bs-0747R
[Primary Antibody]
RANKL Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: RANKL
Immunogen Range: 210-317/317
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8600
Swiss Prot: O14788
Source: KLH conjugated synthetic peptide derived from human RANKL
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 35
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Li, Ping. "Efficacy and Safety of Echinacoside in a Rat Osteopenia Model." Evidence-Based Complementary and Alternative Medicine 2013 (2013).Read more>>
- Sun, Jiabing, et al. "A Crucial Role of IL-17 in Bone Resorption During Rejection of Fresh Bone Xenotransplantation in Rats." Cell Biochemistry and Biophysics: 1-7.Read more>>
- Yu, Xijiao, et al. "Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats." Journal of Molecular Histology: 1-9.Read more>>
- Zhu, Wenjing, et al. "Effect of PI3K/Akt Signaling Pathway on the Process of Prostate Cancer Metastasis to Bone." Cell Biochemistry and Biophysics (2015): 1-7.Read more>>
- Ota, Takehiro, et al. "Expression of colony-stimulating factor 1 is associated with occurrence of osteochondral change in pigmented villonodular synovitis."Tumor Biology (2015): 1-7.Read more>>
- Li, Xianxian, et al. "Oral administration of 5-Hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats." Archives of Oral Biology(2015).Read more>>
- Zhang, Yuanyu, et al. "Mycobacterium tuberculosis 10-kDa co-chaperonin regulates the expression levels of receptor activator of nuclear factor-κB ligand and osteoprotegerin in human osteoblasts." Experimental and Therapeutic Medicine.Read more>>
- Chen, Zhiguang, et al. "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway."International Journal of Molecular Medicine.Read more>>
- Xiao, Wanan, et al. "Bone fracture healing is delayed in splenectomic rats." Life Sciences (2016).Read more>>
- Zhang, Xiaonan, et al. "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway." Chemico-Biological Interactions 256 (2016): 188-197.Read more>>
- Dolci, Gabriel Schmidt, et al. "Atorvastatin-induced osteoclast inhibition reduces orthodontic relapse." American Journal of Orthodontics and Dentofacial Orthopedics 151.3 (2017): 528-538.Read more>>
- He, Ming, et al. "Effect of glucocorticoids on osteoclast function in a mouse model of bone necrosis." Molecular medicine reports 14.2 (2016): 1054-1060.Read more>>
- Yu, X., et al. "Denervation effectively aggravates rat experimental periodontitis." Journal of Periodontal Research (2017).Read more>>
- Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).Read more>>
- Chen, Helin, et al. "Intermittent administration of parathyroid hormone ameliorated alveolar bone loss in experimental periodontitis in streptozotocin-induced diabetic rats." Archives of Oral Biology (2017).Read more>>
- Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546Read more>>
- Peng W et al.Expression of osteoprotegerin and receptor activator for the nuclear factor-κB ligand in XACB/LV-bFGF/MSCs transplantation for repair of rabbit femoral head defect necrosis.(2018) J. Cell. Biochem. Oct 18Read more>>
- Chen Y et al. Moxibustion of Zusanli (ST36) and Shenshu (BL23) Alleviates Cartilage Degradation through RANKL/OPG Signaling in a Rabbit Model of Rheumatoid Arthritis. Evid Based Complement Alternat Med. 2019 Jan 3;2019:6436420.Read more>>
- Yu X et al. Substance P aggravates periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019.Read more>>
- Yan KX et al. Substance P participates in periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019 Dec.Read more>>
- Zhang C et al. FOXO1 Mediates Advanced Glycation End Products Induced Mouse Osteocyte-Like MLO-Y4 Cell Apoptosis and Dysfunctions. Journal of Diabetes Research.2019, Article ID 6757428.Read more>>
- Kang J et al. Matrine protects retinal ganglion cells from apoptosis in experimental optic neuritis. 29 August 2019, PREPRINT (Version 1) available at Research Square.Read more>>
- Zou MX et al. Clinical Impact of the Immune Microenvironment in Spinal Chordoma: Immunoscore as an Independent Favorable Prognostic Factor.Neurosurgery. 2018 Jul 19. Read more>>
- Yang Y et al. Ganoderma lucidum Immune Modulator Protein rLZ-8 Could Prevent and Reverse Bone Loss in Glucocorticoids-Induced Osteoporosis Rat Model. Front Pharmacol. 2020 May 19;11:731.Read more>>
- Teng X et al. Effects of Low Dietary Phosphorus on Tibia Quality and Metabolism in Caged Laying Hens. Prev Vet Med. 2020 Aug;181:105049.Read more>>
- Daisuke Nishida. et al. RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp. Sci Rep-Uk. 2021 Feb;11(1):1-12Read more>>
- Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127Read more>>
