bs-0467R [Primary Antibody]
Ghrelin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Ghrelin

Immunogen Range: 31-117/117


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51738

Swiss Prot: Q9UBU3

Source: KLH conjugated synthetic peptide derived from human Ghrelin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 3-Dec


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lemarié F, Beauchamp E, Dayot S, Duby C, Legrand P, Rioux V (2015) Dietary Caprylic Acid (C8:0) Does Not Increase Plasma Acylated Ghrelin but Decreases Plasma Unacylated Ghrelin in the Rat. PLoS ONE 10(7): e0133600.Read more>>
  • Dai, Meiling, et al. "Effects of zearalenone-diet on expression of ghrelin and PCNA genes in ovaries of post-weaning piglets." Animal Reproduction Science (2016).Read more>>
  • Wölnerhanssen, Bettina K., et al. "Deregulation of transcription factors controlling intestinal epithelial cell differentiation; a predisposing factor for reduced enteroendocrine cell number in morbidly obese individuals." Scientific Reports 7 (2017).Read more>>
  • Wang et al. Effects of Wen Dan Tang on insomnia-related anxiety and levels of the brain-gut peptide Ghrelin. (2014) Neural.Regen.Re. 9:205-12Read more>>
  • Liu Y et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. Int. J. Mol. Med. Sep 18Read more>>
  • Liu et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. (2018) Int.J.Mol.Med. 42:3037-3046Read more>>
  • Quanwei Zhang. et al. Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts. Toxins. 2021 Oct;13(10):736Read more>>
  • Quancheng Liu. et al. FumDSB Can Reduce the Toxic Effects of Fumonisin B1 by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs. Toxins. 2021 Dec;13(12):874Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Ghrelin Polyclonal Antibody (bs-0467R), Unconjugated at 1:100, followed by conjugation to the secondary antibody and DAB staining