bs-0465R
[Primary Antibody]
NFKB p65 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: NFKB p65
Immunogen Range: 51-100/551
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5970
Swiss Prot: Q04206
Source: KLH conjugated synthetic peptide derived from human NFKBp65
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 61
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Chicken
Rabbit
Others
Zebrafish
Predicted Cross Reactive Species:
Dog
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jiang, Wang-Lin, et al. "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." European journal of pharmacology 640.1 (2010): 75-81.Read more>>
- Song, Yong, et al. "NF kappaB expression increases and CFTR and MUC1 expression decreases in the endometrium of infertile patients with hydrosalpinx: a comparative study." Reproductive Biology and Endocrinology 10.1 (2012): 86.Read more>>
- Wang, Chunqiang, Wei Ma, and Yuhong Su. "NF-κB Pathway Contributes to Cadmium-Induced Apoptosis of Porcine Granulosa Cells." Biological trace element research (2013): 1-8.Read more>>
- Zhang, Jingyao, et al. "Effect of hydrogen-rich water on acute peritonitis of rat models." International Immunopharmacology (2014).Read more>>
- Niu, Xiaofeng, et al. "Protective Effects of Chelerythrine Against Lipopolysaccharide-Induced Endotoxic Shock in Mice." Inflammation (2014): 1-8.Read more>>
- Lei, Yongfang, et al. "Potential of grape seed-derived polyphenols extract for protection against testosterone-induced benign prostatic hyperplasia in castrated rats." RSC Advances (2014).Read more>>
- Huang, Di, et al. "Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro." Evidence Based Complementary and Alternative Medicine 2014 (2014).Read more>>
- Luo, Cheng, et al. "Kaempferol alleviates insulin resistance via hepatic IKK/NF-κB signal in type 2 diabetic rats." International Immunopharmacology 28.1 (2015): 744-750.Read more>>
- Ganguly, Rituparna, et al. "Anti-atherogenic Effect of Trivalent Chromium-loaded CPMV Nanoparticles in Human Aortic Smooth Muscle Cells under Hyperglycemic Conditions in vitro." Nanoscale (2016).Read more>>
- Yang, Zixuan, et al. "Hypothermic machine perfusion increases A20 expression which protects renal cells against ischemia/reperfusion injury by suppressing inflammation, apoptosis and necroptosis." International Journal of Molecular Medicine (2016).Read more>>
- Lin, Qin-Qin, et al. "SIRT1 regulates TNF-α-induced expression of CD40 in 3T3-L1 adipocytes via NF-κB pathway." Cytokine 60.2 (2012): 447-455.Read more>>
- Zhao, Zhe, et al. "Inhibition of NF-kappaB activation by Pyrrolidine dithiocarbamate partially attenuates hippocampal MMP-9 activation and improves cognitive deficits in streptozotocin-induced diabetic rats."Behavioural brain research 238 (2013): 44-47.Read more>>
- Yang, Zixuan, et al. "Hypothermic machine perfusion increases A20 expression which protects renal cells against ischemia/reperfusion injury by suppressing inflammation, apoptosis and necroptosis." International journal of molecular medicine (2016).Read more>>
- Zhang, Yuqin, et al. "Ameliorative effects of Gualou Guizhi decoction on inflammation in focal cerebral ischemic-reperfusion injury." Molecular medicine reports 12.1 (2015): 988-994.Read more>>
- Jing, Xue, et al. "Beta2-GPI: a novel factor in the development of hepatocellular carcinoma." Journal of Cancer Research and Clinical Oncology 136.11 (2010): 1671-1680.Read more>>
- Oyagbemi, Ademola Adetokunbo, et al. "Sodium fluoride induces hypertension and cardiac complications through generation of reactive oxygen species and activation of nuclear factor kappa beta." Environmental Toxicology (2016).Read more>>
- Xi, Liqin, et al. "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." Acta Histochemica (2016).Read more>>
- Fattori, Victor, et al. "Vinpocetine reduces diclofenac-induced acute kidney injury through inhibition of oxidative stress, apoptosis, cytokine production, and NF-κB activation in mice." Pharmacological Research (2017).Read more>>
- Zheng, Jian, et al. "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." Journal of Neurosurgery (2016): 1-9.Read more>>
- Zhang et al. High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats. (2015) Int.J.Clin.Exp.Patho. 8:5189-95Read more>>
- Guo et al. Nickel chloride (NiCl2)-caused inflammatory responses via activation of NF-κB pathway and reduction of anti-inflammatory mediator expression in the kidney. (2015) Oncotarge. 6:28607-20Read more>>
- Zhang et al. MicroRNA-26b Modulates the NF-κB Pathway in Alveolar Macrophages by Regulating PTEN. (2015) J.Immuno. 195:5404-14Read more>>
- Lin et al. MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells. (2017) Cell.Physiol.Bioche. 41:124-136Read more>>
- Sun et al. Modulation of stromal cell-derived factor 1 alpha (SDF-1α) and its receptor CXCR4 in Porphyromonas gingivalis-induced periodontal inflammation. (2016) BMC.Oral.Healt. 17:26Read more>>
