bs-0397R [Primary Antibody]
MMP9 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MMP9

Immunogen Range: 611-707/707


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4318

Swiss Prot: P14780

Source: KLH conjugated synthetic peptide derived from human MMP9

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 78


Cross Reactive Species: Human
Mouse
Rat
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qin, YuanHua, et al. "Recombinant human CXCL8 (3‐72) K11R/G31P regulates smooth muscle cell proliferation and migration through blockage of interleukin‐8 receptor." IUBMB life 65.1 (2013): 67-75.Read more>>
  • Basak, Sanjay, et al. "The interplay between glucose and fatty acids on tube formation and fatty acid uptake in the first trimester trophoblast cells, HTR8/SVneo." Molecular and Cellular Biochemistry (2014): 1-9.Read more>>
  • Koronyo, Yosef, et al. "Therapeutic effects of glatiramer acetate and grafted CD115+ monocytes in a mouse model of Alzheimers disease." Brain (2015): awv150.Read more>>
  • Sulistyowati, Sri, Yuniarsih Zakia, and Soetrisno Khasan. "High MMP-9 and TNF-α expression increase in preterm premature rupture of membranes."Universa Medicina 35.1 (2016): 33-39.Read more>>
  • Zhao, Zhe, et al. "Inhibition of NF-kappaB activation by Pyrrolidine dithiocarbamate partially attenuates hippocampal MMP-9 activation and improves cognitive deficits in streptozotocin-induced diabetic rats."Behavioural brain research 238 (2013): 44-47.Read more>>
  • Borin, Thaiz F., et al. "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." PLoS One 12.6 (2017).Read more>>
  • Wu et al. Subchondral bone changes and chondrogenic capacity of progenitor cells from subchondral bone in the collagenase-induced temporomandibular joints osteoarthritis rabbit model. (2015) Int.J.Clin.Exp.Patho. 8:9782-9Read more>>
  • Li et al. Visfatin Destabilizes Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice. (2016) PLoS.One. 11:e0148273Read more>>
  • Yan et al. Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway. (2016) PLoS.One. 11:e0148536Read more>>
  • Hapsari,et al.Hylocereus Polyrhizus Peel Ethanol Extract- the Potential Effect to Tumor Necrosis Factor-Á, Macrophage, and Matrix Metalloproteinase-9 in Endometriosis Mice.(2017) Journal of International Dental and Medical Research. 10:1073-1079.Read more>>
  • Takahashi,et al.Novel phytopeptide osmotin mimics preventive effects of adiponectin on vascular inflammation and atherosclerosis.(2018) Metabolism: Clinical and Experimental. 83:128-138.Read more>>
  • Zhao Q et al. Anticancer effects of plant derived Anacardic acid on human breast cancer MDA-MB-231 cells.(2018) Am J Transl Res.Aug 15;10(8):2424-2434.Read more>>
  • Zhang J et al. Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation. (2019)Biomedicine & Pharmacotherapy, 109, 806–814. Read more>>
  • Chang J et al. miR‐363‐3p inhibits migration, invasion, and epithelial–mesenchymal transition by targeting NEDD9 and SOX4 in non‐small‐cell lung cancer. J Cell Physiol. 2019 Jul 22. Read more>>
  • Han S et al. The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway. Biochem Cell Biol. 2019 Jan 24. Read more>>
  • Saribas GS et al. Effects of uterus derived mesenchymal stem cells and their exosomes on asherman's syndrome. Acta Histochem. 2019 Nov 24:151465.Read more>>
  • Tort S et al. The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Burns. 2019 Dec 17. pii: S0305-4179(18)30976-8.Read more>>
  • Han N et al. MiR-203a-3p inhibits retinal angiogenesis and alleviates proliferative diabetic retinopathy in oxygen-induced retinopathy (OIR) rat model via targeting VEGFA and HIF-1α. Clin Exp Pharmacol Physiol. 2019 Aug 13. Read more>>
  • Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.Read more>>
  • Ayu I et al. The Effect of (E)-1-(4'-aminophenyl)-3-phenylprop-2-en-1-one on MicroRNA-18a, Dicer1, and MMP-9 Expressions against DMBA-Induced Breast Cancer. Asian Pac J Cancer Prev. 2020 May 1;21(5):1213-1219.Read more>>
  • Tong Xu. et al. Lithium chloride represses abdominal aortic aneurysm via regulating GSK3/SIRT1/NF-B signaling pathway. Free Radical Bio Med. 2021 Apr;166:1Read more>>
