bs-0423R
[Primary Antibody]
MMP-7 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MMP-7
Immunogen Range: 151-250/269
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from mouse MMP7
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 30
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhu, Hongwu, et al. "Activating Transcription Factor 4 Promotes Esophageal Squamous Cell Carcinoma Invasion and Metastasis in Mice and Is Associated with Poor Prognosis in Human Patients." PloS one 9.7 (2014): e103882.Read more>>
- Madka, Venkateshwar, et al. "TP53 modulating agent, CP-31398 enhances antitumor effects of ODC inhibitor in mouse model of urinary bladder transitional cell carcinoma." American Journal of Cancer Research 5.10 (2015): 3030.Read more>>
- Jeinsen, Niklas, et al. "Biocompatibility of intensified decellularized equine carotid arteries in a rat subcutaneous implantation model and in a human in vitro-model." Tissue Engineering (2017).Read more>>
- Yang Lv. et al. TGF-1 enhanced myocardial differentiation through inhibition of the Wnt/-catenin pathway with rat BMSCs. Iran J Basic Med Sci. 2020 Aug; 23(8): 1012C1019Read more>>
- Wang Chengqin. et al. Silencing of KIF3B Suppresses Breast Cancer Progression by Regulating EMT and Wnt/-Catenin Signaling. Front Oncol. 2021 Jan;10:3063Read more>>
- Wenwen Chen. et al. Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification. Drug Des Dev Ther. 2021; 15: 4125C414Read more>>
- Wu, Haibin. et al. Dextran sulfate prevents excess aggregation of human pluripotent stem cells in 3D culture by inhibiting ICAM1 expression coupled with down-regulating E-cadherin through activating the Wnt signaling pathway. STEM CELL RES THER. 2022 Dec;13(1):1-2Read more>>
- Wu, Bo. et al. Diagnostic values of plasma matrix metalloproteinase-7, interleukin-8, and gamma-glutamyl transferase in biliary atresia. EUR J PEDIATR. 2022 Sep;:1-9Read more>>
- Li, Jun. et al. Immunoproteasome inhibition prevents progression of castration-resistant prostate cancer. BRIT J CANCER. 2023 Jan;:1-14Read more>>
- Amei Tang. et al. Tuberostemonine may alleviates proliferation of lung fibroblasts caused by pulmonary fibrosis. INT J IMMUNOPATH PH. ;()Read more>>
VALIDATION IMAGES
Mouse Uterus lysates probed with MMP-7 Polyclonal Antibody, unconjugated (bs-0423R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-MMP-7 Polyclonal Antibody, Unconjugated(bs-0423R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-MMP-7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrium; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.