bs-0737R-BF488 [Conjugated Primary Antibody]
HIF-1 Alpha Polyclonal Antibody, AbBy Fluor® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HIF-1 Alpha

Immunogen Range: 341-450/826


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3091

Swiss Prot: Q16665

Source: KLH conjugated synthetic peptide derived from human of human HIF-1 Alpha

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 4 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP3. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

Conjugation: AbBy Fluor® 488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 92


Cross Reactive Species: Human
Mouse
Rat
Rabbit
Others

Predicted Cross Reactive Species: Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shou, Zhu, et al. "Expression and prognosis of FOXO3a and HIF-1?? in nasopharyngeal carcinoma."Journal of cancer research and clinical oncology 138.4 (2012): 585-593..Read more>>
  • Fan, Shengjun, et al. "Opposite angiogenic outcome of curcumin against ischemia and Lewis lung cancer models:in silico, in vitro and in vivo studies." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2014).Read more>>
  • Guo, Wei, et al. "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy." BMC Nephrology 15.1 (2014): 110.Read more>>
  • Zhang, Huimin, et al. "Vascular Normalization Induced by Sinomenine Hydrochloride Results in Suppressed Mammary Tumor Growth and Metastasis." Scientific Reports 5 (2015).Read more>>
  • Woolf, Eric C., et al. "The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model." PLOS ONE10.6 (2015): e0130357.Read more>>
  • Madka, Venkateshwar, et al. "Targeting mTOR and p53 signaling inhibits muscle invasive bladder cancer in vivo." Cancer Prevention Research 9.1 (2016): 53-62.Read more>>
  • Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.Read more>>
  • Talwar, Harvinder, et al. "MKP-1 negatively regulates LPS-mediated IL-1β production through p38 activation and HIF-1α expression." Cellular Signalling (2017).Read more>>
  • Yang, Ya, et al. "Expression of RAP1B is associated with poor prognosis and promotes an aggressive phenotype in gastric cancer." Oncology reports 34.5 (2015): 2385-2394.Read more>>
  • Talwar, Harvinder, et al. "The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages." Data in Brief (2017).Read more>>
  • Talwar et al. The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages. (2017) Data.Brie. 14:56-61Read more>>
  • Song et al. Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions. (2018) Exp.Ther.Med. 15:5251-5260Read more>>
  • Liu T et al. MicroRNA-493 targets STMN-1 and promotes hypoxia-induced epithelial cell cycle arrest in G/M and renal fibrosis. (2018) FASEB J. Sep 05Read more>>
  • Talwar H et al. MEK2 Negatively Regulates Lipopolysaccharide-Mediated IL-1β Production through HIF-1α Expression. J Immunol. 2019 Mar 15;202(6):1815-1825.Read more>>
  • Yang X et al. Synthesis and bioevaluation of novel 18FDG-conjugated 2-nitroimidazole derivatives for tumor-hypoxia imaging. Mol Pharm. 2019 May 6;16(5):2118-2128. Read more>>
  • Chai D et al. β2-microglobulin has a different regulatory molecular mechanism between ER+ and ER- breast cancer with HER2.BMC Cancer. 2019 Mar 12;19(1):223.Read more>>
  • Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20.Read more>>
  • Wang D et al. Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecularmechanism. Biomed Pharmacother. 2019 Jun 12;117:109061. Read more>>
  • Wu TH et al. The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function. J Oncol. 2019 Nov 3;2019:9206951. Read more>>
  • Li ZH et al. You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. J Ethnopharmacol. 2020 Jan 10;246:112240.Read more>>
  • Shen Y et al. Tumor vasculature remolding by thalidomide increases delivery and efficacy of cisplatin. J Exp Clin Cancer Res. 2019 Oct 28;38(1):427. Read more>>
  • Duda P et al. The Reverse Warburg Effect is Associated with Fbp2-Dependent Hif1α Regulation in Cancer Cells Stimulated by Fibroblasts. Cells. 2020 Jan 14;9(1). pii: E205.Read more>>
  • Ouyang H et al. MicroRNA-208-5p regulates myocardial injury of sepsis mice via targeting SOCS2-mediated NF-κB/HIF-1α pathway. Int Immunopharmacol. 2020 Feb 18;81:106204.Read more>>
  • Sun Y et al. The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism. J Cancer Res Clin Oncol. 2020 May;146(5):1139-1152. Read more>>
  • Rui X et al. Imperative and effective reversion of synovial hyperplasia and cartilage destruction in rheumatoid arthritis through multiple synergistic effects of O2 and Ca2+. Mater Sci Eng C Mater Biol Appl.2020 Sep;114:111058.Read more>>
