bs-0648R [Primary Antibody]
CD8 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD8

Immunogen Range: 51-150/247


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12525

Swiss Prot: P01731

Source: KLH conjugated synthetic peptide derived from mouse CD8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 27


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • He, Qiushan, et al. "Low-dose paclitaxel enhances the anti-tumor efficacy of GM-CSF surface-modified whole-tumor-cell vaccine in mouse model of prostate cancer." Cancer Immunology, Immunotherapy 60.5 (2011): 715-730.Read more>>
  • Zhang, Wen, et al. "The role of CXCR3 in the induction of primary biliary cirrhosis." Clinical and Developmental Immunology 2011 (2011).Read more>>
  • Lee M-F, Yang K-J, Wang NM, Chiu Y-T, Chen P-C, et al. (2014) The Development of a Murine Model for Forcipomyia taiwana (Biting Midge) Allergy. PLoS ONE 9(3): e91871Read more>>
  • Jeong, Keun-Yeong, et al. "Combination of macrophage inflammatory protein 1 alpha with existing therapies to enhance the antitumor effects on murine hepatoma." Journal of Radiation Research (2014): rru077.Read more>>
  • Ashraf, M. I., et al. "Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation." American Journal of Transplantation (2015).Read more>>
  • Kovtun, A., et al. "The crucial role of neutrophil granulocytes in bone fracture healing." European Cells and Materials 32 (2016):152-162.Read more>>
  • Li, Yan, et al. "Immunogenic FEAT protein circulates in the bloodstream of cancer patients." Journal of Translational Medicine 14.1 (2016): 275.Read more>>
  • Ju, Huanyu, et al. "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." BMC immunology 17.1 (2016): 31.Read more>>
  • Wang, Raymond M., et al. "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." Biomaterials (2017).Read more>>
  • Qiao, Xinwei, et al. "Increased expression of Tim-3 and its ligand Galectin-9 in rat allografts during acute rejection episodes." Biochemical and biophysical research communications 445.3 (2014): 542-548.Read more>>
  • Chuang, Meng-Hsien, et al. "The Chinese medicine JC-001 enhances the chemosensitivity of Lewis lung tumors to cisplatin by modulating the immune response." BMC Complementary and Alternative Medicine 17.1 (2017): 210.Read more>>
  • Haffner-Luntzer, Melanie, et al. "The inflammatory phase of fracture healing is influenced by oestrogen status in mice." European Journal of Medical Research 22.1 (2017): 23.Read more>>
  • Jaime-Ramirez, Alena C., et al. "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." Molecular Therapy-Oncolytics 5 (2017): 87-96.Read more>>
  • Kroner, Jochen, et al. "Mast cells are critical regulators of bone fracture‐induced inflammation and osteoclast formation and activity." Journal of Bone and Mineral Research (2017).Read more>>
  • Tanaka, Rui, et al. "The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model." American Journal of Cancer Research 7.8 (2017): 1693.Read more>>
  • Kovtun, Anna, et al. "Complement receptors C5aR1 and C5aR2 act differentially during the early immune response after bone fracture but are similarly involved in bone repair." Scientific Reports 7.1 (2017): 14061.Read more>>
  • Prystaz, Katja, et al. "Distinct effects of interleukin-6 classic and trans-signaling in bone fracture healing." The American Journal of Pathology.Read more>>
  • Dong et al. Antitumor effect of embryonic stem cells in a non-small cell lung cancer model: antitumor factors and immune responses. (2013) Int.J.Med.Sc. 10:1314-20Read more>>
  • Wang et al. Targeted Disruption of the β2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells. (2015) Stem.Cells.Transl.Me. 4:1234-45Read more>>
  • Jaime-Ramirez et al. Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. (2017) Mol.Ther.Oncolytics. 5:87-96Read more>>
  • Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31Read more>>
  • Schmid et al. Acute Renal Graft-Versus-Host Disease in a Murine Model of Allogeneic Bone Marrow Transplantation. (2017) Cell.Transplant. 26:1428-1440Read more>>
  • Simanovich et al. Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation. (2017) Oncoimmunology. 6:e1261778Read more>>
  • Ashraf et al. Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation. (2016) Am.J.Transplant. 16:808-20Read more>>
  • Mödinger, Y., et al. “Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture Healing.” The American Journal of Pathology (2018).Read more>>
  • Zhu,et al.Co-delivery of tumor antigen and dual toll-like receptor ligands into dendritic cell by silicon microparticle enables efficient immunotherapy against melanoma.(2018) Journal of Controlled Release. 272:72-82.Read more>>
