Host: Rabbit
Target Protein: Adiponectin receptor 2
Specificity: This antibody may cross react with Adiponectin receptor 1 due to sequence similarity.
Immunogen Range: 315-340/386
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 79602
Swiss Prot: Q86V24
Source: KLH conjugated synthetic peptide derived from human Adiponectin receptor 2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 44
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Liu, Su, et al. "The ameliorating effect of rosiglitazone on experimental nonalcoholic steatohepatitis is associated with regulating adiponectin receptor expression in rats." European journal of pharmacology 650.1 (2011): 384.Read more>>
- Ji, Hongfei, et al. "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." Molecular Biology Reports: 1-9.Read more>>
- Morad, Vivian, Annelie Abrahamsson, and Charlotta Dabrosin. "Estradiol affects extracellular leptin: adiponectin ratio in human breast tissue in vivo." The Journal of Clinical Endocrinology & Metabolism (2014).Read more>>
- Hsu, Ying-Jung, et al. "Effects of Fenofibrate on Adiponectin Expression in Retinas of Streptozotocin-Induced Diabetic Rats." Journal of diabetes research 2014 (2014).Read more>>
- Zhou et al. Associations of adiponectin receptor 2 (AdipoR2) gene polymorphisms and AdipoR2 protein expression levels with the risk of colorectal cancer: A case-control study. (2017) Mol.Med.Rep. 16:3983-3993Read more>>
- Guo et al. Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats. (2012) Cardiovasc.Diabetol. 11:94Read more>>
- Annamria Schaffer. et al. The ontogenies of endometrial and myometrial leptin and adiponectin receptors in pregnant rats: Their putative impact on uterine contractility. Life Sci. 2022 May;297:120465Read more>>
- Guoying Zhu. et al. Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats. HELIYON. 2022 Jul;8:e09872Read more>>
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human rectal tissue labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Heart lysates; Lane 2: Mouse Skeletal muscle lysates; Lane 3: Rat Heart lysates; Lane 4: Rat Skeletal muscle lysates; Lane 5: Human 293T cell lysates probed with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.