bs-0623R-Cy5 [Conjugated Primary Antibody]
Cyclin D1 Polyclonal Antibody, Cy5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cyclin D1

Immunogen Range: 61-110/295


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12443

Swiss Prot: P25322

Source: KLH conjugated synthetic peptide derived from mouse Cyclin D1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Mouse
Rat
Bovine
Sheep
Rabbit

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
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  • Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464.Read more>>
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  • Shi, Yongguo, et al. "Long non-coding RNA Loc554202 regulates proliferation and migration in breast cancer cells." Biochemical and Biophysical Research Communications (2014).Read more>>
  • Lin, Caiyu, et al. "Lithocarpus Polystachyus Rehd Leaf Aqueous Extract Inhibits Human Breast Cancer Growth In Vitro and In Vivo." Nutrition and Cancer (2014).Read more>>
  • Wang, Haihe, et al. "RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway." Biochemical and Biophysical Research Communications (2014).Read more>>
  • He, Xiangming, et al. "CDK2-AP1 inhibits growth of breast cancer cells by regulating cell cycle and increasing docetaxel sensitivity in vivo and in vitro." Cancer Cell International 14.1 (2014): 130.Read more>>
  • Souza, Liliana R., et al. "Genistein Protects Hematopoietic Stem Cells against G-CSF–Induced DNA Damage." Cancer Prevention Research 7.5 (2014): 534-544.Read more>>
  • Zhang, Jihong, et al. "Interleukin 18 augments growth ability via NF-κB and p38/ATF2 pathways by targeting cylin B1, cyclin B2, cylin A2, and Bcl-2 in BRL-3A rat liver cells." Gene (2015).Read more>>
  • Yang, Ning, et al. "SOX 1, contrary to SOX 2, suppresses proliferation, migration, and invasion in human laryngeal squamous cell carcinoma by inhibiting the Wnt/β-catenin pathway." Tumor Biology (2015): 1-11.Read more>>
  • Wang, Yang, et al. "Total flavonoid aglycones extract in Radix Scutellariae inhibites lung carcinoma and lung metastasis by affecting cell cycle and DNA synthesis." Journal of Ethnopharmacology (2016).Read more>>
  • Jiang, Xiao-Hua, et al. "Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/β-catenin pathway." BMC microbiology 17.1 (2017): 124.Read more>>
  • Huang, Chao, et al. "Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis. Corrigendum in/10.3892/mmr. 2016.5882." Molecular Medicine Reports 14.3 (2016): 2555-2565.Read more>>
  • Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).Read more>>
  • Zhang et al. The novel protein C3orf43 accelerates hepatocyte proliferation. (2017) Cell.Mol.Biol.Let. 22:21Read more>>
  • Liang et al. Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells. (2014) Oncotarge. 5:5798-807Read more>>
  • Carreras et al. Chronic sleep fragmentation induces endothelial dysfunction and structural vascular changes in mice. (2014) Slee. 37:1817-24Read more>>
  • Wu et al. The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma. (2015) Oncotarge. 6:9160-72Read more>>
  • He et al. Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2. (2018) Oncol.Lett. 15:3977-3984Read more>>
  • Chang et al. Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level. (2015) BMC.Cell.Biol. 16:25Read more>>
  • Chen et al. STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7. (2015) Cancer.Cell.Int. 15:111Read more>>
  • He et al. Bradykinin potentially stimulates cell proliferation in rabbit corneal endothelial cells through the ZO‑1/ZONAB pathway. (2018) Int.J.Mol.Med. Read more>>
  • Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3.Read more>>
  • Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3.Read more>>
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  • Xiong Y et al. ZYZ-803, a novel hydrogen sulfide-nitric oxide conjugated donor, promotes angiogenesis via cross-talk between STAT3 and CaMKII. Acta Pharmacol Sin. 2019 Jul 17. Read more>>
