Host: Rabbit
Target Protein: CEAM5
Immunogen Range: 315-365/702
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1048
Swiss Prot: P06731
Source: KLH conjugated synthetic peptide derived from human CEA
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 77
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Zhang, Jing-Jing, et al. "??Proof-of-principle?? concept for ultrasensitive detection of cytokines based on the electrically heated carbon paste electrode."hemical Communications 47.23 (2011): 6551-6553. Read more>>
- Hou, Li, et al. "Graphene oxide-labeled sandwich-type impedimetric immunoassay with sensitive enhancement based on enzymatic 4-chloro-1-naphthol oxidation." Biosensors and Bioelectronics (2013).Read more>>
- Chen, Ze-Zhong, et al. "Indirect immunofluorescence detection of E. coli O157: H7 with fluorescent silica Nanoparticles." Biosensors and Bioelectronics (2014).Read more>>
- Hou, Li, et al. "HCR-stimulated formation of DNAzyme concatamers on gold nanoparticle for ultrasensitive impedimetric immunoassay." Biosensors and Bioelectronics (2015).Read more>>
Antigen: bs-0719P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CEA Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CEAM5 Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEAM5) Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEAM5) Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.