bs-0730R
[Primary Antibody]
HSP27 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: HSP27
Immunogen Range: 101-205/205
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3315
Swiss Prot: P04792
Source: KLH conjugated synthetic peptide derived from human HSP27
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal Alpha-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 27
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Gao, Hong, et al. "Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprungs disease." International Journal of Experimental Pathology (2014).Read more>>
- Zhang et al. Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma. (2017) Med.Sci.Moni. 23:5246-5253Read more>>
- Zhang X et al. Indolyl-chalcone derivatives induce hepatocellular carcinoma cells apoptosis through oxidative stress related mitochondrial pathway in vitro and in vivo.Chem Biol Interact. 2018 Sep 25;293:61-69. Read more>>
- Qiufang. Zong. et al. Effects of HSP27 gene expression on the resistance to Escherichia coli infection in piglets. Gene. 2021 Mar;773:145415Read more>>
- Xinrui Zhang. et al. Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response. Front Oncol. 2021; 11: 696282Read more>>
- Yuliang Wen. et al. Effect of glycolysis and heat shock proteins on hypoxia adaptation of Tibetan sheep at different altitude. Gene. 2021 Nov;803:145893Read more>>
- Yao Zheng. et al. Comparative proteomic analysis of spleen reveals key immune-related proteins in the yak (Bos grunniens) at different growth stages. Comp Biochem Phys D. 2022 Jun;42:100968Read more>>
- Long Yuan. et al. Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis. ANIM NUTR. 2023 AugRead more>>
- Won Seob Kim. et al. Exploring the impact of temporal heat stress on skeletal muscle hypertrophy in bovine myocytes. J THERM BIOL. 2023 Aug;:103684Read more>>
- Kim Won Seob. et al. Heat Shock Protein 27 Regulates Myogenic and Self-renewal Potential of Bovine Satellite Cells Under Heat Stress. J ANIM SCI. 2023 SepRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated(bs-0730R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
L1 mouse brain lysates L2 mouse liver lysates probed with Anti HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 27kD. Observed band size:27kD.\n
A431 cells probed with HSP27 Polyclonal Antibody, unconjugated (bs-0730R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HSP27 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HSP27 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human Huvec cell lysates ; Lane 5: Human HepG2 cell lysates probed with HSP27 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.