  • Zhao TY et al. Recovery of Chemotherapy-induced Anemia by Angelica Polysaccharides in C57BL/6N Mice and Elucidation of Underlying Mechanism. Pakistan J. Zool. 2019 51, 5:1927-1935.Read more>>
  • Li Q et al. All-trans retinoic acid regulates sheep primary myoblasts proliferation and differentiation in vitro. Domestic Animal Endocrinology,2019 106394. Read more>>
  • Yang X et al. miR‐760 exerts an antioncogenic effect in esophageal squamous cell carcinoma by negatively driving fat metabolism via targeting c‐Myc. J Cell Biochem. 2019 Nov 10.Read more>>
  • Wang J et al. Influence of calcium supplementation against fluoride-mediated osteoblasts impairment in vitro: Involvement of canonical Wnt/β-catenin signaling pathway. J Agric Food Chem. 2019 Sep 4. Read more>>
  • Li YH et al. Apple polysaccharide protects ICR mice against colitis associated colorectal cancer through the regulation of microbial dysbiosis. Carbohydrate Polymers 230 (2020) 1157.Read more>>
  • Li Y et al. Apple polysaccharide protects ICR mice against colitis associated colorectal cancer through the regulation of microbial dysbiosis. Carbohydr Polym. 2020 Feb 15;230:115726.Read more>>
  • Yang J et al. MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/β-catenin pathway. Eur J Pharmacol. 2020 Mar 15;871:172927. Read more>>
  • Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
  • Hong Cheet al. circ_0080145 Enhances Imatinib Resistance of Chronic Myeloid Leukemia by Regulating miR-326/PPFIA1 Axis. Cancer Biother Radiopharm. 2020 Jun 27.Read more>>
  • Yang Lv. et al. TGF-1 enhanced myocardial differentiation through inhibition of the Wnt/-catenin pathway with rat BMSCs. Iran J Basic Med Sci. 2020 Aug; 23(8): 1012C1019Read more>>
  • Junmin Li. et al. Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p. Cancer Cell Int. 2021 Dec;21(1):1-15Read more>>
  • Yuyan Wang. et al. ET-1 regulates the human umbilical vein endothelial cell cycle by adjusting the ER/FOXN1 signaling pathway. Ann Transl Med. 2020 Nov; 8(22): 1499Read more>>
  • Hui-Zi Liu. et al. Silencing long non-coding RNA CASC9 inhibits colorectal cancer cell proliferation by acting as a competing endogenous RNA of miR-576-5p to regulate AKT3. Cell Death Discov. 2020 Oct;6(1):1-11Read more>>
  • Zijian Ye. et al. MiR-92b-3p inhibits proliferation and migration of C2C12 cells. Cell Cycle. 2020;19(21):2906-2917Read more>>
  • Enoch Appiah Adu-Gyamfi. et al. Iodothyronine deiodinase 2 (DiO2) regulates trophoblast cell line cycle, invasion and apoptosis; and its downregulation is associated with early recurrent miscarriage. Placenta. 2021 Aug;111:54Read more>>
  • Wu, Guoxian. et al. Circ-RNF111 aggravates the malignancy of gastric cancer through miR-876-3p-dependent regulation of KLF12. World J Surg Oncol. 2021 Dec;19(1):1-12Read more>>
  • Liang Yi. et al. MicroRNA-26b-5p suppresses the proliferation of tongue squamous cell carcinoma via targeting proline rich 11 (PRR11). Bioengineered. 2021;12(1):5830-5838Read more>>
  • Long Zhao. et al. Juglone-loaded metal-organic frameworks for H2O2 self-modulating enhancing chemodynamic therapy against prostate cancer. Chem Eng J. 2022 Feb;430:133057Read more>>
  • Zhang Hailin. et al. MicroRNA-2355-5p Promotes the Proliferation of Head and Neck Squamous Cell Carcinoma via Suppressing NISCH Expression. J Oncol. 2021;2021:2986489Read more>>
  • Que, Tianshi. et al. HMGA1 stimulates MYH9-dependent ubiquitination of GSK-3 via PI3K/Akt/c-Jun signaling to promote malignant progression and chemoresistance in gliomas. Cell Death Dis. 2021 Dec;12(12):1-12Read more>>
  • Peng Gu. et al. Suppression of CDCA3 inhibits prostate cancer progression via NF?B/cyclin D1 signaling inactivation and p21 accumulation. Oncol Rep. 2022 Feb;47(2):1-1Read more>>
VALIDATION IMAGES