bs-0707R [Primary Antibody]
NF-L Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NF-L

Immunogen Range: 300-350/543


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4747

Swiss Prot: P07196

Source: KLH conjugated synthetic peptide derived from human NH-L intermedial

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 62


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti NF-L/Neurofilament L/Neurofilament 68 Polyclonal Antibody, Unconjugated (bs-0707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Human U251 cell lysates probed with NF-L Polyclonal Antibody, Unconjugated (bs-0707R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.