bs-0662R
[Primary Antibody]
ABCG2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ABCG2
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9429
Source: KLH conjugated synthetic peptide derived from human ABCG2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Su, L., et al. "Effect of colibacillosis or coccidiosis on expression of breast cancer resistance protein in small intestine and liver of chickens." Journal of Veterinary Pharmacology and Therapeutics (2013).Read more>>
- Skoda, Jan, et al. "Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice." Tumor Biology(2016): 1-14.Read more>>
- Duan CY et al. Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells. Cell Mol Biol Lett. 2019 Apr 4;24:3. Read more>>
- Wang J et al. Effects of duration of thermal stress on growth performance, serum oxidative stress indices, the expression and localization of ABCG2 and mitochondria ROS production of skeletal muscle, small intestine and immune organs in broilers. J Therm Biol. 2019 Oct;85:102420.Read more>>
- Le Y et al. Anti-Hyperuricemic Effects of Astaxanthin by Regulating Xanthine Oxidase, Adenosine Deaminase and Urate Transporters in RatsMar Drugs.2020 Dec 1;18(12):610.Read more>>
- Xue Zhou. et al. Let-7b regulates the adriamycin resistance of chronic myelogenous leukemia by targeting AURKB in K562/ADM cells. Leukemia Lymphoma. 2020;61(14):3451-3459Read more>>
- Yan Shou Zhang. et al. Expression of BCRP/ABCG2 Protein in Invasive Breast Cancer and Response to Neoadjuvant Chemotherapy. Oncol Res Treat. Read more>>
- Dongming Chen. et al. Study on the mechanism of Phellinus igniarius total flavonoids in reducing uric acid and protecting uric acid renal injury in vitro. HELIYON. 2023 Jan;9:e12979Read more>>
- Huanhuan Wang. et al. The Pigments in Eggshell with Different Color and the Pigment Regulatory Genes Expression in Corresponding Chickens Shell Gland. ANIMAL. 2023 Mar;:100776Read more>>
- Yuan-Jian Hui. et al. Up-regulation of ABCG2 by MYBL2 deletion drives Chlorin e6-mediated photodynamic therapy resistance in colorectal cancer. PHOTODIAGN PHOTODYN. 2023 Apr;:103558Read more>>
- Xiaofei Zhou. et al. Coffee Leaf Tea Extracts Improve Hyperuricemia Nephropathy and Its Associated Negative Effect in Gut Microbiota and Amino Acid Metabolism in Rats. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti ABCG2/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ABCG2/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated(bs-0662R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
L1 rat brain lysates L2 mouse embryo lysates probed with Anti ABCG2/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 72kD. Observed band size:72kD, 28kD.\n
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Rat Placenta tissue lysates; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Rat Lung tissue lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human HepG2 cell lysates; Lane 9: Human HUVEC cell lysates probed with ABCG2 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.