bs-0530R [Primary Antibody]
PPAR gamma Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PPAR gamma

Immunogen Range: 101-200/505


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5468

Swiss Prot: P37231

Source: KLH conjugated synthetic peptide derived from human PPAR Gamma

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The PPAR gamma antibody mainly is exist in the white fat organization, the fat for the PPAR gamma is born, blood sugar stability, the disease respond, the artery gruel kind hardens to rise the important function with the tumor occurrence of etc. all, but concerning the PPAR gamma to bone of function is a new research heat to order in recent years.A PPAR of many researches report gamma was go together with the body is after activate can the function promote many capable cells divided to increase to living but repress the ossification cell to divide to cause the bone measure the decrease or bone softs toward the fat cell in the marrow, the PPAR gamma promotes the ability and bones that the fat cell divide metabolize closely related, the performance is increasing along with the growth marrow fat content of the age, the ossification cell metabolism the outcome reduce, the different construction PPAR gamma 2 have the important function.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 57


Cross Reactive Species: Human
Mouse
Rat
Bovine
Sheep

Predicted Cross Reactive Species: Cow
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang, Wucai, et al. "Melatonin promotes triacylglycerol accumulation via MT2 receptor during differentiation in bovine intramuscular preadipocytes." Scientific Reports 7.1 (2017): 15080.Read more>>
  • Zhang et al. 1,25-Dihydroxyvitamin D₃ Promotes High Glucose-Induced M1 Macrophage Switching to M2 via the VDR-PPARγ Signaling Pathway. (2015) Biomed.Res.In. 2015:157834Read more>>
  • Zhang et al. Correlation between TSP-1, TGF-β and PPAR-γ expression levels and glioma microvascular density. (2014) Oncol.Let. 7:95-100Read more>>
  • Yao et al. Wogonin prevents lipopolysaccharide-induced acute lung injury and inflammation in mice via peroxisome proliferator-activated receptor gamma-mediated attenuation of the nuclear factor-kappaB pathway. (2014) Immunology. 143:241-57Read more>>
  • Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137Read more>>
  • Manabe A et al.Upregulation of transient receptor potential melastatin 6 channel expression by rosiglitazone and all-trans-retinoic acid in erlotinib-treated renal tubular epithelial cells.(2018)J Cell Physiol. Read more>>
  • Wang F et al. Insulin‑like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas. Mol Med Rep. 2019 Jan 24. Read more>>
  • Dong S et al. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sci. 2019 Jun 15;227:20-29. Read more>>
  • Li B et al. Resistin up-regulates LPL expression through the PPARγ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264. 7 macrophages.Cytokine. 2019 Jul;119:168-174.Read more>>
  • Zhang K et al. Insulin‑like growth factor 2 promotes the adipogenesis of hemangioma‑derived stem cells. Exp Ther Med. 2019 Mar;17(3):1663-1669. Read more>>
  • Min Zhao et al. HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway. Mediators of Inflammation, 2019, 1–17. Read more>>
  • "Wang L et al. Antiobesity, Regulation of Lipid Metabolism, and Attenuation of Liver Oxidative Stress Effects of Hydroxy-α-sanshool Isolated from Zanthoxylum bungeanum on High-Fat Diet-Induced Hyperlipidemic Rats. Oxidative Medicine and Cellular Longevity. 2019 ID 5852494."Read more>>
  • Xu HY et al. Bovine bta-microRNA-1271 Promotes Preadipocyte Differentiation by Targeting Activation Transcription Factor 3. Biochemistry (Mosc). 2020 Jul;85(7):749-757.Read more>>
