bs-0444R [Primary Antibody]
Slc22A17 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Slc22A17

Immunogen Range: 475-538/538


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51310

Swiss Prot: Q8WUG5

Source: KLH conjugated synthetic peptide derived from human SLC22A17

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wu, Limin, et al. "Lipocalin‐2 enhances angiogenesis in rat brain endothelial cells via reactive oxygen species and iron‐dependent mechanisms." Journal of Neurochemistry (2015).Read more>>
  • Cui et al. NGALR is overexpressed and regulated by hypomethylation in esophageal squamous cell carcinoma. (2008) Clin.Cancer.Res. 14:7674-81Read more>>
  • Lv et al. Overexpression of neutrophil gelatinase-associated lipocalin and its receptor in colorectal carcinoma: Significant correlation with cell differentiation and tumour invasion. (2010) Oncol.Lett. 1:103-108Read more>>
  • Du Y et al. Effects of lipocalin-2 on brain endothelial adhesion and permeability. PLoS One. 2019 Jul 3;14(7):e0218965. Read more>>
  • Sarah K. Schr?der. et al. Lipocalin 2 receptors: facts, fictions, and myths. FRONT IMMUNOL. 2023; 14: 1229885Read more>>
  • Sarah K. Schr?der. et al. Lipocalin 2 receptors: facts, fictions, and myths. FRONT IMMUNOL. 2023; 14: 1229885Read more>>
  • Wenlu Li. et al. SLC22A17 as a Cell DeathLinked Regulator of Tight Junctions in Cerebral Ischemia. STROKE. 2024Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat colon carcinoma labeled with Anti-Slc22A17 Y Polyclonal Antibody, Unconjugated (bs-0444R) at 1:300, followed by conjugation to the secondary antibody and DAB staining


Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human U-2OS cell lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human Siha cell lysates probed with Slc22A17 Polyclonal Antibody, Unconjugated (bs-0444R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.