bs-0672R [Primary Antibody]
c-Kit Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: c-Kit

Immunogen Range: 901-976/976


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3815

Swiss Prot: P10721

Source: KLH conjugated synthetic peptide derived from human CD117/c-kit

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 105


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lu, Taoli, et al. "Electroacupuncture improves behavioral recovery and increases SCF/c-kit expression in a rat model of focal cerebral ischemia/reperfusion." Neurological Sciences (2012): 1-9.Read more>>
  • Zhang, Jun, et al. "Cardiac stem cells differentiate into sinus node-like cells." The Tohoku Journal of Experimental Medicine 222.2 (2010): 113-120.Read more>>
  • Lin, Xue-Lian, et al. "NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF." PloS one 11.2 (2016): e0149031.Read more>>
  • Li, Chuan, et al. "c-kit Positive Cardiac Outgrowth Cells Demonstrate Better Ability for Cardiac Recovery Against Ischemic Myopathy." Journal of stem cell research & therapy7.10 (2017).Read more>>
  • Yin et al. Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation. (2018) Int.J.Mol.Med. 41:649-658Read more>>
  • Ramirez-Garcia-Luna JL et al. Defective bone repair in diclofenac treated C57Bl6 mice with and without lipopolysaccharide induced systemic inflammation.(2018)J. Cell. Physiol. Sep 07Read more>>
  • Gaia Palmini. et al. Analysis of a Preliminary microRNA Expression Signature in a Human Telangiectatic Osteogenic Sarcoma Cancer Cell Line. Int J Mol Sci. 2021 Jan;22(3):1163Read more>>
  • Hui Li. et al. Curcumin Alleviates the Side Effects of Cisplatin on Gastric Emptying of Mice by Inhibiting the Signal Changes of Acetylcholine and Interstitial Cells of Cajal. J Med Food. 2020 Sep;23(9):920-927Read more>>
  • Jiyuan Piao. et al. Substance P blocks -aminopropionitrile-induced aortic injury through modulation of M2 monocyte-skewed monocytopoiesis. Transl Res. 2021 Feb;228:76Read more>>
  • Jianfei Shi. et al. Matrine promotes hepatic oval cells differentiation into hepatocytes and alleviates liver injury by suppression of Notch signalling pathway. Life Sci. 2020 Nov;261:118354Read more>>
  • Peng Lin. et al. Curcumin relieves mice gastric emptying dysfunction induced by L?arginine and atropine through interstitial cells of Cajal. Exp Ther Med. 2021 Jun;21(6):1-6Read more>>
  • Thania R. R. LIMA. et al. Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate. 2022 Mar 07Read more>>
  • aohui Yao. et al. Reproductive Suppression Caused by Spermatogenic Arrest: Transcriptomic Evidence from a Non-Social Animal. INT J MOL SCI. 2023 Jan;24(5):4611Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat kidney tissue labeled Anti-CD117/c-kit/SCFR Polyclonal Antibody, Unconjugated (bs-0672R) at 1:200, followed by conjugation to the secondary antibody and DAB staining