  • Kai Kanget al. FGF21 attenuates neurodegeneration through modulating neuroinflammation and oxidant-stress. Biomed Pharmacother. 2020 Sep;129:110439.Read more>>
  • Ming Qiet al. Postnatal growth retardation is associated with intestinal mucosa mitochondrial dysfunction and aberrant energy status in piglets. J Cell Mol Med. 2020 Sep;24(17):10100-10111.Read more>>
  • Chao X et al. miR-429-3p/LPIN1 Axis Promotes Chicken Abdominal Fat Deposition via PPAR PathwayFront Cell Dev Biol.2020 Dec 21;8:595637.Read more>>
  • Haodong Liu. et al. miR\340\5p inhibits sheep adipocyte differentiation by targeting ATF7. Anim Sci J. 2020 Jan;91(1):e13462Read more>>
  • Yaxin Zhang. et al. Dingxin Recipe IV attenuates atherosclerosis by regulating lipid metabolism through LXR-/SREBP1 pathway and modulating the gut microbiota in ApoE-/- mice fed with HFD. J Ethnopharmacol. 2021 Feb;266:113436Read more>>
  • Haizhao Zhao. et al. The Influence of Bosentan on MicroRNA-27a/PPAR/ET-1 Signaling Pathway in Pulmonary Artery Hypertension. 2021 Apr 15Read more>>
  • Lijin Guo. et al. Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/FNIP2 Axis in Chicken Adipogenesis. Front Cell Dev Biol. 2021; 9: 653798Read more>>
  • Lei Ma. et al. Identification of the anti-fungal drug fenticonazole nitrate as a novel PPAR-modulating ligand with good therapeutic index: Structure-based screening and biological validation. Pharmacol Res. 2021 Nov;173:10586Read more>>
  • Tian, Mengyue. et al. The protective effect of licochalcone A against inflammation injury of primary dairy cow claw dermal cells induced by lipopolysaccharide. Sci Rep-Uk. 2022 Jan;12(1):1-9Read more>>
  • Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1_ and _-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16Read more>>
  • Yao Yao. et al. Short-chain fatty acids regulate B cells differentiation via FFAR2 to alleviate rheumatoid arthritis. BRIT J PHARMACOL. 2022 Apr 07Read more>>
  • Songsong Jiang. et al. A Comparison Study on the Therapeutic Effect of High Protein Diets Based on Pork Protein versus Soybean Protein on Obese Mice. FOODS. 2022 Jan;11(9):1227Read more>>
  • Yue-Qiang Huang. et al. Di-2-ethylhexyl phthalate (DEHP) induced lipid metabolism disorder in liver via activating the LXR/SREBP-1c/PPAR_/_ and NF-_B signaling pathway. FOOD CHEM TOXICOL. 2022 Jul;165:113119Read more>>
  • Zhen Zhou. et al. Myogenic Determination and Differentiation of Chicken Bone Marrow-Derived Mesenchymal Stem Cells under Different Inductive Agents. ANIMALS. 2022 Jan;12(12):1531Read more>>
  • Lijin Guo. et al. Chicken Protein S Gene Regulates Adipogenesis and Affects Abdominal Fat Deposition. ANIMALS. 2022 Jan;12(16):2046Read more>>
  • Fangyuan Chen. et al. Identification of a novel PPAR modulator with good anti-diabetic therapeutic index via structure-based screening, optimization and biological validation. BIOMED PHARMACOTHER. 2022 Oct;154:113653Read more>>
  • Yongli Han. et al. Distribution characteristics and regulation of amino acids and fatty acids in muscle and adipose tissues of sheep grown in natural grazing environment. ANIM SCI J. 2022 Sep;93(1):e13769Read more>>
  • Junyuan Tang. et al. Structure-based screening and biological validation of the anti-thrombotic drug-dicoumarol as a novel and potent PPAR-modulating ligand. BIOORG CHEM. 2022 Oct;:106191Read more>>
  • Xiaoyu Qu. et al. Integration of metabolomics and proteomics analysis to explore the mechanism of neurotoxicity induced by receipt of isoniazid and rifampicin in mice. NEUROTOXICOLOGY. 2023 Jan;94:24Read more>>
  • Kaili Cui. et al. Ferulic Acid and P-Coumaric Acid Synergistically Attenuate Non-Alcoholic Fatty Liver Disease through HDAC1/PPARG-Mediated Free Fatty Acid Uptake. INT J MOL SCI. 2022 Jan;23(23):15297Read more>>
