bs-0559R [Primary Antibody]
CDK5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CDK5

Specificity: This antibody will detect both isoform variants of this protein. Due to similar amino acid composition of this protein to CDK3 in human, there is a chance that this antibody will react with CDK3 in human, based on an 83% sequence similarity.

Immunogen Range: 15-100/292


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1020

Swiss Prot: Q00535

Source: KLH conjugated synthetic peptide derived from human CDK5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

CDK5 is serine/threonine kinase involved in synaptic regulation and neuronal development; phosphorylates synaptic protein Pctaire1; regulates acetylcholine receptor expression.CDK5 is a member of the cyclindependent kinase family of serine/threonine kinases. It is present in numerous mammalian tissues including kidney, testes, and ovary. Its activity is detected almost exclusively in brain extracts. Neuronal and muscle cells contain the highest amount of this protein. Similar to other Cdks, monomeric Cdk5 displays no enzymatic activity, but Cdk5 is not activated by cyclins. Instead, the p35 protein, which is expressed solely in the brain, activates Cdk5. Cdk5 interacts with D1 and D3 type G1 cyclins and can phosphorylate histone H1, TAU, MAP2 and NF-H and NF-M. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yin, Xiang, et al. "Roscovitine treatment caused impairment of fertilizing ability in mice." Toxicology Letters (2015).Read more>>
  • Li, Shuang, et al. "Protective effect of Xingnaojia formulation on rats with brain and liver damage caused by chronic alcoholism." Experimental and Therapeutic Medicine 10.5 (2015): 1643-1652.Read more>>
  • Cao, Yu, et al. "Traditional Chinese Medicine Huannao Yicong Decoction Extract Decreases Tau Hyperphosphorylation in the Brain of Alzheimer’s Disease Model Rats Induced by Aβ1–42." Evidence-Based Complementary and Alternative Medicine 2016 (2016).Read more>>
  • Wei Zhang. et al. FoxO1 overexpression reduces A production and tau phosphorylation in vitro. Neurosci Lett. 2020 Nov;738:135322Read more>>
  • Yang, Nanfei. et al. Blockage of PPAR T166 phosphorylation enhances the inducibility of beige adipocytes and improves metabolic dysfunctions. CELL DEATH DIFFER. 2022 Nov;:1-13Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDK5 Polyclonal Antibody, Unconjugated(bs-0559R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-CDK5 Polyclonal Antibody (bs-0559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Mouse Large intestine lysates; Lane 2: Rat Large intestine lysates probed with CDK5 Polyclonal Antibody, Unconjugated (bs-0559R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.