bs-0629R [Primary Antibody]
Estrogen Sulfotranferase Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Estrogen Sulfotransferase

Immunogen Range: 51-145/294


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6783

Swiss Prot: P49888

Source: KLH conjugated synthetic peptide derived from human Estrogen Sulfotranferase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 2 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti SULT1E1/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti SULT1E1/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


HepG2 probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


MCF-7 Cell lysates probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.