Host: Rabbit
Target Protein: Leptin
Immunogen Range: 17-100/167
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 280836
Swiss Prot: P50595
Source: KLH conjugated synthetic peptide derived from bovine Leptin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 16
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species:
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017).Read more>>
- Zhang, Yun, et al. "Correlation analysis between the expressions of leptin and its receptor (ObR) and clinicopathology in endometrial cancer." Cancer Biomarkers 14.5 (2014): 353-359.Read more>>
- Li F et al. The Nutritional Cytokine Leptin Promotes NSCLC by Activating the PI3K/AKT and MAPK/ERK Pathways in NSCLC Cells in a Paracrine Manner. Biomed Res Int. 2019 Apr 18;2019:2585743.Read more>>
- Jisheng Wang. et al. Biological Network Model of Effect of Chronic Intermittent Hypoxia on Spermatogenesis in Rats. Med Sci Monitor. 2020; 26: e925579-1Ce925579-11Read more>>
- Jun Deng. et al. The metabolic hormone leptin promotes the function of T FH cells and supports vaccine responses. Nat Commun. 2021 May;12(1):1-15Read more>>
- Kimberly A. Clark. et al. Evaluation of the Central Effects of Systemic Lentiviral-Mediated Leptin Delivery in Streptozotocin-Induced Diabetic Rats. Int J Mol Sci. 2021 Jan;22(24):13197Read more>>
- Michelotti Tainara C.. et al. Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows. FRONT CELL DEV BIOL. 2022 Oct;0:2076Read more>>
- Pan Jianping. et al. Hypolipidemic effect of ethanol extract from Chimonanthus nitens Oliv. leaves in hyperlipidemia rats via activation of the leptin/JAK2/STAT3 pathway. MOL MED. 2022 Dec;28(1):1-12Read more>>
- Fangda Fu. et al. AR/PCC herb pair inhibits osteoblast pyroptosis to alleviate diabetes-related osteoporosis by activating Nrf2/Keap1 pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 AugRead more>>
- Wang Jianan. et al. Association of attenuated leptin signaling pathways with impaired cardiac function under prolonged high-altitude hypoxia. SCI REP-UK. 2024 May;14(1):1-18Read more>>
Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti-Leptin Polyclonal Antibody (bs-0409R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:400 overnight at 4°C, DAB staining.
Leptin lysates probed with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Leptin Polyclonal Antibody, Unconjugated (bs-0409R) at 1:200 overnight at 4°C, DAB staining.