bs-0647R-PE [Conjugated Primary Antibody]
CD4 Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD4

Immunogen Range: 385-457/457


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24932

Swiss Prot: P01730

Source: KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat
Pig
Chicken

Predicted Cross Reactive Species: Dog
Cow
Sheep
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Smit, Francis Edwin, et al. "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?." Cell and Tissue Banking: 1-14.Read more>>
  • Jaime-Ramirez, Alena C., et al. "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." Molecular Therapy-Oncolytics 5 (2017): 87-96.Read more>>
  • Jaime-Ramirez et al. Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. (2017) Mol.Ther.Oncolytics. 5:87-96Read more>>
  • Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.Read more>>
  • Hu X et al. Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus. Chemosphere. 2019 Jul 22;237:124427. Read more>>
  • Sun J1 et al. Clinical effects of lentinan combined with budesonide inhalation in treating acute exacerbation of chronic obstructive pulmonary disease under mechanical ventilation. Exp Ther Med. 2019 Mar;17(3):1503-1508. Read more>>
  • Wenli Fang. et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 + T cell exclusion. J Exp Clin Canc Res. 2021 Dec;40(1):1-11Read more>>
  • Maria Angelica Cortez. et al. Bone morphogenetic protein 7 promotes resistance to immunotherapy. Nat Commun. 2020 Sep;11(1):1-14Read more>>
  • Rosiris Len-Rivera. et al. Central Nervous System (CNS) Viral Seeding by Mature Monocytes and Potential Therapies To Reduce CNS Viral Reservoirs in the cART Era. Mbio. 2021 Apr;12(2):e03633-2Read more>>
  • Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211Read more>>
  • Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26Read more>>
  • Nie, Ding. et al. The intestinal flora of patients with GHPA affects the growth and the expression of PD-L1 of tumor. 2021 Oct 13Read more>>
  • Kousuke Umeda. et al. Transcriptomic Analysis of the Effects of Chemokine Receptor CXCR3 Deficiency on Immune Responses in the Mouse Brain during Toxoplasma gondii Infection. Microorganisms. 2021 Nov;9(11):234Read more>>
  • Weidan Fang. et al. microRNA-128-3p inhibits CD4+ regulatory T cells enrichment by targeting interleukin 16 in gastric cancer. Bioengineered. 2022;13(1):1025-1038Read more>>
  • Long-Yi Chan. et al. Cre/LoxP Genetic Recombination Sustains Cartilage Anabolic Factor Expression in Hyaluronan Encapsulated MSCs Alleviates Intervertebral Disc Degeneration. Biomedicines. 2022 Mar;10(3):555Read more>>
  • Weiqi Meng. et al. ZhenQi FuZheng formula inhibits the growth of colorectal tumors by modulating intestinal microflora-mediated immune function. AGING-US. 2022 Jun 15; 14(11): 47694785Read more>>
  • Xu Jun Yan. et al. Interleukin-5-induced eosinophil population improves cardiac function after myocardial infarction. CARDIOVASC RES. 2022 Jul;118(9):2165-2178Read more>>
  • Ryohei Annen. et al. Tumor-Specific Immunoenhancing Effects after Local Cryoablation for Metastatic Bone Tumor in a Mouse Model. INT J MOL SCI. 2022 Jan;23(16):9445Read more>>
  • Sun, Xinxin. et al. 0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats. ODONTOLOGY. 2022 Sep;:1-11Read more>>
  • Shuang Zhou. et al. Tumor microenvironment adrenergic nerves blockade liposomes for cancer therapy. J CONTROL RELEASE. 2022 Nov;351:656Read more>>
  • Prema Robinson. et al. Genetic and Small-Molecule Modulation of Stat3 in a Mouse Model of Crohn’s Disease. J CLIN MED. 2022 Jan;11(23):702Read more>>
  • Gao Jie-Fang. et al. Myricetin treatment has ameliorative effects in DNFB-induced atopic dermatitis mice under high-fat conditions. TOXICOL SCI. 2022 DecRead more>>
  • Li, Jun. et al. Immunoproteasome inhibition prevents progression of castration-resistant prostate cancer. BRIT J CANCER. 2023 Jan;:1-14Read more>>
  • Jia-qi Yuan. et al. S100A9 promotes glycolytic activity in HER2-positive breast cancer to induce immunosuppression in the tumour microenvironment. HELIYON. 2023 Feb;9:e13294Read more>>
  • Qing Yao. et al. Water-responsive gel extends drug retention and facilitates skin penetration for curcumin topical delivery against psoriasis. ASIAN J PHARM SCI. 2023 Feb;:100782Read more>>
  • Wenkai Ren. et al. Echinacea purpurea-derived homogeneous polysaccharide exerts anti-tumor efficacy via facilitating M1 macrophage polarization. The Innovation. 2023 Mar 13; 4(2): 100391Read more>>
  • Prema Robinson. et al. Therapeutic Potential of a Small-Molecule STAT3 Inhibitor in a Mouse Model of Colitis. CANCERS. 2023 Jan;15(11):2977Read more>>
  • Quanyu Chen. et al. Comparative effects of hepatocyte growth factor and tacrolimus on acute liver allograft early tolerance. FRONT IMMUNOL. 2023; 14: 1162439Read more>>
  • Nahyun Choi. et al. Involvement of DKK1 secreted from adipose-derived stem cells in alopecia areata. CELL PROLIFERAT. 2023 Nov;:e13562Read more>>
  • Kasem Shaimaa M.. et al. Prophylactic and Therapeutic Efficacy of Ultrasonicated Rosmarinus officinalis Ethanolic Extract and its Chitosan-Loaded Nanoparticles Against Eimeria tenella Infected Broiler Chickens. ACTA PARASITOL. 2024 Mar;:1-49Read more>>
VALIDATION IMAGES