bs-0666R [Primary Antibody]
Fibronectin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Fibronectin

Immunogen Range: 1201-1300/2386


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 14268

Swiss Prot: P11276

Source: KLH conjugated synthetic peptide derived from mouse Fibronectin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 259


Cross Reactive Species: Human
Mouse
Rat
Bovine
Cow

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu, Zhen E., et al. "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." American Journal of Physiology-Renal Physiology 301.4 (2011): F713-F722.Read more>>
  • Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012).Read more>>
  • Jun, Hao, et al. "In vivo and in vitro effects of SREBP-1 on diabetic renal tubular lipid accumulation and RNAi-mediated gene silencing study." Histochemistry and cell biology 131.3 (2009): 327-345.Read more>>
  • Wu, Lina, et al. "The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats." Molecular biology reports 39.9 (2012): 9085-9093.Read more>>
  • Bai, X. P., et al. "Fabrication of engineered heart tissue grafts from alginate/collagen barium composite microbeads." Biomedical Materials 6.4 (2011): 045002.Read more>>
  • Karadeniz, Tugba, et al. "Experimental comparison of protective characteristics of enalapril and trimetazidine in diabetic nephropathy." Renal failure 36.8 (2014): 1283-1290.Read more>>
  • Ma, Yan, et al. "Intraocular expression of serum amyloid a and interleukin-6 in proliferative diabetic retinopathy." American journal of ophthalmology 152.4 (2011): 678-685.Read more>>
  • Elmas, Onur, Oytun Erbas, and Gurkan Yigitturk. "The efficacy of Aesculus hippocastanum seeds on diabetic nephropathy in a streptozotocin-induced diabetic rat model." Biomedicine & Pharmacotherapy 83 (2016): 392-396.Read more>>
  • Kang, Ju Hyung, et al. "Aliskiren Regulates Neonatal Fc Receptor and IgG Metabolism with Attenuation of Anti-GBM Glomerulonephritis in Mice."Nephron (2016).Read more>>
  • Miyauchi, Yuya, et al. "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." Scientific Reports 7 (2017).Read more>>
  • Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950.Read more>>
  • Liu and Xiao Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia. (2017) BMC.Ophthalmo. 17:135Read more>>
  • Wu et al. MicroRNA-27a Induces Mesangial Cell Injury by Targeting of PPARγ, and its In Vivo Knockdown Prevents Progression of Diabetic Nephropathy. (2016) Sci.Rep. 6:26072Read more>>
  • England BR et al. Malondialdehyde‐Acetaldehyde Adducts and Antibody Responses in Rheumatoid Arthritis‐Interstitial Lung Disease. Arthritis Rheumatol. 2019 Apr 1. Read more>>
  • Fasano A et al. HTRA1 expression profile and activity on TGF‐β signaling in HTRA1 mutation carriers. J Cell Physiol. 2020 Feb 4.Read more>>
  • S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062.Read more>>
  • Xiaoyan Yu. et al. Ginkgo biloba leaf extract prevents diabetic nephropathy through the suppression of tissue transglutaminase. Exp Ther Med. 2021 Apr;21(4):1-1Read more>>
  • H-J Wang. et al. MiR-32-5p knockdown inhibits epithelial to mesenchymal transition and renal fibrosis by targeting SMAD7 in diabetic nephropathy:. Hum Exp Toxicol. 0;()Read more>>
  • Siwen Zhang. et al. Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency. Stem Cell Res Ther. 2021 Dec;12(1):1-16Read more>>
  • Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634Read more>>
  • Song Danyang. et al. Silencing of ER-resident oxidoreductase PDIA3 inhibits malignant biological behaviors of multidrug-resistant gastric cancer. Acta Bioch Bioph Sin. 2021 AugRead more>>
  • Leiming Sun. et al. Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats. Bioengineered. 2021;12(2):11188-112Read more>>
  • Yosuke Nagai. et al. Rho-associated, coiled-coilcontaining protein kinase 1 regulates development of diabetic kidney disease via modulation of fatty acid metabolism. KIDNEY INT. 2022 MayRead more>>
  • Ali Doryab. et al. Real-Time Measurement of Cell Mechanics as a Clinically Relevant Readout of an In Vitro Lung Fibrosis Model Established on a Bioinspired Basement Membrane. ADV MATER. 2022 Sep;:2205083Read more>>
  • Xingliang Dai. et al. Crosstalk between microglia and neural stem cells influences the relapse of glioblastoma in GBM immunological microenvironment. CLIN IMMUNOL. 2023 Jun;251:109333Read more>>
  • Demircan Volkan. et al. Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis. SCI REP-UK. 2024 Mar;14(1):1-14Read more>>
VALIDATION IMAGES

Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Fibronectin Polyclonal Antibody (bs-0666R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Paraformaldehyde-fixed, paraffin embedded rat intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated (bs-0666R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining


Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated (bs-0666R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining


Image kindly submitted by Edita Aksamitiene as part of the free sample program. Rat skin lysates probed with Rabbit Anti-Fibronectin Polyclonal Antibody (bs-0666R) at 1:1000.


Formalin-fixed and rat pancreas tissue labeled with Anti-FN/Fibronectin 1 Polyclonal Antibody, Unconjugated (bs-0666R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)


Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Fibronectin Polyclonal Antibody, Unconjugated (bs-0666R) at 1:200 overnight at 4°C, DAB staining.