Host: Rabbit
Target Protein: NF-M
Immunogen Range: 101-200/916
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 24588
Swiss Prot: P12839
Source: KLH conjugated synthetic peptide derived from rat NF-M
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 102
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Gao, Yuhua, et al. "Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells." BioMed Research International 2013 (2013).Read more>>
- Yuan, Quan, et al. "Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system." Bioscience, Biotechnology, and Biochemistry (2017): 1-8.Read more>>
- Wang, Xiao-feng. et al. Active constituent of Polygala tenuifolia attenuates cognitive deficits by rescuing hippocampal neurogenesis in APP/PS1 transgenic mice. Bmc Complem Altern M. 2021 Dec;21(1):1-15Read more>>
- Lei Wang. et al. Cognitive impairment is associated with BDNF-TrkB signaling mediating synaptic damage and reduction of amino acid neurotransmitters in heart failure. FASEB J. 2023 Dec;38(1):e23351Read more>>
Formalin-fixed and paraffin embedded rat cochlea tissue labeled Anti-NF-M/Neurofilament M Polyclonal Antibody, Unconjugated (bs-0710R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
293T cells probed with NF-M Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-0710R-A647) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat Cerebellum ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NF-M ) Polyclonal Antibody, Unconjugated (bs-0710R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.