VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated(bs-0648R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody (bs-0648R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:250.
Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-CD8 Antibody, conjugated (bs-0648R-AF555) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: CD8 Antibody, conjugated (bs-0648R-AF488) 1:400, 90 minutes at 37°C; DAPI (5ug/ml, blue) was used to stain the cell nuclei.
Lane 1: Mouse Spleen; 40ug loaded in each lane; Probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.
Lane 1: Jurkat cell lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: mCD8a overexpression 293T cell lysates probed with CD8a Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Lymph node tissue lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Molt-4 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD8 Antibody(bs-0648R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).