bs-0608R [Primary Antibody]
ICAM1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ICAM1

Specificity: KO-Validated

Immunogen Range: 201-300/537


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 15894

Swiss Prot: P13597

Source: KLH conjugated synthetic peptide derived from mouse ICAM1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 56


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chu, Changbin, et al. "Celastrol protects kidney against ischemia–reperfusion-induced injury in rats." Journal of Surgical Research (2013).Read more>>
  • Park, Sumin, et al. "Protective effect of 7-O-succinyl macrolactin A against intestinal inflammation is mediated through PI3-kinase/Akt/mTOR and NF-κB signaling pathways." European Journal of Pharmacology (2014).Read more>>
  • Xiong, H., et al. "Glycyrrhizin Ameliorates Imiquimod-Induced Psoriasis-like Skin Lesions in BALB/c Mice and Inhibits TNF-α-Induced ICAM-1 Expression via NF-κB/MAPK in HaCaT Cells." Cellular Physiology and Biochemistry 35.4 (2015): 1335-1346.Read more>>
  • Chenna Narendra S, Chalise JP, Magnusson M, Uppugunduri S (2015) Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis. PLoS ONE 10(10): e0141863Read more>>
  • Tang, Li‐Qin, et al. "The renoprotective effects of berberine and its potential impact on the expression of β‐arrestins and ICAM‐1/VCAM‐1 in streptozocin induced‐diabetic nephropathy rats." Journal of Diabetes (2015).Read more>>
  • Ji, Mingli, et al. "Up-regulation of ICAM-1mRNA and IL-1βmRNA in lung tissues of a rat model of COPD." International Journal of Clinical and Experimental Medicine 8.11 (2016): 21956-21963.Read more>>
  • Sumarya, Made, et al. "BETEL LEAF EXTRACTS (PIPER BETLE L.) PREVENT ENDOTHELIAL DYSFUNCTION BY REDUCING THE LEVELS OF URIC ACID AND ICAM-1 EXPRESSION OF THE HYPERURICEMIA WISTAR RATS (RATTUS NORVEGICUS) AORTIC ENDOTHELIAL." Indonesia Journal of Biomedical ScienceRead more>>
  • Ji, Mingli, et al. "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits."Experimental and Therapeutic Medicine 12.1 (2016): 51-58.Read more>>
  • Zhang, Xiaomei, et al. "Effect of mild hypothermia on breast cancer cells adhesion and migration." Bioscience Trends 6.6 (2012): 313-324.Read more>>
  • Utomo, Pamudji, et al. "Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury." International Journal of ChemTech Research.Read more>>
  • Osada-Oka, Mayuko, et al. "Macrophage-derived exosomes induce inflammatory factors in endothelial cells under hypertensive conditions." Hypertension Research (2016).Read more>>
  • Wang et al. Preliminary Research of Relationship between Acute Peritonitis and Celiac Primo Vessels. (2013) Evid.Based.Complement.Alternat.Me. 2013:569161Read more>>
  • Kim et al. Blocking junctional adhesion molecule C promotes the recovery of cisplatin-induced acute kidney injury. (2017) Korean.J.Intern.Me. 32:1053-1061Read more>>
  • Wang et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin?induced lung inflammation in a murine model of asthma. (2016) Mol.Med.Rep. 13:4289-302Read more>>
  • Tang et al. The renoprotective effects of berberine and its potential impact on the expression of β-arrestins and ICAM-1/VCAM-1 in streptozocin induced-diabetic nephropathy rats. (2015) J.Diabetes. Read more>>
  • Zhang P et al. Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. (2018) Int J Mol Med. 2018 Sep 19. Read more>>
  • Zhang et al. Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. (2018) Int.J.Mol.Med. 42:3344-3354Read more>>
  • Li X et al. Physicochemical properties and laxative effects of polysaccharides from Anemarrhena asphodeloides Bge. In loperamide-induced rats. J Ethnopharmacol. 2019 Aug 10;240:111961. Read more>>
  • Shim W et al. Catechin solubilization by spontaneous hydrogen bonding with poly(ethylene glycol) for dry eyetherapeutics. J Control Release. 2019 May 20.Read more>>
  • Wang Y et al. Tissue Targeting and Ultrasound-Targeted Microbubble Destruction Delivery of Plasmid DNA and Transfection In Vitro. Cellular and Molecular Bioengineering.2019Sep.Read more>>
  • Fang JY et al. Switch of macrophage fusion competency by 3D matrices. Sci Rep. 2020 Jun 25;10(1):10348.Read more>>
  • Qian Liet al. LOX-1 Regulates P. gingivalis-Induced Monocyte Migration and Adhesion to Human Umbilical Vein Endothelial Cells. Front Cell Dev Biol. 2020 Jul 14;8:596.Read more>>
  • Dingkang Xu. et al. Sphingosine\1\phosphate receptor 3 is implicated in BBB injury via the CCL2\CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28Read more>>
  • Wan\Ting Hung. et al. Leptin protects brain from ischemia/reperfusion\induced infarction by stabilizing the bloodCbrain barrier to block brain infiltration by the blood\borne neutrophils. Eur J Neurosci. 2020 Dec;52(12):4890-4907Read more>>
  • Linardi Daniele. et al. Slow versus fast rewarming after hypothermic circulatory arrest: effects on neuroinflammation and cerebral oedema. Eur J Cardio-Thorac. 2020 Oct;58(4):792-8Read more>>
  • Chae Eun Kim. et al. Cevimeline-induced anti-inflammatory effect through upregulations of mucins in the ocular surface of a dry eye mouse model. Biomed Pharmacother. 2021 Jul;139:111571Read more>>
  • Xingyou Hu. et al. Morphology of Biomaterials Affect O-Glycosylation of HUVECs. Journal of Functional Biomaterials. 2022 Dec;13(4):235Read more>>
  • Xiangke Ma. et al. Salvia miltiorrhiza and Tanshinone IIA reduce endothelial inflammation and atherosclerotic plaque formation through inhibiting COX-2. BIOMED PHARMACOTHER. 2023 Nov;167:115501Read more>>
  • Xiaoxiao Zhang. et al. Tanshinone A participates in the treatment of endometriosis by regulating adhesion, invasion, angiogenesis and inhibition of PI3K/Akt/mTOR signaling pathway. MOL MED REP. 2023 Nov;28(5):1-13Read more>>
  • Xiaoyue Guan. et al. The possible role of Gremlin1 in inflammatory apical periodontitis. ARCH ORAL BIOL. 2023 Nov;:105848Read more>>
  • Ay?e Hande Arpaci. et al. Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia?reperfusion injury in sevoflurane?treated rats. MOL MED REP. 2024 Mar;29(3):1-13Read more>>
  • Zeng Wenjie. et al. Preliminary clinical analysis and pathway study of S100A8 as a biomarker for the diagnosis of acute deep vein thrombosis. SCI REP-UK. 2024 Jun;14(1):1-15Read more>>
VALIDATION IMAGES

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54/ICAM-1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 60kD. Observed band size: 60kD.


Image provided by One World Lab validation program. A7R5 cells probed with ICAM1 Polyclonal Antibody (bs-0608R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (bs-0608R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Lane 1: Hela cell lysates; Lane 2: Hela KO ICAM1 cell lysates probed with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Uterine body cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with ICAM1 polyclonal antibody, unconjugated (bs-0608R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.