Host: Rabbit
Target Protein: Thioredoxin
Immunogen Range: 51-105/105
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7295
Swiss Prot: P10599
Source: KLH conjugated synthetic peptide derived from human Thioredoxin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 12
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Li, Mo-fei, et al. "First characterization of a teleost Epstein-Barr virus-induced gene 3 (EBI3) reveals a regulatory effect of EBI3 on the innate immune response of peripheral blood leukocytes." Developmental & Comparative Immunology 41.4 (2013): 514-522.Read more>>
- Gao, Fuping, et al. "Cytotoxicity and therapeutic effect of irinotecan combined with selenium nanoparticles." Biomaterials (2014).Read more>>
- Zhang, Junjie, et al. "Screening and identification of post-traumatic stress-related serum factors in children with Wilms tumors." Oncology Letters 11.2 (2016): 1299-1304.Read more>>
- Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-B signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965Read more>>
- Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
Paraformaldehyde-fixed, paraffin embedded human cervical carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Thioredoxin Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33018P) probed with Thioredoxin/Trx Polyclonal Antibody, Unconjugated (bs-0458R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Thioredoxin) polyclonal Antibody, Unconjugated (bs-0458R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Recombinant CKC-835 protein, Trx & S & His(bs-49104P) probed with Thioredoxin Polyclonal Antibody, Unconjugated (bs-0458R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.