bs-0545R
[Primary Antibody]
SCF Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: SCF
Immunogen Range: 155-209/273
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4254
Swiss Prot: P21583
Source: KLH conjugated synthetic peptide derived from human SCF
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 31
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Goldstein, Bradley J., et al. "Adult c‐Kit (+) progenitor cells are necessary for maintenance and regeneration of olfactory neurons." Journal of Comparative Neurology (2014).Read more>>
- Wang, Li, et al. "Silencing stem cell factor attenuates stemness and inhibits migration of cancer stem cells derived from Lewis lung carcinoma cells."Tumor Biology (2015): 1-15.Read more>>
- Lin, Xue-Lian, et al. "NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF." PloS one 11.2 (2016): e0149031.Read more>>
- Yin et al. Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation. (2018) Int.J.Mol.Med. 41:649-658Read more>>
- Choo E et al. The c-kit Receptor Tyrosine Kinase Marks Sweet or Umami Sensing T1R3 Positive Adult Taste Cells in Mice. Chemosensory Perception. 2020.Read more>>
- Xi Huaming. et al. Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season. Biol Reprod. 2021 AugRead more>>
- Huaming Xi. et al. FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells. Mol Cell Endocrinol. 2021 Nov;:111505Read more>>
- Hongjun Kuang. et al. Electroacupuncture Improves Intestinal Motility through Exosomal miR-34c-5p Targeting SCF/c-Kit Signaling Pathway in Slow Transit Constipation Model Rats.. EVID-BASED COMPL ALT. 2022 Sep;2022:8043841-8043841Read more>>
- Huaming Xi. et al. FSH-inhibited autophagy protects against oxidative stress in goat Sertoli cells through p62-Nrf2 pathway. THERIOGENOLOGY. 2023 Jan;195:103Read more>>
- Ozge Goktepe. et al. The effect of different doses of nonylphenol on the blood-testicular barrier integrity, hormone level, and DNA damage in the testes of rats. FOOD CHEM TOXICOL. 2023 Jul;177:113816Read more>>
- Di Zhang. et al. Effects of Banxia Xiexin Decoction on apoptosis of interstitial cells of Cajal via regulation of MiR-451-5p: An in vivo and in vitro study. J ETHNOPHARMACOL. 2023 Oct;314:116606Read more>>
- Karaman Enes. et al. Protective Effects of Boric Acid Taken in Different Ways on Experimental Ovarian ?schemia and Reperfusion. BIOL TRACE ELEM RES. 2023 Sep;:1-14Read more>>
- Erol Suleyman. et al. In vitro evaluation of exocytosis-associated SNARE molecules in human granulosa cells in polycystic ovary syndrome. J ASSIST REPROD GEN. 2023 Nov;:1-13Read more>>
- Lei Wu. et al. Naringenin Promotes Gastrointestinal Motility in Mice by Impacting the SCF/c-Kit Pathway and Gut Microbiota. FOODS. 2024 Jan;13(16):252Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-SCF Polyclonal Antibody (bs-0545R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:33 for 30 min at room temperature. A Goat anti-rabbit IgG-AF647 secondary antibody was used at 1ug for 40 min at room temperature. Acquisition of 10,000 events was performed. Primary antibody staining (green) is compared to compared to unstained cells (purple), secondary only (light blue), and isotype control (orange).
Sp2/0 (Mouse myeloma cell) lysates probed with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U87MG cells were fixed with 4% PFA (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:100 for 30 min at room temperature. A Goat anti-rabbit IgG-PE (bs-0295G-PE) secondary antibody was used at 1ug for 40 min at room temperature. Acquisition of 20,000 events was performed. Primary antibody staining (green) is compared to compared to unstained cells (purple), secondary only (light blue), and isotype control (orange).
Paraformaldehyde-fixed, paraffin-embedded Rat Spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SCF Polyclonal Antibody(bs-0545R-AF647)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates; Lane 3:Human MCF-7 cell lysates; Lane 4: Human Hela cell lysates probed with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at -20℃ for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SCF) polyclonal Antibody, Unconjugated (bs-0545R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.