bs-0489R [Primary Antibody]
BrdU Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BrdU

Clonality: Polyclonal

Isotype: IgG

Source: BrdU conjugated to KLH

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 0.3071


Cross Reactive Species: Species independent
(BrdU)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Rui, et al. "Neuroprotective Effects of Sulforaphane on Cholinergic Neurons in Mice with Alzheimer’s Disease-Like Lesions." International Journal of Molecular Sciences 15.8 (2014): 14396-14410.Read more>>
  • Giuliani, Daniela, et al. "NDP-α-MSH induces intense neurogenesis and cognitive recovery in Alzheimer transgenic mice through activation of melanocortin MC 4 receptors." Molecular and Cellular Neuroscience (2015).Read more>>
  • Chen et al. Sodium iodate influences the apoptosis, proliferation and differentiation potential of radial glial cells in vitro. (2014) Cell.Physiol.Bioche. 34:1109-24Read more>>
  • Zhang et al. Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice. (2015) World.J.Gastroenterol. 21:4195-209Read more>>
  • Jian Zhang. et al. Collagen/heparan sulfate porous scaffolds loaded with neural stem cells improve neurological function in a rat model of traumatic brain injury. Neural Regen Res. 2021 Jun;16(6):1068Read more>>
  • Victoria Gudi?o. et al. RAC1B modulates intestinal tumourigenesis via modulation of WNT and EGFR signalling pathways. Nat Commun. 2021 Apr;12(1):1-17Read more>>
  • Yuya Higashi. et al. Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray. Sci Rep-Uk. 2021 Jul;11(1):1-12Read more>>
  • Shiyun Chen. et al. Targeted delivery of Chinese herb pair-based berberine/tannin acid self-assemblies for the treatment of ulcerative colitis. J Adv Res. 2021 NovRead more>>
  • Hao Wu. et al. Nattokinase Promotes Post-stroke Neurogenesis and Cognition Recovery via Increasing Circulating Irisin. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES

Image provided by One World Lab validation program. Hela(top) and MCF-7(bottom) cells were labeled with BrdU (10μM) and stained with Rabbit Anti-BrdU Polyclonal Antibody (bs-0489R) at 1:100 dilution. 2nd antibody without primary antibody was used as control included. Fluorescent signals were detected with primary antibody dilution in both Hela and MCF-7.


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Brdu Polyclonal Antibody (bs-0489R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining