Host: Rabbit
Target Protein: Connexin 43
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2697
Swiss Prot: P17302
Source: KLH conjugated synthetic peptide derived from human Connexin-43
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
Dog
Predicted Cross Reactive Species:
Cow
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Chen WG, Gao ZW, Ni YH, Dai ZX. Carbenoxolone pretreatment and treatment of posttraumatic epilepsy. Neural Regen Res. 2013;8(2):169-176.Read more>>
- Zhao, Jing, et al. "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." Basic Research in Cardiology 109.5 (2014): 1-13.Read more>>
- Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
- Chen et al. Carbenoxolone pretreatment and treatment of posttraumatic epilepsy. (2013) Neural.Regen.Res. 8:169-76Read more>>
- Zhan C et al. Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Toxicol Lett. 2019 Oct 1. pii: S0378-4274(19)30295-4. Read more>>
- Inayatilah F et al. Monoclonal antibodies of human zona pellucida 3 (MabhZP3) as immunocontraception candidate on connexin expression 43 (Cx43) in granulosa ovarian cell and luteinizing hormone level in the blood serum of mice (Mus musculus). Drug Invention Today.12 (11)2019.Read more>>
- Hui Li. et al. Curcumin Alleviates the Side Effects of Cisplatin on Gastric Emptying of Mice by Inhibiting the Signal Changes of Acetylcholine and Interstitial Cells of Cajal. J Med Food. 2020 Sep;23(9):920-927Read more>>
- Peng Lin. et al. Curcumin relieves mice gastric emptying dysfunction induced by L?arginine and atropine through interstitial cells of Cajal. Exp Ther Med. 2021 Jun;21(6):1-6Read more>>
- Zhouxia Luo. et al. CAPE-pNO2 ameliorates diabetic brain injury through modulating Alzheimer's disease key proteins, oxidation, inflammation and autophagy via a Nrf2-dependent pathway. Life Sci. 2021 Dec;287:119929Read more>>
- ohui Xiong. et al. LncRNA8276 primes cellCcell adhesion for regulation of spermatogenesis. ANDROLOGY-US. 2022 SepRead more>>
- Hanhan Fang. et al. Gut-Spleen Axis: Microbiota via Vascular and Immune Pathways Improve Busulfan-Induced Spleen Disruption | mSphere. MSPHERE. 2022 DecRead more>>
- Xu Yang. et al. Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc?/GPX4 axis. FOOD CHEM TOXICOL. 2023 May;175:11373Read more>>
- Zheng Meng. et al. Targeted ablation of the left middle cervical ganglion prevents ventricular arrhythmias and cardiac injury induced by AMI. BASIC RES CARDIOL. 2023 Dec;:1-18Read more>>
Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-Connexin 43 Polyclonal Antibody, Unconjugated (bs-0651R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-Connexin 43 Polyclonal Antibody, Unconjugated (bs-0651R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.
MCF-7 cells were stained with Connexin 43 Polyclonal Antibody, Unconjugated(bs-0651R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Hela cells probed with Connexin 43 Polyclonal Antibody, unconjugated (bs-0651R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Tissue/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Connexin 43) polyclonal Antibody, Unconjugated (bs-0651R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Mouse Bladder lysates probed with Connexin 43 Polyclonal Antibody, Unconjugated (bs-0651R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.