bs-0638R [Primary Antibody]
TGF Beta R1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TGF beta Receptor 1

Immunogen Range: 301-400/501


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7046

Swiss Prot: P36897

Source: KLH conjugated synthetic peptide derived from human TGF-beta R1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse
Rat
Bovine

Predicted Cross Reactive Species: Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xie, Weihan, et al. "Regulation of cellular behaviors of fibroblasts related to wound healing by sol‐gel derived bioactive glass particles." Journal of Biomedical Materials Research Part A (2016).Read more>>
  • Yang, Xiao, et al. "Effect of Iodine Excess on Th1, Th2, Th17, and Treg Cell Subpopulations in the Thyroid of NOD. H-2h4 Mice." Biological Trace Element Research (2014): 1-9.Read more>>
  • Tang, K., et al. "GDF9 affects the development and tight junction functions of immature bovine Sertoli cells." Reproduction in Domestic Animals (2017).Read more>>
  • Cashman et al. SENP5 mediates breast cancer invasion via a TGFβRI SUMOylation cascade. (2014) Oncotarge. 5:1071-82Read more>>
  • Li et al. TNF-α enhances the effect of TGF-β on Gli2 expression in the KG-1 leukemic cell line. (2014) Exp.Ther.Me. 8:676-680Read more>>
  • Yan Zheng. et al. Downregulation of Rap1GAP Expression Activates the TGF-/Smad3 Pathway to Inhibit the Expression of Sodium/Iodine Transporter in Papillary Thyroid Carcinoma Cells. Biomed Res Int. 2021;2021:6840642Read more>>
  • Hee Ho Song. et al. Effects of fish collagen on hairless mice skin photoaging induced by ultraviolet irradiation via regulation of the TGF- signaling pathway: Anti-photoaging effect of fish collagen in UVB-induced hairless mice. J FUNCT FOODS. 2023 Jun;105:105554Read more>>
  • Qinggen XIONG. et al. Hypoxia-induced endothelial cell-derived exosome stimulates vascular smooth muscle cell proliferation and migration. BIOMED RES-TOKYO. 2023 Nov;44(6):245-255Read more>>
  • Minhai Dong. et al. Hypoxia-induced TREM1 promotes mesenchymal-like states of glioma stem cells via alternatively activating tumor-associated macrophages. CANCER LETT. 2024 Mar;:216801Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-TGF Beta R1/TGFBR1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


L1 rat brain lysates L2 mouse embryo lysates probed with Anti TGF Beta R1/TGFBR1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 55kD. Observed band size:55kD.\n


Lane 1: Mouse Placenta lysates; Lane 2: Rat Small intestine lysates probed with TGF Beta R1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.