Host: Rabbit
Target Protein: Collagen 5
Specificity: This antibody reacts with Collagen V but also exhibits slightly with Collagen IV
Immunogen Range: 1450-1500/1838
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1289
Swiss Prot: P20908
Source: KLH conjugated synthetic peptide derived from human Collagen V
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 184
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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Formalin-fixed and paraffin-embedded rat skin tissue labeled with Anti-Collagen V Polyclonal Antibody, Unconjugated (bs-0552R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Anti-Collagen V Polyclonal Antibody, Unconjugated (bs-0552R) 1:200 followed by conjugation to the secondary PE antibody and DAB staining
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Skin tissue lysates; Lane 3: Mouse Uterus tissue lysates; Lane 4: Rat Uterus tissue lysates; Lane 5: Human A549 cell lysates probed with COL5A1 Polyclonal Antibody, Unconjugated (bs-0552R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.