bs-0593R [Primary Antibody]
EpCAM Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EpCAM

Immunogen Range: 53-150/314


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 17075

Swiss Prot: Q99JW5

Source: KLH conjugated synthetic peptide derived from mouse EpCAM

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 35


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiuxiu Wang. et al. Evaluation of the Effects of Different Dietary Patterns on Breast Cancer: Monitoring Circulating Tumor Cells. Foods. 2021 Sep;10(9):2223Read more>>
  • Zengwei Chen. et al. Quantitative analysis of multiple breast cancer biomarkers using DNA-PAINT. ANAL METHODS-UK. 2022 AugRead more>>
  • Zhao Weizhu. et al. Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features. NAT COMMUN. 2023 May;14(1):1-15Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-EpCAM/CD326 Polyclonal Antibody (bs-0593R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rabbit bladder tissue labeled with Rabbit Anti-EpCAM/CD326 Polyclonal Antibody (bs-0593R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rabbit bladder tissue labeled with Rabbit Anti-EpCAM/CD326 Polyclonal Antibody (bs-0593R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.


L1 mouse brain, L2 mouse liver lysates probed (bs-0593R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Observed band size: 32kDa.