  • Wang,et al.Black tea and D. candidum extracts play estrogenic activity via estrogen receptor α-dependent signaling pathway.(2018) American Journal of Translational Research. 10:114-125.Read more>>
  • "Mödinger Y et al. Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture Healing. (2018) Am J Pathol. Oct 17. "Read more>>
  • Rapp et al. Induced global deletion of glucocorticoid receptor impairs fracture healing. (2018) FASEB.J. 32:2235-2245Read more>>
  • Zhang H et al. Utilizing VEGF165b mutant as an effective immunization adjunct to augment antitumor immune response.Vaccine. 2019 Apr 3;37(15):2090-2098.Read more>>
  • Wan WJ et al. Doxorubicin and siRNA-PD-L1 co-delivery with T7 modified ROS-sensitive nanoparticles for tumor chemoimmunotherapy. Int J Pharm. 2019 Jul 20;566:731-744. Read more>>
  • Demontoux L et al. Hypotonic stress enhances colon cancer cell death induced by platinum derivatives and immunologically improves anti‐tumor efficacy of intraperitoneal chemotherapy. Int J Cancer. 2019 Jul 25.Read more>>
  • Sun J1 et al. Clinical effects of lentinan combined with budesonide inhalation in treating acute exacerbation of chronic obstructive pulmonary disease under mechanical ventilation. Exp Ther Med. 2019 Mar;17(3):1503-1508. Read more>>
  • Zhang Y et al. Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway. J Cell Mol Med. 2019 Nov 15.Read more>>
  • Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110. Read more>>
  • Li TF et al. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma.Biomaterials. 2018 Oct;181:35-52.Read more>>
  • Yihan Lu? et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563.Read more>>
  • Zhao B et al. A TCM Formula YYWY Inhibits Tumor Growth in Non-Small Cell Lung Cancer and Enhances Immune-Response Through Facilitating the Maturation of Dendritic Cells. Front Pharmacol. 2020 Jun 9;11:798.Read more>>
  • oon Ching Teeet al. Xenogeneic mesenchymal stem cell transplantation for mandibular defect regeneration. Xenotransplantation. 2020 Sep;27(5):e12625.Read more>>
  • Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28Read more>>
  • Noritaka Yonezawa. et al. Abscopal Effect of Frozen Autograft Reconstruction Combined with an Immune Checkpoint Inhibitor Analyzed Using a Metastatic Bone Tumor Model. Int J Mol Sci. 2021 Jan;22(4):1973Read more>>
  • Wenli Fang. et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 + T cell exclusion. J Exp Clin Canc Res. 2021 Dec;40(1):1-11Read more>>
  • Christophe Glorieux. et al. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling. Redox Biol. 2021 Jan;38:10178Read more>>
  • Di Zhao. et al. Chromatin Regulator CHD1 Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer. Cancer Discov. 2020 Sep;10(9):1374-1387Read more>>
  • Muhammad Ishfaq. et al. Baicalin attenuated Mycoplasma gallisepticum\induced immune impairment in chicken bursa of fabricius through modulation of autophagy and inhibited inflammation and apoptosis. J Sci Food Agr. 2021 Feb;101(3):880-89Read more>>
  • Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306Read more>>
  • Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211Read more>>
  • Miss Hongfang Meng. et al. Overexpression of hepatocyte growth factor in dental pulp stem cells ameliorates the severity of psoriasis by reducing inflammatory responses. 2021 Jun 22Read more>>
  • Weili Xu. et al. Targeted elimination of myeloid-derived suppressor cells via regulation of the STAT pathway alleviates tumor immunosuppression in neuroblastoma. Immunol Lett. 2021 Dec;240:31Read more>>
  • Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated(bs-0648R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody (bs-0648R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:250.


Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-CD8 Antibody, conjugated (bs-0648R-AF555) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: CD8 Antibody, conjugated (bs-0648R-AF488) 1:400, 90 minutes at 37°C; DAPI (5ug/ml, blue) was used to stain the cell nuclei.


Lane 1: Mouse Spleen; 40ug loaded in each lane; Probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.


Lane 1: Jurkat cell lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: mCD8a overexpression 293T cell lysates probed with CD8a Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Lymph node tissue lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.


Molt-4 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD8 Antibody(bs-0648R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).