bs-0520R-PE [Conjugated Primary Antibody]
PKA alpha + beta Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PKA alpha + beta

Immunogen Range: 17-100/351


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 282323

Swiss Prot: P05131

Source: KLH conjugated synthetic peptide derived from bovine PKA

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, and differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (By similarity).

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 40


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Haolong, Du, et al. "Enterovirus 71 VP1 Activates Calmodulin-Dependent Protein Kinase II and Results in the Rearrangement of Vimentin in Human Astrocyte Cells." PLoS One 8(9): e73900Read more>>
  • Yao, Gaoyi, et al. "Chronic SO 2 inhalation above environmental standard impairs neuronal behavior and represses glutamate receptor gene expression and memory-related kinase activation via neuroinflammation in rats." Environmental Research 137 (2015): 85-93.Read more>>
  • Zhou, Zhiwen, et al. "Photoactivated adenylyl cyclase (PAC) reveals novel mechanisms underlying cAMP-dependent axonal morphogenesis." Scientific Reports 5 (2016): 19679.Read more>>
  • Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
  • Yang Liuet al. Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway. Exp Ther Med. 2020 Jul;20(1):495-499.Read more>>
  • Yongxiang Li. et al. Food reward depends on TLR4 activation in dopaminergic neurons. Pharmacol Res. 2021 Jul;169:105659Read more>>
  • Tong Hongxuan. et al. Molecular Mechanism of Tetramethylpyrazine Ameliorating Neuroexcitotoxicity through Activating the PKA/CREB Signaling Pathway. Biomed Res Int. 2022;2022:2812839Read more>>
  • Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292Read more>>
  • Zhongxin Li. et al. Duodenal infusion of soy protein hydrolysate activates cAMP signaling and hypothalamic neurotransmitter synthesis in pigs. FOOD FUNCT. 2022 NovRead more>>
  • Shiqing Sun. et al. Pharmacodynamic structure of deer antler base protein and its mammary gland hyperplasia inhibition mechanism by mediating Raf-1/MEK/ERK signaling pathway activation. FOOD FUNCT. 2023 MaRead more>>
  • Jiajun Chen. et al. Integrating UHPLC-MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism. J PHARM ANAL. 2023 AugRead more>>
  • Yanfei Shi. et al. Naringenin promotes the expression of oxidized myofibers via the PKA signaling pathway in C57BL/6J mice and C2C12 cells. J FUNCT FOODS. 2023 Dec;111:105902Read more>>
  • Liu Chunxiao. et al. Targeting P2Y14R protects against necroptosis of intestinal epithelial cells through PKA/CREB/RIPK1 axis in ulcerative colitis. NAT COMMUN. 2024 Mar;15(1):1-16Read more>>
  • Huinan Wang. et al. Formation mechanism, prevention of malignant ascites effusion and reduction of intestinal mucosal irritation of natural microemulsion from Euphorbia lathyris Pulveratum. BIOMED PHARMACOTHER. 2024 Sep;178:117253Read more>>
  • Qinbing Xue. et al. Lycorine (Lycoris radiata)a unique natural medicine on breast cancer. J CELL MOL MED. 2024 Aug;28(16):e70032Read more>>
VALIDATION IMAGES