- Chen et al. Hydrogen sulfide reduces kidney injury due to urinary-derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels. (2014) Exp.Ther.Me. 8:464-470Read more>>
- Li et al. ADAMTS-7 exhibits elevated expression in cartilage of osteonecrosis of femoral head and has a positive correlation with TNF- α and NF- κ B P65. (2015) Mediators.Inflam. 2015:196702Read more>>
- Qian et al. Effect of Daisaikoto on Expressions of SIRT1 and NF-kappaB of Diabetic Fatty Liver Rats Induced by High-Fat Diet and Streptozotocin. (2016) Yonago.Acta.Med. 59:149-58Read more>>
- Xi et al. Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii). (2017) Eur.J.Histochem. 61:2834Read more>>
- Hong et al. Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats. (2018) Mediators.Inflamm. 2017:9783893Read more>>
- Wang et al. Octreotide Protects the Mouse Retina against Ischemic Reperfusion Injury through Regulation of Antioxidation and Activation of NF-κB. (2015) Oxid.Med.Cell.Longev. 2015:970156Read more>>
- Oyagbemi AA et al. Ameliorative effect of Rutin on sodium fluoride‐induced hypertension through modulation of Kim‐1/NF‐κB/Nrf2 signaling pathway in rats.(2018)Environ Toxicol.Read more>>
- Xu J et al.Effect of recombinant Trichinella spiralis cysteine proteinase inhibitor on TNBS-induced experimental inflammatory bowel disease in mice. (2019) Int. Immunopharmacol; Jan;66:28-40.Read more>>
- Chen L et al. Forsythiaside prevents β-amyloid-induced hippocampal slice injury by upregulating 2-arachidonoylglycerol via cannabinoid receptor 1-dependent NF-κB pathway. Neurochem Int. 2019 May;125:57-66.Read more>>
- Han L et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.Nutrients. 2019 Mar 20;11(3).Read more>>
- Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.Read more>>
- Liu W et al. Suppressive effect of glycyrrhizic acid against lipopolysaccharide-induced neuroinflammation and cognitive impairment in C57 mice via toll-like receptor 4 signaling pathway. Food Nutr Res. 2019 Apr 29;63.Read more>>
- Peng LY et al. Baicalin alleviated APEC-induced acute lung injury in chicken by inhibiting NF-κB pathwayactivation. Int Immunopharmacol. 2019 Jul;72:467-472.Read more>>
- Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
- Sallam MY et al. Brainstem cholinergic pathways diminish cardiovascular and neuroinflammatory actions of endotoxemia in rats: Role of NFκB/α7/α4β2AChRs signaling. Neuropharmacology. 2019 Jun 25;157:107683. Read more>>
- Zhang H et al. Dexmedetomidine ameliorates LPS induced acute lung injury via GSK-3β/STAT3-NF-κB signaling pathway in rats. Int Immunopharmacol. 2019 Jun 26;74:105717. Read more>>
- Han X et al. SUMOylation and deacetylation affect NF-κB p65 activity induced by high glucose in human lens epithelial cells. Int J Ophthalmol. 2019 Sep 18;12(9):1371-1379. Read more>>
- Wang Y et al. H2S mediates apoptosis in response to inflammation through PI3K/Akt/NFκB signaling pathway. Biotechnol Lett. 2019 Dec 23. Read more>>
- Wu ZY et al. Baicalin Attenuates Mycoplasma gallisepticum-Induced Inflammation via Inhibition of the TLR2-NF-κB Pathway in Chicken and DF-1 Cells. Infection and Drug Resistance 2019:12 3911–3923.Read more>>
- Wang Y et al. Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats. Neurobiol Learn Mem. 2019 Dec 16;168:107141.Read more>>
- Wedn AM et al. Time and sex dependency of hemodynamic, renal, and survivability effects of endotoxemia in rats. Saudi Pharm J. 2020 Jan;28(1):127-135.Read more>>
- Duan S et al. Effect of vitexin on alleviating liver inflammation in a dextran sulfate sodium (DSS)-induced colitis model. Biomed Pharmacother. 2020 Jan;121:109683. Read more>>
- Liu B et al. The atherosclerosis-ameliorating effects and molecular mechanisms of BuYangHuanWu decoction. Biomed Pharmacother. 2019 Dec 27;123:109664. Read more>>
- Zhao M et al. Non-proteolytic ubiquitination of OTULIN regulates NF-κB signaling pathway. J Mol Cell Biol. 2019 Sep 3. pii: mjz081. Read more>>
- Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
- Gao R et al. Quasi-ultrafine particles promote cell metastasis via HMGB1-mediated cancer cell adhesion. Environ Pollut. 2019 Oct 23:113390. Read more>>
VALIDATION IMAGES
HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated (bs-0465R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (bs-0465R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 overnight at 4°C, DAB staining.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NFKB p65 Antibody(bs-0465R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (NFKB p65) polyclonal Antibody, Unconjugated (bs-0465R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (NFKB p65) polyclonal Antibody, Unconjugated (bs-0465R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (NFKB p65) polyclonal Antibody, Unconjugated (bs-0465R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.