DATASHEET
Host:
Rabbit
Target Protein:
GFP
Immunogen Range:
151-238/238
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
107331116
Swiss Prot:
P42212
Source:
KLH conjugated synthetic peptide derived from belt jellyfish GFP
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
PRODUCT SPECIFIC PUBLICATIONS
- Li, Z-G., et al. "Knockdown of Porcine Endogenous Retroviruses by RNA Interference in Chinese Experimental Miniature Pig Fibroblasts." Transplantation Proceedings. Vol. 45. No. 2. Elsevier, 2013.Read more>>
- Radics, Julia, Lisa Königsmaier, and Thomas C. Marlovits. "Structure of a pathogenic type 3 secretion system in action." Nature structural & molecular biology (2014).Read more>>
- Mou C et al. Identification of Nuclear Localization Signals in the ORF2 Protein of Porcine Circovirus Type 3. Viruses. 2019 Nov 22;11(12). pii: E1086. Read more>>
VALIDATION IMAGES
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P); Lane 2: Control (-) E.coli lysates probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: GFP-tagged fusion protein probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:20000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GFP-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-33009P) probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:1000~10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.