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle. Reprod Biol Endocrinol. 2019 Feb 9;17(1):19. Read more>>
VALIDATION IMAGES

Image submitted by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining and detection.


Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300, followed by conjugation to the secondary antibody and DAB staining


Human Jurkat cells probed with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


This image was kindly submitted by Preethi Vijayaraj from UCLA; 4% PFA fixed cells, permeabilized with 0.1% Triton/TBS for 10 minutes, blocked with proteinblock (DAKO) for 30 minutes, stained with 1:100 primary antibody for 1 hour at room temperature followed by 1:200 AlexaFluor 488 secondary antibody for 45 minutes


Image provided by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 dilution.


20 μg proteins of cell lysates from U138, HeLa and A431 were separated by SDS-PAGE and transferred to PVDF membrane for western blotting analysis antibodies against protein VIM at dilution of 1:1000 with exposure time of 10 seconds.


Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT


Lane 1: Hela cell lysates; Lane 2: Hela KO Vimentin cell lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


HeLa cells were stained with Vimentin Polyclonal Antibody, Unconjugated(bs-0756R) at 1:50 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


U-2 OS cells were stained with Vimentin Polyclonal Antibody, Unconjugated(bs-0756R) at 1:50 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


U251 cells were stained with Vimentin Polyclonal Antibody, Unconjugated(bs-0756R) at 1:50 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Vimentin Polyclonal Antibody(bs-0756R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Vimentin Polyclonal Antibody, conjugated (bs-0756R-AF488) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: U-87 MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Vimentin Polyclonal Antibody, conjugated (bs-0756R-AF488) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Vimentin Polyclonal Antibody, conjugated (bs-0756R-AF488) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Vimentin Polyclonal Antibody, conjugated (bs-0756R-AF555) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Vimentin Polyclonal Antibody, conjugated (bs-0756R-AF594) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: U-87 MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Vimentin Polyclonal Antibody, conjugated (bs-0756R-AF594) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37°C for 20 min; Incubation: Anti-Vimentin Polyclonal Antibody, conjugated (bs-0756R-AF594) 1:50, 2 hours at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human SH-SY5Y cell lysates; Lane 5: Human A549 cell lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C


Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human tonsil ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrium Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Vimentin polyclonal antibody, unconjugated (bs-0756R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.