bs-0649R [Primary Antibody]
CD68 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD68

Immunogen Range: 21-120/335


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12514

Swiss Prot: P31996

Source: KLH conjugated synthetic peptide derived from mouse CD68

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zeng, Wei-qun, et al. "A New Method to Isolate and Culture Rat Kupffer Cells." PLOS ONE 8.8 (2013): e70832.Read more>>
  • Chen, Liang, et al. "Electrospun Poly (L-lactide)/Poly (ε-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells." PLOS ONE 8.8 (2013): e71265.Read more>>
  • Hong, Hye Kyoung, et al. "Neonatal systemic inflammation in rats alters retinal vessel development and simulates pathologic features of retinopathy of prematurity." Journal of Neuroinflammation 11.1 (2014): 87.Read more>>
  • Wang, Shan, et al. "5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts." Ultrasound in Medicine & Biology (2014).Read more>>
  • Savvatis, Konstantinos, et al. "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." Basic Research in Cardiology 109.6 (2014): 1-14.Read more>>
  • Xiao, Ke, et al. "The role of visfatin on the regulation of inflammation and apoptosis in the spleen of LPS-treated rats." Cell and Tissue Research (2014).Read more>>
  • Bang, Mi-Ae, et al. "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." PLoS one 10.2 (2014): e0117524-e0117524.Read more>>
  • Shang, Qianwen, et al. "Delivery of Adipose-derived Stem Cells Attenuates Adipose Tissue Inflammation and Insulin Resistance in Obese Mice through Remodeling Macrophage Phenotypes." Stem Cells and Development (2015).Read more>>
  • Xu, Xiaoyang, et al. "Lysosomal cholesterol accumulation in macrophages leading to coronary atherosclerosis in CD38−/− mice." Journal of Cellular and Molecular Medicine (2016).Read more>>
  • Kang, Yea Eun, et al. "The Roles of Adipokines, Proinflammatory Cytokines, and Adipose Tissue Macrophages in Obesity-Associated Insulin Resistance in Modest Obesity and Early Metabolic Dysfunction." PLOS ONE 11.4 (2016): e0154003.Read more>>
  • Xu, Xiaoyang, et al. "Simvastatin promotes NPC1‐mediated free cholesterol efflux from lysosomes through CYP7A1/LXRα signalling pathway in oxLDL‐loaded macrophages." Journal of Cellular and Molecular Medicine (2016).Read more>>
  • Wang, Yu‑Hai, et al. "Reduced inflammatory cell recruitment and tissue damage in spinal cord injury by acellular spinal cord scaffold seeded with mesenchymal stem cells." Experimental and Therapeutic Medicine.Read more>>
  • Stark, Christoffer, et al. "Thymosin beta 4 treatment improves left ventricular function after myocardial infarction and is related to Up-regulation of chitinase 3-like-1 in mice." Translational Medicine Communications 1.1 (2016): 8.Read more>>
  • Ganguly, Rituparna, et al. "Oral chromium picolinate impedes hyperglycemia-induced atherosclerosis and inhibits proatherogenic protein TSP-1 expression in STZ-induced type 1 diabetic ApoE−/− mice." Scientific Reports 7 (2017).Read more>>
  • Kugo, Hirona, et al. "Suppressive effects of dietary EPA-rich fish oil on the degradation of elastin fibers in the aortic wall in nicotine-administered mice." Food & Function (2017).Read more>>
  • Vendrov, Aleksandr E., et al. "Attenuated Superoxide Dismutase 2 Activity Induces Atherosclerotic Plaque Instability During Aging in Hyperlipidemic Mice." Journal of the American Heart Association 6.11 (2017): e006775.Read more>>
  • Müller, Irene, et al. "Pathogenic Role of the Damage-Associated Molecular Patterns S100A8 and S100A9 in Coxsackievirus B3–Induced Myocarditis." Circulation: Heart Failure 10.11 (2017): e004125.Read more>>
  • Wang et al. Programmed cell death-4 deficiency prevents diet-induced obesity, adipose tissue inflammation, and insulin resistance. (2013) Diabete. 62:4132-43Read more>>
  • Wu et al. Chronic inflammation exacerbates glucose metabolism disorders in C57BL/6J mice fed with high-fat diet. (2013) J.Endocrino. 219:195-204Read more>>
  • Chen et al. Association of Tartrate-Resistant Acid Phosphatase-Expressed Macrophages and Metastatic Breast Cancer Progression. (2015) Medicine.(Baltimore. 94:e2165Read more>>
  • Nishizawa et al. IGF-I induces senescence of hepatic stellate cells and limits fibrosis in a p53-dependent manner. (2016) Sci.Re. 6:34605Read more>>
  • Nishikawa,et al.Highly Dispersible and Bioavailable Curcumin but not Native Curcumin Induces Brown-Like Adipocyte Formation in Mice.(2018) Molecular Nutrition & Food Research. 62:.Read more>>
  • Zhou,et al.Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect.(2018) Biosensors & Bioelectronics. 106:99-104.Read more>>
  • "Guo G et al. Radical polymerization-crosslinking method for improving extracellular matrix stability in bioprosthetic heart valves with reduced potential for calcification and inflammatory response.(2018) Acta Biomater. Oct 14. pii: S1742-7061(18)30616-0. "Read more>>