  • Li Kan. et al. m6A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte. Journal of Animal Science and Biotechnology. 2022 Dec;13(1):1-15Read more>>
  • Man Luo. et al. miR136 regulates proliferation and differentiation of small tail han sheep preadipocytes. ADIPOCYTE. 2023;12(1):Article: 2173966Read more>>
  • Jing Fan. et al. Syndecan-3 Coregulates Milk Fat Metabolism and Inflammatory Reactions in Bovine Mammary Epithelial Cells through AMPK/SIRT1 Signaling Pathway. INT J MOL SCI. 2023 Jan;24(7):6657Read more>>
  • Liu Jiayi. et al. Lithium Chloride Promotes Endogenous Synthesis of CLA in Bovine Mammary Epithelial Cells. BIOL TRACE ELEM RES. 2023 Apr;:1-14Read more>>
  • Qicai Liu. et al. Adropin deficiency worsens TNBS-induced colitis. INT IMMUNOPHARMACOL. 2023 Nov;124:110891Read more>>
  • Yanghuan Yu. et al. MiRNA-seq and mRNA-seq revealed the mechanism of fluoride-induced cauda epididymal injury. SCI TOTAL ENVIRON. 2024 Jun;930:172895Read more>>
  • Chao Xiaohuan. et al. ALKBH5 regulates chicken adipogenesis by mediating LCAT mRNA stability depending on m6A modification. BMC GENOMICS. 2024 Dec;25(1):1-14Read more>>
  • Lei Ma. et al. Structure-based screening, optimization and biological evaluation of novel chrysin-based derivatives as selective PPAR modulators for the treatment of T2DM and hepatic steatosis. EUR J MED CHEM. 2024 Oct;276:116728Read more>>
  • Jingjing Chen. et al. Amelioration of Oxidative Stress in Rats with Chronic Obstructive Pulmonary Disease through Shenqi Huatan Decoction Activation of Peroxisome Proliferator-Activated Receptor Gamma-Mediated Activated Protein Kinase/Forkhead Transcription Factor O3a Signaling Pathway. CAN RESPIR J. 2024 Jul;2024(1):5647813Read more>>
  • Yu Wenjing. et al. Bergenin mitigates neuroinflammatory damage induced by high glucose: insights from Zebrafish, murine microbial cell line, and rat models. FRONT PHARMACOL. 2024 Aug;15Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded human skin psoriasis labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse stomach lysates probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Sheep heart lysates probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


293T Cells probed with PPAR gamma Polyclonal Antibody, unconjugated (bs-0530R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C.


Formalin-fixed and paraffin-embedded Mouse lung tissue labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


U251 cells fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells were incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions and then probed with rabbit anti-PPARG Polyclonal Antibody, Unconjugated (bs-0530R) (green) at 1:100 for 30 minutes at room temperature, followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).


Lane 1: Mouse Liver lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Lung lysates probed with PPAR gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PPAR gamma) polyclonal Antibody, Unconjugated (bs-0530R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: Human A549 cell lysates; Lane 2: Rat Adipocyte cell lysates; Lane 3: Mouse Large intestine lysates; Lane 4: Mouse Lung lysates probed with PPAR Polyclonal Antibody, Unconjugated (bs-0530R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Breast tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Rat Adipose tissue lysates; Lane 4: Rat Breast tissue lysates; Lane 5: Rat Lung tissue lysates; Lane 6: Human A431 cell lysates; Lane 7: Human A549 cell lysates; Lane 8: Human THP-1 cell lysates; Lane 9: Human 293T cell lysates probed with PPAR gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.