  • Yongjun Gao. et al. Study on the differential proteomics of rat hippocampal mitochondria during deep hypothermic circulatory arrest. Ann Transl Med. 2021 Feb; 9(4): 346Read more>>
  • Jacopo Di Gregorio. et al. UBXN7 cofactor of CRL3KEAP1 and CRL2VHL ubiquitin ligase complexes mediates reciprocal regulation of NRF2 and HIF-1 proteins. Bba-Mol Cell Res. 2021 Apr;1868:118963Read more>>
  • Jiebin Li. et al. Targeted Temperature Management Suppresses Hypoxia-Inducible Factor-1 and Vascular Endothelial Growth Factor Expression in a Pig Model of Cardiac Arrest. 2021 Jan 05Read more>>
  • Fangmei Zhang. et al. X-ray-triggered NO-released BiCSNO nanoparticles: all-in-one nano-radiosensitizer with photothermal/gas therapy for enhanced radiotherapy. 2020 Aug 31Read more>>
  • Guangrui Chai. et al. NLRP3 Blockade Suppresses Pro-Inflammatory and Pro-Angiogenic Cytokine Secretion in Diabetic Retinopathy. Diabet Metab Synd Ob. 2020; 13: 3047C3058Read more>>
  • Donglin Xia. et al. AuCHemoglobin Loaded Platelet Alleviating Tumor Hypoxia and Enhancing the Radiotherapy Effect with Low-Dose X-ray. Acs Nano. 2020;14(11):15654C15668Read more>>
  • Chen Guanyin. et al. Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits. Stem Cells Int. 2020;2020:8822609Read more>>
  • Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084Read more>>
  • Ying Long. et al. A hybrid membrane coating nanodrug system against gastric cancer via VEGFR2/STAT3 signaling pathway. 2021 Apr 07Read more>>
  • Rui Shao. et al. H3K36 methyltransferase NSD1 regulates chondrocyte differentiation for skeletal development and fracture repair. Bone Res. 2021 Jun;9(1):1-11Read more>>
  • Ayaka Ito. et al. Elevation of Chemosensitivity of Lung Adenocarcinoma A549 Spheroid Cells by Claudin-2 Knockdown through Activation of Glucose Transport and Inhibition of Nrf2 Signal. Int J Mol Sci. 2021 Jan;22(12):6582Read more>>
  • Xiaohua Du. et al. Expression and distribution of neuroglobin and hypoxia-inducible factor-1 in the adult yak telencephalon. 2021 Jun 19Read more>>
  • Zhuowen Yang. et al. Ferrite-encapsulated nanoparticles with stable photothermal performance for multimodal imaging-guided atherosclerotic plaque neovascularization therapy. Biomater Sci-Uk. 2021 JulRead more>>
  • Xuejun Dai. et al. Comparison of the differentiation abilities of bone marrow?derived mesenchymal stem cells and adipose?derived mesenchymal stem cells toward nucleus pulposus?like cells in three?dimensional culture. Exp Ther Med. 2021 Sep;22(3):1-9Read more>>
  • Jianting Yao. et al. Low-Intensity Focused Ultrasound-Responsive Ferrite-Encapsulated Nanoparticles for Atherosclerotic Plaque Neovascularization Theranostics. 2021 Aug 11Read more>>
  • Huaying Hou. et al. NIR-driven intracellular photocatalytic oxygen-supply on metallic molybdenum carbide@N-carbon for hypoxic tumor therapy. J Colloid Interf Sci. 2022 Feb;607:1Read more>>
  • Asl? Okan. et al. Immunoreactive definition of TNF- , HIF-1 , Kir6.2, Kir3.1 and M2 muscarinic receptor for cardiac and pancreatic tissues in a mouse model for type 1 diabetes. Life Sci. 2021 Aug;:119886Read more>>
  • Shiying Huang. et al. Jujube polysaccharides mitigated anemia in rats with chronic kidney disease: Regulation of short chain fatty acids release and erythropoietin production. J Funct Foods. 2021 Nov;86:104673Read more>>
  • Sun Q. et al. PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression.. Front Oncol. 2021 Dec;11:767116-767116Read more>>
  • Sun Tianyu. et al. The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma. J Oncol. 2021;2021:9958483Read more>>
  • Meng-Meng ZHANG. et al. Protective effect of Pai-Nong-San against AOM/DSS-induced CAC in mice through inhibiting the Wnt signaling pathway. Chin J Nat Medicines. 2021 Dec;19:912Read more>>
  • Ze Zhang. et al. Oxygen sensors mediated HIF-1_ accumulation and translocation: A pivotal mechanism of fine particles-exacerbated myocardial hypoxia injury. Environ Pollut. 2022 May;300:118937Read more>>
  • Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1_ and _-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16Read more>>
  • Ma Y. et al. Photobiomodulation Attenuates Neurotoxic Polarization of Macrophages by Inhibiting the Notch1-HIF-1_/NF-_B Signalling Pathway in Mice With Spinal Cord Injury.. Front Immunol. 2022 Mar;13:816952-816952Read more>>
  • Chang Hao. et al. Hypoxic preconditioning improves the survival and pro-angiogenic capacity of transplanted human umbilical cord mesenchymal stem cells via HIF-1_ signaling in a rat model of bronchopulmonary dysplasia. Biochem Bioph Res Co. 2022 MaRead more>>
VALIDATION IMAGES