  • Zhao X et al. Protective effects of dioscin against systemic inflammatory response syndromevia adjusting TLR2/MyD88/NF‑κb signal pathway.(2018)Int. Immunopharmacol.Dec;65:458-469. Read more>>
  • Luo Y et al. MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Brain Res Bull. 2019 Mar;146:320-326. Read more>>
  • Guo G et al. Hydrogel hybrid porcine pericardium for the fabrication of pre-mounted TAVI valve with improved biocompatibility. Journal of Materials Chemistry B. 2019.Read more>>
  • Zhang J et al. Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation. Toxicology. 2019 Mar 1;415:37-48.Read more>>
  • Nishikawa S et al. Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively Activated Macrophages in Mice. J Nutr Sci Vitaminol (Tokyo). 2019;65(4):328-334. Read more>>
  • Sukketsiri W et al. Isoflavone Ameliorated Oxidative Stress and Vascular Damages in Nicotine-Administrated Mice. J Oleo Sci. 2019 Dec 1;68(12):1241-1249.Read more>>
  • Bok SH et al. Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2‑related factors in an ovalbumin‑induced asthma mouse model. Mol Med Rep. 2019 Aug 6. Read more>>
  • Wu P et al. Fluoride induces autoimmune orchitis involved with enhanced IL-17A secretion in mice testis. J Agric Food Chem. 2019 Dec 4;67(48):13333-13343.Read more>>
  • Nakayama Y et al. Receptor activator of the NFκB ligand system protects renal function during experimental renal ischemia-reperfusion in mice. Transpl Immunol. 2020 Feb;58:101263.Read more>>
  • Linhe Jinet al. Tough pNAGA hydrogel hybridized porcine pericardium for the pre-mounted TAVI valve with improved anti-tearing properties and hemocompatibility. Biomed Mater. 2020 Oct 3;15(6):065013.Read more>>
  • Yu Satoet al. Prophylactic therapy with Human amniotic fluid stem cells improved survival in a rat model of lipopolysaccharide-induced neonatal sepsis through immunomodulation via aggregates with peritoneal macrophages. Stem Cell Res Ther. 2020 Jul 20;11(1):300.Read more>>
  • Yang Fan. et al. Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation. Evid-Based Compl Alt. 2021;2021:6657673Read more>>
  • Lidija Gradi?nik. et al. Isolation, characterisation and phagocytic function of human macrophages from human peripheral blood. Mol Biol Rep. 2020 Sep;47(9):6929-694Read more>>
  • Patrick Ming-Kuen Tang. et al. Neural transcription factor Pou4f1 promotes renal fibrosis via macrophageCmyofibroblast transition. P Natl Acad Sci Usa. 2020 Aug;117(34):20741-20752Read more>>
  • Meng Xiangkun. et al. Deficiency of cysteinyl cathepsin K suppresses the development of experimental intimal hyperplasia in response to chronic stress. J Hypertens. 2020 Aug;38(8):1514-1524Read more>>
  • inggang Wu. et al. Extracellular matrix coating improves the biocompatibility of polymeric heart valves. J Mater Chem B. 2020 Dec;8(46):10616-10629Read more>>
  • Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084Read more>>
  • Hong Zhen. et al. VitA or VitD ameliorates bronchopulmonary dysplasia by regulating the balance between M1 and M2 macrophages. Biomed Pharmacother. 2021 Sep;141:111836Read more>>
  • Tang Gao. et al. Biomimetic Glucan Particles with Aggregation-Induced Emission Characteristics for Noninvasive Monitoring of Transplant Immune Response. Acs Nano. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Zhou, Hang. et al. AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury. J Neuroinflamm. 2021 Dec;18(1):1-17Read more>>
  • Yibei Wang. et al. NOD2 deficiency confers a pro-tumorigenic macrophage phenotype to promote lung adenocarcinoma progression. 2021 Jul 16Read more>>
  • Fan Yang. et al. Visible lightCinduced cross-linking of porcine pericardium for the improvement of endothelialization, anti-tearing, and anticalcification properties. 2021 Jul 09Read more>>
  • Gentaro Yamasaki. et al. Effect of FURIN SNP rs17514846 on coronary atherosclerosis in human cardiac specimens: An autopsy study of 106 cases. Legal Med-Tokyo. 2022 Mar;55:102006Read more>>
  • Xue Gong. et al. Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. J Clin Invest. 2021 Dec 15;131(24):e147031Read more>>
  • Ivana Dokic. et al. Neuroprotective Effects of Ultra-High Dose Rate FLASH Bragg Peak Proton Irradiation. Int J Radiat Oncol. 2022 FebRead more>>
  • Kailei Ding. et al. A PEGylation method of fabricating bioprosthetic heart valves based on glutaraldehyde and 2-amino-4-pentenoic acid co-crosslinking with improved antithrombogenicity and cytocompatibility. Acta Biomater. 2022 MaRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat myocardium labeled with Anti-MuRF1/Trim63 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and rat colitis tissue labeled with Anti-CD68 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5),


Paraformaldehyde-fixed, paraffin-embedded Mouse Kidney issue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with CD68 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.


Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.