bs-0512R [Primary Antibody]
CD2AP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD2AP

Immunogen Range: 561-639/639


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23607

Swiss Prot: Q9Y5K6

Source: KLH conjugated synthetic peptide derived from human CD2AP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 71


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xinying Zhang. et al. Exogenous spermine attenuates diabetic kidney injury in?rats by inhibiting AMPK/mTOR signaling pathway. Int J Mol Med. 2021 Mar;47(3):1-1Read more>>
  • Derya Karabulut. et al. A different perspective on the filtration barrier after kidney stone formation: An immunohistochemical and biochemical study. 2020 Nov 05Read more>>
  • Lifeng Wei. et al. Shenqi granule upregulates CD2AP and -actinin4 and activates autophagy through regulation of mTOR/ULK1 pathway in MPC5 cells. J ETHNOPHARMACOL. 2023 Mar;303:115942Read more>>
  • Zeng Xiaoshan. et al. Hypoxia-inducible factor-1 attenuates renal podocyte injury in male rats in a simulated high-altitude environment by upregulating Krppel-like factor 4 expression. EXP PHYSIOL. 2024 MayRead more>>
  • J.I. Yue. et al. Acupuncture improve proteinuria in diabetic kidney disease rats by inhibiting ferroptosis and epithelial-mesenchymal transition. HELIYON. 2024 Jul;1Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-CD2AP Polyclonal Antibody (bs-0512R) , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with CD2AP Polyclonal Antibody, Unconjugated (bs-0512R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.