bs-0415R
[Primary Antibody]
TIMP-1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: TIMP-1
Immunogen Range: 103-207/207
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7076
Swiss Prot: P01033
Source: KLH conjugated synthetic peptide derived from human TIMP-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP1, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 21
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Sassoli, Chiara, et al. "Defining the role of mesenchymal stromal cells on the regulation of matrix metalloproteinases in skeletal muscle cells." Experimental Cell Research (2014).Read more>>
- Lu HY, Huang CY, Shih CM, Chang WH, Tsai CS, et al. (2015) Dipeptidyl Peptidase-4 Inhibitor Decreases Abdominal Aortic Aneurysm Formation through GLP-1-Dependent Monocytic Activity in Mice. PLoS ONE 10(4): e0121077.Read more>>
- Ni, Wei-Jian, et al. "Renoprotective effects of berberine through regulation of the MMPs/TIMPs system in streptozocin-induced diabetic nephropathy in rats."European Journal of Pharmacology (2015).Read more>>
- Watts, Ryan P., et al. "Novel 24-h ovine model of brain death to study the profile of the endothelin axis during cardiopulmonary injury." Intensive Care Medicine Experimental 3.1 (2015): 31.Read more>>
- Sassoli, Chiara, et al. "Low intensity 635 nm diode laser irradiation inhibits fibroblast–myofibroblast transition reducing TRPC1 channel expression/activity: New perspectives for tissue fibrosis treatment." Lasers in Surgery and Medicine (2016).Read more>>
- Lu, Minling, et al. "The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage." Cells Tissues Organs 196.3 (2012): 241-250.Read more>>
- Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).Read more>>
- Jiang, Li, Tongfu Zhou, and Hanming Liu. "Combined effects of the ATP-sensitive potassium channel opener pinacidil and simvastatin on pulmonary vascular remodeling in rats with monocrotaline-induced pulmonary arterial hypertension." Die Pharmazie-An International Journal of Pharmaceutical Sciences 67.6 (2012): 547-552.Read more>>
- Liang et al. Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating autophagy. (2017) Int.J.Mol.Me. 40:1781-1791Read more>>
- Liu et al. Hydrogen coadministration slows the development of COPD-like lung disease in a cigarette smoke-induced rat model. (2017) Int.J.Chron.Obstruct.Pulmon.Di. 12:1309-1324Read more>>
- Li et al. Neurogenic differentiation factor NeuroD confers protection against radiation-induced intestinal injury in mice. (2016) Sci.Rep. 6:30180Read more>>
- Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014).Read more>>
- Zhao R et al. Interleukin-1 receptor antagonist protein (IL-1Ra) and miR-140 overexpression via pNNS-conjugated chitosan-mediated gene transfer enhances the repair of full-thickness cartilage defects in a rabbit model. Bone Joint Res. 2019 Apr 2;8(3):165-178.Read more>>
- Varghese S et al. Immunostimulatory plant polysaccharides impede cancer progression and metastasis by avoiding off-target effects. Int Immunopharmacol. 2019 May 21;73:280-292.Read more>>
- Tort S et al. The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Burns. 2019 Dec 17. pii: S0305-4179(18)30976-8.Read more>>
- Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
- Xu T et al. MicroRNA\708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.Read more>>
- Mengmeng Zhang. et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachlorideCinduced liver fibrosis in mice. N-S Arch Pharmacol. 2021 Jan;394(1):189-204Read more>>
- Xiaolan Ouyang. et al. Transplantation of Human Amnion Epithelial Cells Improves Endometrial Regeneration in Rat Model of Intrauterine Adhesions. Stem Cells Dev. 2020 Oct;29(20):1346-1362Read more>>
- Xia Liu. et al. The adipokine orosomucoid alleviates adipose tissue fibrosis via the AMPK pathway. Acta Pharmacol Sin. 2021 Apr;:1-9Read more>>
- Shi, Bohui. et al. Polymorphism of MMP-3 gene and imbalance expression of MMP-3 / TIMP-1 in articular cartilage are associated with an endemic osteochondropathy, Kashin- Beck disease. Bmc Musculoskel Dis. 2022 Dec;23(1):1-7Read more>>
- Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022Read more>>
- Choirul Anwar. et al. Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy. J FUNCT FOODS. 2022 Sep;96:105226Read more>>
- Chi Li. et al. Schisantherin D from Schisandra chinensis (Turcz.) Baill. exhibits anti-liver fibrosis capacity via modulating ETBR involved signaling, an in vitro and in vivo study. FITOTERAPIA. 2022 Oct;162:10529Read more>>
- Guo, Ziwei. et al. Single and Combined Effects of Short-Term Selenium Deficiency and T-2 Toxin-Induced Kidney Pathological Injury Through the MMPs/TIMPs System. BIOL TRACE ELEM RES. 2023 Jan;:1-11Read more>>
- Gundogdu, Gokhan. et al. Characterization of a novel rabbit model of Peyronies disease. INT J IMPOT RES. 2023 Feb;:1-6Read more>>
- linlin zeng. et al. Atractylodes Processing Products Protect against Gastric Ulcers in Rats by Influencing the NF-B-MMP-9/TIMP-1 Regulatory Mechanism and Intestinal Flora. CHEM BIODIVERS. 2023 Apr;:e202300068Read more>>
- Yang Liu. et al. Paeoniflorin Coordinates Macrophage Polarization and Mitigates Liver Inflammation and Fibrogenesis by Targeting the NF-B/HIF-1 Pathway in CCl4-Induced Liver Fibrosis. AM J CHINESE MED. 2023 Jun 14Read more>>
- Shimoji Kiyofumi. et al. Hypoxia-inducible factor 1 modulates interstitial pneumonia-mediated lung cancer progression. J TRANSL MED. 2023 Dec;21(1):1-19Read more>>
- Lei Han. et al. Platelet-rich plasma-derived exosomes promote rotator cuff tendon-bone healing. INJURY. 2024 Feb;55:111212Read more>>
- Demircan Volkan. et al. Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis. SCI REP-UK. 2024 Mar;14(1):1-14Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-TIMP-1(NT) Polyclonal Antibody, Unconjugated(bs-0415R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit liver tissue labeled with Rabbit Anti-TIMP-1(NT) Polyclonal Antibody, Unconjugated (bs-0415R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-TIMP-1(NT) Polyclonal Antibody, Unconjugated(bs-0415R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Brain; Lane 2: Mouse Liver; 30ug loaded into the lane; Probed with TIMP-1 Polyclonal Antibody, Unconjugated (bs-0415R) at 1:200 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Tissue/cell: Human Gastric Carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0 ), Boiling for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (bs-0415R) 1:200, overnight at 4°C, followed by incubation with secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human Neurilemmoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ChRM2 Polyclonal Antibody, Unconjugated (bs-0236R) at 1:500 overnight at 4°C, DAB staining.