bs-0412R-PE [Conjugated Primary Antibody]
MMP2 Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MMP2

Immunogen Range: 31-109/476


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4313

Swiss Prot: P08253

Source: MAT conjugated synthetic peptide derived from human MMP2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 72


Cross Reactive Species: Human
Mouse
Rat
Rabbit
Others

Predicted Cross Reactive Species: Cow
Sheep
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wu, Kaijie, et al. "PI3K/Akt to GSK3β/β-catenin signaling cascade coordinates cell colonization for bladder cancer bone metastasis through regulating ZEB1 transcription." Cellular signalling (2012).Read more>>
  • Wu, Kaijie, et al. "Silibinin inhibits β-catenin/ZEB1 signaling and suppresses bladder cancer metastasis via dual-blocking epithelial-mesenchymal transition and stemness." Cellular Signalling (2013).Read more>>
  • Qin, YuanHua, et al. "Recombinant human CXCL8 (3‐72) K11R/G31P regulates smooth muscle cell proliferation and migration through blockage of interleukin‐8 receptor." IUBMB life 65.1 (2013): 67-75.Read more>>
  • Fang, Ming, Xin-Chi Wu, and Wenlong Huang. "Raloxifene Upregulated Mesangial Cell MMP-2 Activity via ER-β Through Transcriptional Regulation." Cell Biochemistry and Biophysics (2013): 1-7.Read more>>
  • Zhu, Hongwu, et al. "Activating Transcription Factor 4 Promotes Esophageal Squamous Cell Carcinoma Invasion and Metastasis in Mice and Is Associated with Poor Prognosis in Human Patients." PLoS one 9.7 (2014): e103882.Read more>>
  • Ni, Wei-Jian, et al. "Renoprotective effects of berberine through regulation of the MMPs/TIMPs system in streptozocin-induced diabetic nephropathy in rats."European Journal of Pharmacology (2015).Read more>>
  • Madka, Venkateshwar, et al. "TP53 modulating agent, CP-31398 enhances antitumor effects of ODC inhibitor in mouse model of urinary bladder transitional cell carcinoma." American Journal of Cancer Research 5.10 (2015): 3030.Read more>>
  • Wu, Kaijie, et al. "Silibinin reverses epithelial-to-mesenchymal transition in metastatic prostate cancer cells by targeting transcription factors." Oncology Reports 23.6 (2010): 1545.Read more>>
  • Hiramoto, K., Y. Yamate, and E. F. Sato. "Long-Term Ultraviolet A Eye Irradiation Causes Retina Denaturation in Mice." Biomedicine Hub 2.1 (2017): 5-5.Read more>>
  • Wang, Li, et al. "Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model." Peptides (2017).Read more>>
  • Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).Read more>>
  • Vendrov, Aleksandr E., et al. "Attenuated Superoxide Dismutase 2 Activity Induces Atherosclerotic Plaque Instability During Aging in Hyperlipidemic Mice." Journal of the American Heart Association 6.11 (2017): e006775.Read more>>
  • Gao, Yuan, et al. "Effect of Telmisartan on Preventing Learning and Memory Deficits Via Peroxisome Proliferator-Activated Receptor-γ in Vascular Dementia Spontaneously Hypertensive Rats." Journal of Stroke and Cerebrovascular Diseases (2017).Read more>>
  • Kumar et al. Simultaneous targeting of 5-LOX-COX and ODC block NNK-induced lung adenoma progression to adenocarcinoma in A/J mice. (2016) Am.J.Cancer.Re. 6:894-909Read more>>
  • Ren et al. Interferon-γ and celecoxib inhibit lung-tumor growth through modulating M2/M1 macrophage ratio in the tumor microenvironment. (2014) Drug.Des.Devel.Ther. 8:1527-38Read more>>
  • Li et al. Visfatin Destabilizes Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice. (2016) PLoS.One. 11:e0148273Read more>>
  • Yan et al. Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway. (2016) PLoS.One. 11:e0148536Read more>>
  • Du et al. MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a. (2015) Sci.Rep. 5:16163Read more>>
  • Moriwaki et al. TRKB tyrosine kinase receptor is a potential therapeutic target for poorly differentiated oral squamous cell carcinoma. (2018) Oncotarget. 9:25225-25243Read more>>
  • Zhang J et al. Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation. (2019)Biomedicine & Pharmacotherapy, 109, 806–814. Read more>>
  • Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55.Read more>>
  • Lei Z et al. Array-Based in situ Fluorescence Assay for Profiling Multiplex Matrix Metalloproteinases Activities in Tissue Section. Analytica Chimica Acta.2019 Read more>>
  • Chang J et al. miR‐363‐3p inhibits migration, invasion, and epithelial–mesenchymal transition by targeting NEDD9 and SOX4 in non‐small‐cell lung cancer. J Cell Physiol. 2019 Jul 22. Read more>>
  • Chang J et al. miR‐363‐3p inhibits migration, invasion, and epithelial–mesenchymal transition by targeting NEDD9 and SOX4 in non‐small‐cell lung cancer. J Cell Physiol. 2019 Jul 22. Read more>>
  • Han S et al. The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway. Biochem Cell Biol. 2019 Jan 24. Read more>>
  • Saribas GS et al. Effects of uterus derived mesenchymal stem cells and their exosomes on asherman's syndrome. Acta Histochem. 2019 Nov 24:151465.Read more>>
  • Luo Z et al. Ligustilide Inhibited Rat Vascular Smooth Muscle Cells Migration via c‐Myc/MMP2 and ROCK/JNK Signaling Pathway. J Food Sci. 2019 Nov 24. Read more>>
  • Tort S et al. The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Burns. 2019 Dec 17. pii: S0305-4179(18)30976-8.Read more>>
  • Han N et al. MiR-203a-3p inhibits retinal angiogenesis and alleviates proliferative diabetic retinopathy in oxygen-induced retinopathy (OIR) rat model via targeting VEGFA and HIF-1α. Clin Exp Pharmacol Physiol. 2019 Aug 13. Read more>>
  • Wang W et al. Ginkgo biloba extract may alleviate viral myocarditis by suppression of S100A4 and MMP‐3. J Med Virol. 2019 Jul 30. Read more>>
  • Zhou L et al. The role of miR-21/RECK in the inhibition of osteosarcoma by curcumin. Mol Cell Probes. 2020 Feb 17:101534. Read more>>
  • Du J et al. MAY, a novel tubulin inhibitor, induces cell apoptosis in A549 and A549/Taxol cells and inhibits epithelial-mesenchymal transition in A549/Taxol cells. Chem Biol Interact. 2020 May 25;323:109074.Read more>>
  • Xu T et al. MicroRNA\708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.Read more>>
  • Gong H et al. EZH2 inhibitors reverse resistance to gefitinib in primary EGFR wild-type lung cancer cellsBMC Cancer.2020 Dec 4;20(1):1189.Read more>>
  • Yanhong Wang. et al. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats. Drug Des Dev Ther. 2020; 14: 4825C4834Read more>>
  • Hongzhong Cheng. et al. Circular RNA circLPAR3 Facilitates Esophageal Squamous Cell Carcinoma Progression Through Upregulating HMGB1 via Sponging miR-375/miR-433. Oncotargets Ther. 2020; 13: 7759C7771Read more>>
  • Chen Chenglong. et al. miR-135a Reduces Osteosarcoma Pulmonary Metastasis by Targeting Both BMI1 and KLF4. Front Oncol. 2021 Mar;11:93Read more>>
  • Ozge Petek Erpolat. et al. Angiotensin-converting Enzyme Inhibitor Reduces Radiation-induced Periprosthetic Capsular Fibrosis. J Surg Res. 2021 Jul;263:167Read more>>
  • Xia Liu. et al. The adipokine orosomucoid alleviates adipose tissue fibrosis via the AMPK pathway. Acta Pharmacol Sin. 2021 Apr;:1-9Read more>>
  • Liting Cheng. et al. Bioresponsive micro-to-nano albumin-based systems for targeted drug delivery against complex fungal infections. Acta Pharm Sin B. 2021 MayRead more>>
  • Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634Read more>>
  • Wang, Huihui. et al. Anti-inflammatory and Antioxidant Effects of Pyrroloquinoline Quinone in L-NAME-Induced Preeclampsia-Like Rat Model. 2021 Sep 2Read more>>
  • Sudeshna Nandi. et al. Anti-cancer effect of astrakurkurol from a folklore tribal mushroom on human hepatocellular carcinoma cells via mediating cell cycle inhibition, apoptosis, and migration. 2021 Nov 22Read more>>
  • Wen Xiong. et al. Mechanisms and Molecular Targets of BuShenHuoXue Formula for Osteoarthritis. Acs Omega. 2022;7(5):47034713Read more>>
  • Duan, Houyu. et al. KISS-1, Mediated by Promoter Methylation, Suppresses Esophageal Squamous Cell Carcinoma Metastasis via MMP2/9/MAPK Axis. Digest Dis Sci. 2022 Jan;:1-17Read more>>
  • Shuyuan Wu. et al. miR-34a-5p inhibits the malignant progression of KSHV-infected SH-SY5Y cells by targeting c-fos. PEERJ. 2022 Apr;10:e13233Read more>>
  • Xu Huang. et al. Osteopontin-Targeted and PPAR-Agonist-Loaded Nanoparticles Efficiently Reduce Atherosclerosis in Apolipoprotein E/ Mice. ACS OMEGA. 2022;7(33):2876728778Read more>>
  • Shugen Xu. et al. Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringers solution. ANN TRANSL MED. 2022 Dec; 10(24): 1331Read more>>
  • Qianfeng Liu. et al. Perfluoroalkyl substances promote breast cancer progression via ER and GPER mediated PI3K/Akt and MAPK/Erk signaling pathways. ECOTOX ENVIRON SAFE. 2023 Jun;258:11498Read more>>
  • Yong Wang. et al. An integrated network pharmacology approach reveals that Darutigenol reduces inflammation and cartilage degradation in a mouse collagen-induced arthritis model by inhibiting the JAK-STAT3 pathway. J ETHNOPHARMACOL. 2023 May;:116574Read more>>
VALIDATION IMAGES