bs-0609R [Primary Antibody]
OAT-3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: OAT-3

Immunogen Range: 31-110/536


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9376

Swiss Prot: Q8TCC7

Source: KLH conjugated synthetic peptide derived from human OAT-3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 59


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhou, Qi, et al. "Total saponins from Discorea nipponica makino ameliorate urate excretion in hyperuricemic rats." Pharmacognosy Magazine 11.43 (2015): 567.Read more>>
  • Enoki, Yuki, et al. "Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1." Scientific Reports 6 (2016): 32084.Read more>>
  • Zhu, Liran, et al. "Saponins extracted from Dioscorea collettii rhizomes regulate the expression of urate transporters in chronic hyperuricemia rats." Biomedicine & Pharmacotherapy 93 (2017): 88-94.Read more>>
  • Zhou et al. Total saponins from Discorea nipponica makino ameliorate urate excretion in hyperuricemic rats. (2015) Pharmacogn.Mag. 11:567-73Read more>>
  • Wang Y et al. Chicory (Cichorium intybus L.) inhibits renal reabsorption by regulating expression of urate transporters in fructose-induced hyperuricemia. Journal of Traditional Chinese Medical Sciences. 2019.Read more>>
  • Le Y et al. Anti-Hyperuricemic Effects of Astaxanthin by Regulating Xanthine Oxidase, Adenosine Deaminase and Urate Transporters in RatsMar Drugs.2020 Dec 1;18(12):610.Read more>>
  • Shoma Tanaka. et al. Indoxyl Sulfate Contributes to Adipose Tissue Inflammation through the Activation of NADPH Oxidase. Toxins. 2020 Aug;12(8):502Read more>>
  • Xiaofei Zhou. et al. Coffee Leaf Tea Extracts Improve Hyperuricemia Nephropathy and Its Associated Negative Effect in Gut Microbiota and Amino Acid Metabolism in Rats. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Yong-jun Chen. et al. Salinomycin, a potent inhibitor of XOD and URAT1, ameliorates hyperuricemic nephropathy by activating NRF2, modulating the gut microbiota, and promoting SCFA production. CHEM-BIOL INTERACT. 2024 Aug;:11122Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti OAT-3 Polyclonal Antibody, Unconjugated (bs-0609R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse kidney lysates probed with OAT-3 Polyclonal Antibody, Unconjugated (bs-0609R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.