bs-0572R-Cy3 [Conjugated Primary Antibody]
CCNB1 Polyclonal Antibody, Cy3 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CCNB1

Immunogen Range: 271-433/433


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 268697

Swiss Prot: P14635

Source: KLH conjugated synthetic peptide derived from human CCNB1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat
Bovine
Chicken

Predicted Cross Reactive Species: Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ghate, Nikhil Baban, et al. "An Antioxidant Extract of Tropical Lichen, Parmotrema reticulatum, Induces Cell Cycle Arrest and Apoptosis in Breast Carcinoma Cell Line MCF-7." PLOS ONE 8.12 (2013): e82293.Read more>>
  • Dai, Hongjuan, et al. "Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest." Tumor Biology (2014): 1-8.Read more>>
  • Zhang, Jihong, et al. "Interleukin 18 augments growth ability via NF-κB and p38/ATF2 pathways by targeting cylin B1, cyclin B2, cylin A2, and Bcl-2 in BRL-3A rat liver cells." Gene (2015).Read more>>
  • Wang, Chengke, and Zhenxin Wang. "Studying the relationship between cell cycle and Alzheimer’s disease by gold nanoparticle probes." Analytical Biochemistry (2015).Read more>>
  • Ghate, N. B., et al. "Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio." Cell Death Discovery 2 (2016).Read more>>
  • Zhang, Jin, et al. "Silica nanoparticles induce start inhibition of meiosis and cell cycle arrest via down-regulating meiotic relevant factors." Toxicology Research (2016).Read more>>
  • Guo, Hongrui, et al. "Dietary NiCl2 causes cell cycle arrest in the broiler's kidney." Oncotarget. (2015) 6.34:35964-77.Read more>>
  • Qu, Zhongyuan, et al. "Chelidonine induces mitotic slippage and apoptotic-like death in SGC-7901 human gastric carcinoma cells." Molecular medicine reports 13.2 (2016): 1336-1344.Read more>>
  • Wang, Qi, et al. "Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies." Food and Chemical Toxicology (2017).Read more>>
  • Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).Read more>>
  • Zeng Q et al.Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice.(2018) Toxicology. (18)30396-2.Read more>>
  • Maziero RRD et al. Effects of the addition of oocyte meiosis-inhibiting drugs on the expression of maturation-promoting factor components and organization of cytoplasmic organelles. Reprod Biol. 2019 Dec 27. pii: S1642-431X(19)30314-6.Read more>>
  • Zhang L et al. Silica Nanoparticles exacerbates reproductive toxicity development in high-fat diet-treated Wistar rats. J Hazard Mater. 2019 Oct 1:121361.Read more>>
  • A K Shendgeet al. A natural flavonoid, apigenin isolated from Clerodendrum viscosum leaves, induces G2/M phase cell cycle arrest and apoptosis in MCF-7 cells through the regulation of p53 and caspase-cascade pathway. Clin Transl Oncol. 2020 Jul 27.Read more>>
  • Anil Khushalrao Shendge. et al. The natural flavones, acacetin and apigenin, induce Cdk-Cyclin mediated G2/M phase arrest and trigger ROS-mediated apoptosis in glioblastoma cells. Mol Biol Rep. 2021 Jan;48(1):539-549Read more>>
  • Zijian Ye. et al. MiR-92b-3p inhibits proliferation and migration of C2C12 cells. Cell Cycle. 2020;19(21):2906-2917Read more>>
  • Hao Zeng. et al. High?throughput sequencing?based analysis of gene expression of hepatitis B virus infection?associated human hepatocellular carcinoma. Oncol Lett. 2020 Oct;20(4):1-1Read more>>
  • Luya Pu. et al. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes. Eur J Pharmacol. 2021 Dec;912:174585Read more>>
  • Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11Read more>>
  • Luchen Sun. et al. Pretreatment of umbilical cord derived MSCs with IFN-_ and TNF-_ enhances the tumor-suppressive effect on acute myeloid leukemia. Biochem Pharmacol. 2022 May;199:115007Read more>>
  • Wang Fang. et al. Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells. BMC CANCER. 2023 Dec;23(1):1-12Read more>>
  • Yunhao Ma. et al. Synthesis and Biological Activity of 2-Chloro-8-methoxy-5-methyl-5H-indolo [2,3-b] Quinoline for the Treatment of Colorectal Cancer by Modulating PI3K/AKT/mTOR Pathways. ACS OMEGA. 2024;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES