bs-0749R [Primary Antibody]
KISS1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KISS1

Specificity: This antibody detects Kisspeptin-10 region of Kisspeptin.

Immunogen Range: 81-145/145


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3814

Swiss Prot: Q15726

Source: KLH conjugated synthetic peptide derived from human KISS1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-1 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-1, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-1 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yun, Sung Gyu, et al. "The Effects of Different Wavelengths of Light-Emitting Diodes on the Expression of Reproduction-Related Genes in Goldfish Carassius auratus." Fisheries and Aquatic Sciences (2015).Read more>>
  • Liu et al. Di-(2-ethylhexyl) phthalate induces precocious puberty in adolescent female rats. (2018) Iran.J.Basic.Med.Sci. 21:848-855Read more>>
  • Meng-fan Peng. et al. Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet. J Ethnopharmacol. 2021 Jun;273:113947Read more>>
  • Lu Xiaosheng. et al. Deficiency of C1QL1 reduced murine ovarian follicle reserve through intraovarian and endocrine control. ENDOCRINOLOGY. 2022 ApRead more>>
  • Luz Patricia Muoz de la Torre. et al. Sex-specific hypothalamic expression of kisspeptin, gonadotropin releasing hormone, and kisspeptin receptor in progressive demyelination model. J CHEM NEUROANAT. 2022 Sep;123:10212Read more>>
  • Marilou Poitras. et al. Global cerebral ischemia in adult female rats interrupts estrous cyclicity and induces lasting changes in hypothalamic-pituitary-gonadal axis signaling peptides. NEUROSCI LETT. 2024 Jan;819:137578Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon cancer labeled with Rabbit Anti-Kiss 1/Kisspeptin Polyclonal Antibody (bs-0749R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded human colon cancer Rabbit Anti-Kiss 1/Kisspeptin Polyclonal Antibody (bs-0749R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin-embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Kisspeptin Polyclonal Antibody, Unconjugated (bs-0749R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.


Lane 1: Human HeLa cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human DU145 cell lysates probed with KISS1 Polyclonal Antibody, Unconjugated (bs-0749R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.