- Yongkui Wang. et al. Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3/-catenin signaling. Toxicol Appl Pharm. 2020 Dec;409:115333Read more>>
- Yumi Sakamoto. et al. High-mobility group box 1 induces bone destruction associated with advanced oral squamous cancer via RAGE and TLR4. Biochem Bioph Res Co. 2020 Oct;531:422Read more>>
- Wang Tong. et al. Acupotomy Contributes to Suppressing Subchondral Bone Resorption in KOA Rabbits by Regulating the OPG/RANKL Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8168657Read more>>
- Yutao Cui. et al. Functionalized anti-osteoporosis drug delivery system enhances osseointegration of an inorganicCorganic bioactive interface in osteoporotic microenvironment. Mater Design. 2021 Aug;206:109753Read more>>
- aixiang Wang. et al. Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro. J Zhejiang Univ-Sc B. 2021 May;22(5):410-42Read more>>
- Shunichiro Matsuoka. et al. Undifferentiated Pleomorphic Sarcoma of Soft Tissue with Multinucleated Giant Cells with Osteogenic Phenotypes: A Mimicker of Malignant Giant Cell Tumor of Soft Tissue. 2021 Jul 21Read more>>
- Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094Read more>>
- Hiroki Ehara. et al. Role of tissue factor in delayed bone repair induced by diabetic state in mice. Plos One. 2021 Dec;16(12):e0260754Read more>>
- Qiusheng Shan. et al. Significance of cancer stroma for bone destruction in oral squamous cell carcinoma using different cancer stroma subtypes. Oncol Rep. 2022 Apr;47(4):1-13Read more>>
- Lingli Ding. et al. Ginsenoside Compound K Enhances Fracture Healing <i>via</i> Promoting Osteogenesis and Angiogenesis.. FRONT PHARMACOL. 2022 Apr;13:855393-855393Read more>>
- Huijuan Shen. et al. microRNA-146a mediates distraction osteogenesis via bone mesenchymal stem cell inflammatory response. ACTA HISTOCHEM. 2022 Aug;124:151913Read more>>
- Qiusheng Shan. et al. Investigation of bone invasion and underlying mechanisms of oral cancer using a cell line?derived xenograft model. ONCOL LETT. 2022 Nov;24(5):1-12Read more>>
- Albert chun-shuo Huang. et al. NF-κB Decoy ODN-Loaded Poly(Lactic-co-glycolic Acid) Nanospheres Inhibit Alveolar Ridge Resorption. INT J MOL SCI. 2023 Jan;24(4):3699Read more>>
- Yang Liu. et al. Isochlorogenic Acid C Restrains Erk/JNK/NF-B Signaling to Alleviate Inflammatory Response and Promote Cell Apoptosis. J FOOD BIOCHEM. 2023;2023:5547108Read more>>
- Xue Jiang. et al. Regulation of Wnt Signaling Pathway by Costic Acid Derivative, An Efficient Strategy for Treatment of Glucocorticoid-Induced Osteoporosis in Rat Model. CHEMISTRYSELECT. 2023 Apr;8(13):e202204912Read more>>
- Cheng Lanlan. et al. Liraglutide attenuates palmitate-induced apoptosis via PKA/-catenin/Bcl-2/Bax pathway in MC3T3-E1 cells. N-S ARCH PHARMACOL. 2023 Jul;:1-13Read more>>
- Hongxing Zheng. et al. Chromium Picolinate Regulates Bone Metabolism and Prevents Bone Loss in Diabetic Rats. MOLECULES. 2024 Jan;29(5):924Read more>>
- Rintanalert Duangtawan. et al. SDF-1 involvement in orthodontic tooth movement after tooth extraction. SCI REP-UK. 2024 Feb;14(1):1-15Read more>>
- Wang Qi. et al. Correlation between PD-1/PD-L1 and RANKL/OPG in chronic apical periodontitis model of Sprague-Dawley rats. ODONTOLOGY. 2024 Mar;:1-1Read more>>
- Wang Liu. et al. Yunnan Baiyao Might Mitigate Periodontitis Bone Destruction by Inhibiting Autophagy and Promoting Osteoblast Differentiation in vivo, ex vivo and in vitro. J INFLAMM RES. 2024 Apr 14Read more>>
- Erika Yamashita. et al. Effects of Improper Mechanical Force on the Production of Sonic Hedgehog, RANKL, and IL-6 in Human Periodontal Ligament Cells In Vitro. Dentistry Journal. 2024 Apr;12(4):108Read more>>
- Lihua Feng. et al. Maternal F-53B exposure during pregnancy and lactation affects bone growth and development in male offspring. ECOTOX ENVIRON SAFE. 2024 Jul;279:116501Read more>>
- Francesca Salamanna. et al. A Pilot Study on Circulating, Cellular, and Tissue Biomarkers in Osteosarcopenic Patients. INT J MOL SCI. 2024 Jan;25(11):5879Read more>>
VALIDATION IMAGES
Human raji cells probed with RANKL Polyclonal Antibody, Unconjugated (bs-0747R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated (bs-0747R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated (bs-0747R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
mouse splenocytes cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RANKL Polyclonal Antibody(bs-0747R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with RANKL'CD254 polyclonal antibody, unconjugated (bs-0747R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.