bs-0539R [Primary Antibody]
Chromogranin A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Chromogranin A

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1113

Swiss Prot: P10645

Source: KLH conjugated synthetic peptide derived from human CGA

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Yuli, et al. "In vitro generation of colonic epithelium from primary cells guided by microstructures." Lab on a Chip (2014).Read more>>
  • Liu, Yanchun, et al. "Enriched Intestinal Stem Cell Seeding Improves the Architecture of Tissue Engineered Intestine." Tissue Engineering (2014).Read more>>
  • Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016).Read more>>
  • Wang, Yuli, et al. "Capture and 3D culture of colonic crypts and colonoids in a microarray platform." Lab on a Chip 13.23 (2013): 4625-4634.Read more>>
  • Liu, Yanchun, et al. "HB-EGF Embedded in PGA/PLLA Scaffolds via Subcritical CO 2 Augments the Production of Tissue Engineered Intestine."Biomaterials (2016).Read more>>
  • Wang et al. In vitro generation of colonic epithelium from primary cells guided by microstructures. (2014) Lab.Chi. 14:1622-31Read more>>
  • Shuang Xie. et al. Indispensable role of melatonin, a scavenger of reactive oxygen species (ROS), in the protective effect of Akkermansia muciniphila in cadmium-induced intestinal mucosal damage. FREE RADICAL BIO MED. 2022 Nov;193:447Read more>>
  • Geng-xiu Zan. et al. Heat stress disrupts intestinal stem cell migration and differentiation along the cryptCvillus axis through FAK signaling. BBA-MOL CELL RES. 2023 Mar;1870:119431Read more>>
  • Ko-Chao Lee. et al. Use of iTRAQ-based quantitative proteomic identification of CHGA and UCHL1 correlated with lymph node metastasis in colorectal carcinoma. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 MayRead more>>
  • Kang Tae Hong. et al. Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function. J ANIM SCI BIOTECHNO. 2024 Dec;15(1):1-12Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-CGA/ Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Chromogranin A Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 overnight at 4°C, DAB staining.