bs-0754R [Primary Antibody]
PCNA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PCNA

Immunogen Range: 185-261/261


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5111

Swiss Prot: P12004

Source: KLH conjugated synthetic peptide derived from human PCNA

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464.Read more>>
  • Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).Read more>>
  • Sun, Y‐L., et al. "Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows." Reproduction in domestic animals 47.4 (2012): 601-608.Read more>>
  • Yin, H., et al. "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." Oncotarget (2016).Read more>>
  • Bi et al. Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats. (2015) Exp.Ther.Me. 10:851-856Read more>>
  • Li et al. Urotensin II promotes atherosclerosis in cholesterol-fed rabbits. (2014) PLoS.On. 9:e95089Read more>>
  • Li et al. Urotensin II promotes atherosclerosis in cholesterol-fed rabbits. (2014) PLoS.On. 9:e95089Read more>>
  • Hu et al. The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B1. (2018) Sci.Rep. 8:1770Read more>>
  • He et al. Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2. (2018) Oncol.Lett. 15:3977-3984Read more>>
  • Zhang et al. Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice. (2015) World.J.Gastroenterol. 21:4195-209Read more>>
  • Zhang et al. Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes. (2018) Molecules. 23 Read more>>
  • Li Q et al. All-trans retinoic acid regulates sheep primary myoblasts proliferation and differentiation in vitro. Domestic Animal Endocrinology,2019 106394. Read more>>
  • Shao F et al. Multiboosting of cancer immunotherapy by a core-shell delivery system. Mol Pharm. 2020 Jan 6;17(1):338-348.Read more>>
  • Zhang J et al. Down‐regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. J Cell Mol Med. 2019 Nov 17. Read more>>
  • Hua CD et al. Repression of Dok7 expression mediated by DNMT1 promotes glioma cells proliferation.Biomed Pharmacother. 2018 Oct;106:678-685. Read more>>
  • Ting Zhang. et al. Sea buckthorn ( Hippophae rhamnoides L.) oil enhances proliferation, adipocytes differentiation and insulin sensitivity in 3T3-L1 cells. Food Sci Biotechnol. 2020 Nov;29(11):1511-1518Read more>>
  • Hongzhong Cheng. et al. Circular RNA circLPAR3 Facilitates Esophageal Squamous Cell Carcinoma Progression Through Upregulating HMGB1 via Sponging miR-375/miR-433. Oncotargets Ther. 2020; 13: 7759C7771Read more>>
  • Xue Zhang. et al. Stomatin-like protein 2 (SLP2) regulates the proliferation and invasion of trophoblast cells by modulating mitochondrial functions. Placenta. 2020 Oct;100:13Read more>>
  • Jiajun Yang. et al. Selenium enriched Bacillus subtilis yb-1114246 activated the TLR2CNF-B1 signaling pathway to regulate chicken intestinal -defensin 1 expression. 2021 May 18Read more>>
  • Enoch Appiah Adu-Gyamfi. et al. Iodothyronine deiodinase 2 (DiO2) regulates trophoblast cell line cycle, invasion and apoptosis; and its downregulation is associated with early recurrent miscarriage. Placenta. 2021 Aug;111:54Read more>>
  • Wen Shi. et al. METTL3 Promotes Activation and Inflammation of FLSs Through the NF-B Signaling Pathway in Rheumatoid Arthritis. Front Med-Lausanne. 2021; 8: 607585Read more>>
  • Zi-Wei Wang. et al. Ex Vivo and In Vitro Studies Revealed Underlying Mechanisms of Immature Intestinal Inflammatory Responses Caused by Aflatoxin M1 Together with Ochratoxin A. Toxins. 2022 Mar;14(3):173Read more>>
  • Mengmeng Zhang. et al. Engineering a curcumol-loaded porphyrinic metal-organic framework for enhanced cancer photodynamic therapy. Colloid Surface B. 2022 Jun;214:112456Read more>>
  • Peng Xi. et al. The mechanism of dioscin preventing lung cancer based on network pharmacology and experimental validation. J Ethnopharmacol. 2022 Mar;:115138Read more>>
  • Tingting Bao. et al. Naringin prevents follicular atresia by inhibiting oxidative stress in the aging chicken. Poultry Sci. 2022 Mar;:101891Read more>>
  • Hanyue Li. et al. Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway. MOL METAB. 2022 Oct;:101617Read more>>
  • Shikuo Rong. et al. Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting TLR4/MyD88/NF-<i></i>B and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced BV2 Microglia. MEDIAT INFLAMM. 2022 Dec 06;2022:5184721Read more>>
  • Zhao Xuan. et al. CircSFMBT2 Plays an Oncogenic Role in Lung Adenocarcinoma Depending on the miR-1305/SALL4 Axis. BIOCHEM GENET. 2023 Dec;:1-19Read more>>
  • Zhao Jiamin. et al. Sea buckthorn oil regulates primary myoblasts proliferation and differentiation in vitro. IN VITRO CELL DEV-AN. 2023 Dec;:1-12Read more>>
  • Zerrin Barut. et al. Antiproliferative Effect of 7-Ketositosterol in Breast and Liver Cancer Cells: Possible Impact on Ceramide, Extracellular Signal-Regulated Kinases, and Nuclear Factor Kappa B Signaling Pathways. PHARMACEUTICALS-BASE. 2024 Jul;17(7):86Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat thymus tissue labeled Anti-PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


A549 lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Mouse NIH/3T3 cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Rat Large intestine lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1:Rat Large intestine lysates; Lane 2: Human Siha cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human HepG2 cell lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with PCNA polyclonal antibody, unconjugated (bs-0754R) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.