bs-0561R [Primary Antibody]
CD62p Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD62p

Immunogen Range: 701-768/768


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 20344

Swiss Prot: Q01102

Source: KLH conjugated synthetic peptide derived from mouse CD62p

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 88


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ma, Yanqin, et al. "Inflammatory responses induced by fluoride and arsenic at toxic concentration in rabbit aorta." Archives of toxicology 86.6 (2012): 849-856.Read more>>
  • Zhou, Birong, et al. "All-trans-retinoic acid ameliorated high fat diet-induced atherosclerosis in rabbits by inhibiting platelet activation and inflammation." BioMed Research International 2012 (2012).Read more>>
  • Rui-kai, Zong, and Liu Jian. "Effects of Xinfeng Capsules on Expression of Platelet Granule Membrane Protein 140 and Platelet Cluster of Differentiation 40 Ligand in Peripheral Blood of Adjuvant Arthritis Rats." International Journal of Rheumatology 2012 (2012).Read more>>
  • Hu, Zhibin, et al. "Comparative analysis of preservation method and intermittent perfusion volume on the expression of endothelial and inflammatory markers by coronal artery and myocardium in porcine donor hearts." ASAIO journal (American Society for Artificial Internal Organs: 1992) (2014).Read more>>
  • Song, Shang, et al. "An intravascular bioartificial pancreas device (iBAP) with silicon nanopore membranes (SNM) for islet encapsulation under convective mass transport." Lab on a Chip 17.10 (2017): 1778-1792.Read more>>
  • Rui-Kai and Jian Effects of xinfeng capsules on expression of platelet granule membrane protein 140 and platelet cluster of differentiation 40 ligand in peripheral blood of adjuvant arthritis rats. (2012) Int.J.Rheumato. 2012:139696Read more>>
  • Pan et al. Effects of Local Pancreatic Renin-Angiotensin System on the Microcirculation of Rat with Severe Acute Pancreatitis. (2015) Korean.J.Physiol.Pharmacol. 19:299-307Read more>>
  • Song et al. An intravascular bioartificial pancreas device (iBAP) with silicon nanopore membranes (SNM) for islet encapsulation under convective mass transport. (2017) Lab.Chip. 17:1778-1792Read more>>
  • inggang Wu. et al. Extracellular matrix coating improves the biocompatibility of polymeric heart valves. J Mater Chem B. 2020 Dec;8(46):10616-10629Read more>>
  • Qi-Rui Li. et al. Platelets are highly efficient and efficacious carriers for tumor-targeted nano-drug delivery. Drug Deliv. 2022;29(1):937-949Read more>>
  • Haifeng Gong. et al. Platelet shipped IL-10 enhances drug delivery for attenuating I/R- or UUO-induced renal injury. CHEM ENG J. 2023 Apr;462:142258Read more>>
  • Stanley M. H. Chan. et al. Inhibition of oxidative stress by apocynin attenuated chronic obstructive pulmonary disease progression and vascular injury by cigarette smoke exposure. BRIT J PHARMACOL. 2023 MaRead more>>
  • Tian-Qi Ma. et al. Indocyanine green-loaded platelet activated by photodynamic and photothermal effects for selective control of wound repair. PHOTODIAGN PHOTODYN. 2024 Feb;45:103945Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti-CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-P-selectin Polyclonal Antibody (bs-0561R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.


Lane 1: K562 cell lysates; Lane 2: Huvec cell lysates; Lane 3: Jurkat cell lysates probed with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.