Host: Rabbit
Target Protein: E2F1
Immunogen Range: 101-180/437
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1869
Swiss Prot: Q01094
Source: KLH conjugated synthetic peptide derived from human E2F1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 46
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Guo, Fangzi, et al. "Endosulfan induces apoptosis by activating the negative regulation pathway of cell cycle and death receptor pathway in spermatogenic cells." Toxicology Research (2017).Read more>>
- Liu, Jianhui, et al. "Silica nanoparticle exposure inducing granulosa cell apoptosis and follicular atresia in female Balb/c mice." Environmental Science and Pollution Research (2017): 1-12.Read more>>
- Sopariwala et al. Long-term PGC1β overexpression leads to apoptosis, autophagy and muscle wasting. (2017) Sci.Re. 7:10237Read more>>
- Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. Read more>>
- Junjie Kong. et al. Silencing of RAB42 down-regulated PD-L1 expression to inhibit the immune escape of hepatocellular carcinoma cells through inhibiting the E2F signaling pathway. CELL SIGNAL. 2023 Apr;:110692Read more>>
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-E2F1 Polyclonal Antibody (bs-0599R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse skeleton lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-0599R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with E2F1 Polyclonal Antibody(bs-0599R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human A673 cell lysates; Lane 3: Human Molt-4 cell lysates; Lane 4: Human U251 cell lysates probed with E2F1 Polyclonal Antibody, Unconjugated (bs-0599R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.