bs-0521R-FITC [Conjugated Primary Antibody]
CD44 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD44

Immunogen Range: 701-742/742


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 960

Swiss Prot: P16070

Source: KLH conjugated synthetic peptide derived from human CD44

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 85


Cross Reactive Species: Human
Mouse
Rat
Rabbit
Others

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Xuming, et al. "Characterization of sphere‑forming cells with stem‑like properties from the gastric cancer cell lines MKN45 and SGC7901." Molecular Medicine Reports 10.6 (2014): 2937-2941.Read more>>
  • Zhu et al. Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells. (2017) Mol.Med.Re. 16:3201-3207Read more>>
  • Wu et al. Subchondral bone changes and chondrogenic capacity of progenitor cells from subchondral bone in the collagenase-induced temporomandibular joints osteoarthritis rabbit model. (2015) Int.J.Clin.Exp.Patho. 8:9782-9Read more>>
  • Neal et al. The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor. (2018) J.Neuroinflammation. 15:73Read more>>
  • Xu et al. NIBP impacts on the expression of E-cadherin, CD44 and vimentin in colon cancer via the NF-κB pathway. (2016) Mol.Med.Rep. 13:5379-85Read more>>
  • Liao et al. Bone mesenchymal stem cells co-expressing VEGF and BMP-6 genes to combat avascular necrosis of the femoral head. (2018) Exp.Ther.Med. 15:954-962Read more>>
  • Xiong et al. Bone Marrow Mesenchymal Stem-Cell Transplantation Promotes Functional Improvement Associated with CNTF-STAT3 Activation after Hemi-Sectioned Spinal Cord Injury in Tree Shrews. (2017) Front.Cell.Neurosci. 11:172Read more>>
  • Zhang et al. Comparisons of rabbit bone marrow mesenchymal stem cell isolation and culture methods in vitro. (2014) PLoS.One. 9:e88794Read more>>
  • "Chen F et al. The biological characteristics of sheep umbilical cord mesenchymal stem cells.Can J Vet Res. 2018 Jul;82(3):216-224."Read more>>
  • Kaiyuan Wanget al. An exosome-like programmable-bioactivating paclitaxel prodrug nanoplatform for enhanced breast cancer metastasis inhibition. Biomaterials. 2020 Oct;257:120224.Read more>>
  • Xue Yanget al. Star miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer. J Control Release. 2020 Oct 10;326:615-627.Read more>>
  • Xiaohui Chen. et al. Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-497-5p inhibit RSPO2 and accelerate OPLL. Life Sci. 2021 Apr;:119481Read more>>
  • Yue Wu. et al. LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis. Cancer Cell Int. 2021 Dec;21(1):1-14Read more>>
  • Xuejun Dai. et al. Comparison of the differentiation abilities of bone marrow?derived mesenchymal stem cells and adipose?derived mesenchymal stem cells toward nucleus pulposus?like cells in three?dimensional culture. Exp Ther Med. 2021 Sep;22(3):1-9Read more>>
  • Zhao, Gang. et al. LINC02381, a sponge of miR-21, weakens osteogenic differentiation of hUC-MSCs through KLF12-mediated Wnt4 transcriptional repression. 2021 Nov 15Read more>>
  • Xin Zhou. et al. Tumour-derived extracellular vesicle membrane hybrid lipid nanovesicles enhance siRNA delivery by tumour-homing and intracellular freeway transportation. J Extracell Vesicles. 2022 Mar;11(3):e12198Read more>>
  • Guo, Yuxin. et al. In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses. NAT COMMUN. 2022 Nov;13(1):1-2Read more>>
  • Qing Li. et al. SAMD9 Promotes Postoperative Recurrence of Esophageal Squamous Cell Carcinoma by Stimulating MYH9-Mediated GSK3/-Catenin Signaling. Advanced Science. 2023 Feb;:2203573Read more>>
  • Guanghui Li. et al. Lipid Regulatory Element Interact with CD44 on Mitochondrial Bioenergetics in Bovine Adipocyte Differentiation and Lipometabolism. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES

Raw264.7 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD44 Polyclonal Antibody（bs-0521R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).