bs-0578R
[Primary Antibody]
Pro-Collagen 1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Collagen 1
Specificity: This antibody is produced using a peptide that is found in the C-terminal propeptide region.
Immunogen Range: 1321-1400/1464
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1277
Source: KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Type I collagen levels have been linked to bone growth and formation. Levels of the C-terminal propeptide are indicative of collagen production in vivo. Elevated levels of C-terminal propeptide have been shown in diseases associated with high levels of bone turnover, including Pagets disease, hyperthyroidism, primary hyperparathyroidism and renal osteodystrophy. Slightly elevated levels of C-terminal propeptide have also been documented in some women in early menopause.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 130
Cross Reactive Species:
Human
Mouse
Rat
Dog
Rabbit
Fish
Predicted Cross Reactive Species:
Cow
Sheep
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
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- Xu T et al. MicroRNA\708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.Read more>>
- Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
- Mengmeng Zhang. et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachlorideCinduced liver fibrosis in mice. N-S Arch Pharmacol. 2021 Jan;394(1):189-204Read more>>
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- Yihang Yuet al. Human umbilical cord mesenchymal stem cell attenuates renal fibrosis via TGF-/Smad signaling pathways in vivo and in vitro. Eur J Pharmacol. 2020 Sep 15;883:173343.Read more>>
- Xiajie Linet al. Leucine-activated nanohybrid biofilm for skin regeneration via improving cell affinity and neovascularization capacity. J Mater Chem B. 2020 Sep 21;8(35):7966-7976.Read more>>
- Jian Songet al. 20S-Protopanaxatriol Ameliorates Hepatic Fibrosis, Potentially Involving FXR-Mediated Inflammatory Signaling Cascades. J Agric Food Chem. 2020 Aug 5;68(31):8195-8204.Read more>>
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- Xiaoxiao Zhang. et al. Effect of Guanxin V in animal model of acute myocardial infarction. Bmc Complem Altern M. 2021 Dec;21(1):1-11Read more>>
- Chun-Sheng Shi. et al. Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues. Int J Ophthalmol-Chi. 2021; 14(2): 223C227Read more>>
- Hao H. et al. Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome.. Aging-Us. 2021 Jan;13(2):2982-3009Read more>>
- Chen-Chie Wang. et al. Infrapatellar Fat PadsCDerived Stem Cell Is a Favorable Cell Source for Articular Cartilage Tissue Engineering: An In Vitro and Ex Vivo Study Based on 3D Organized Self-Assembled Biomimetic Scaffold:. Cartilage. 0;()Read more>>
- Jiahui Fu. et al. Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration. Front Bioeng Biotech. 2020; 8: 595096Read more>>
- Maolin Zhang. et al. Recapitulation of cartilage/bone formation using iPSCs via biomimetic 3D rotary culture approach for developmental engineering. Biomaterials. 2020 Nov;260:120334Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-0578R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-0578R) 1:300 followed by conjugation to the secondary PE antibody and DAB staining
Paraformaldehyde-fixed, paraffin-embedded Human Lung Cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Pro-Collagen 1 Polyclonal Antibody, Unconjugated (bs-0578R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Human U-2OS cell lysates probed with Collagen I Polyclonal Antibody, Unconjugated (bs-0578) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Collagen I antibody (bs-0578R) Dilution: 1ug/Test; Protocol Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Collagen I Polyclonal Antibody(bs-0578R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).