  • Yanhong Wang. et al. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats. Drug Des Dev Ther. 2020; 14: 4825C4834Read more>>
  • Hirokazu Konishi. et al. Effect of pemafibrate (K-877), a novel selective peroxisome proliferator-activated receptor modular (SPPARM), in atherosclerosis model using low density lipoprotein receptor knock-out swine with balloon injury. Plos One. 2020 Nov;15(11):e0241195Read more>>
  • Ida Ayu Ika Wahyuniari. et al. The Effect of (E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one on MicroRNA-18a, Dicer1, and MMP-9 Expressions against DMBA-Induced Breast Cancer. Asian Pac J Cancer P. 2020 May; 21(5): 1213C1219Read more>>
  • Hongzhong Cheng. et al. Circular RNA circLPAR3 Facilitates Esophageal Squamous Cell Carcinoma Progression Through Upregulating HMGB1 via Sponging miR-375/miR-433. Oncotargets Ther. 2020; 13: 7759C7771Read more>>
  • Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-308Read more>>
  • Jin, Meiyuan. et al. Role of ARID1A in the Regulation of Human Trophoblast Migration and Invasion. 2021 Jul 13Read more>>
  • Wenwen Chen. et al. Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification. Drug Des Dev Ther. 2021; 15: 4125C414Read more>>
  • Guansheng Liao. et al. Long non-coding RNA nuclear enriched abundant transcript 1 (NEAT1) modulates inhibitor of DNA binding 1 (ID1) to facilitate papillary thyroid carcinoma development by sponging microRNA-524-5p. BIOENGINEERED. 2022;13(5):13201-13212Read more>>
  • Jialin Meng. et al. Macrophages and monocytes mediated activation of oxidative phosphorylation implicated the prognosis and clinical therapeutic strategy of Wilms tumour. COMPUT STRUCT BIOTEC. 2022 JuRead more>>
  • Fang Dai. et al. Effects of long term diabetogenic high fat diet on bone in ovariectomized female rats. BIOTECH HISTOCHEM. 2022 Jun 28Read more>>
  • Orbach, Sophia M.. et al. Single-cell RNA-sequencing identifies anti-cancer immune phenotypes in the early lung metastatic niche during breast cancer. CLIN EXP METASTAS. 2022 Aug;:1-17Read more>>
  • Gurur Garip. et al. Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death. ACTA HISTOCHEM. 2022 Oct;124:151951Read more>>
  • Huan Lei. et al. A Combination Therapy Using Electrical Stimulation and Adaptive, Conductive Hydrogels Loaded with Self-Assembled Nanogels Incorporating Short Interfering RNA Promotes the Repair of Diabetic Chronic Wounds. Advanced Science. 2022 Sep;:2201425Read more>>
  • Xinghe Chen. et al. Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif. TOXICOL APPL PHARM. 2022 Dec;457:116319Read more>>
  • Pallavi Shyam Kaparekar. et al. In vitro and in vivo effect of novel GA-CSNPs loaded col-fibrin nanocomposite scaffold on diabetic wound healing. J BIOMED MATER RES B. 2022 DecRead more>>
  • Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:11664Read more>>
  • Liqun Song. et al. Plasma fibrin membranes loaded with bone marrow mesenchymal stem cells and corneal epithelial cells promote corneal injury healing via attenuating inflammation and fibrosis after corneal burns. BIOMATER SCI-UK. 2023 JulRead more>>
  • Xin Cao. et al. Alleviation of temporomandibular joint osteoarthritis by targeting RIPK1-mediated inflammatory signalling. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 AugRead more>>
  • Sun Jia. et al. ANKRD49 promotes the metastasis of NSCLC via activating JNK-ATF2/c-Jun-MMP-2/9 axis. BMC CANCER. 2023 Dec;23(1):1-18Read more>>
  • Zhou Minglu. et al. Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition. NAT COMMUN. 2024 Mar;15(1):1-21Read more>>
  • Demircan Volkan. et al. Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis. SCI REP-UK. 2024 Mar;14(1):1-14Read more>>
  • Yuanyuan Wang. et al. Combined ROS Sensitive Folate Receptor Targeted Micellar Formulations of Curcumin Effective Against Rheumatoid Arthritis in Rat Model. INT J NANOMED. 2024 13Read more>>
  • Gelin Xiang. et al. Revealing the potential bioactive components and mechanism of Qianhua Gout Capsules in the treatment of gouty arthritis through network pharmacology, molecular docking and pharmacodynamic study strategies. HELIYON. 2024 May;1Read more>>
  • Ling Tang. et al. Construction of ROS-Responsive Hyaluronic Acid Modified Paclitaxel and Diosgenin Liposomes and Study on Synergistic Enhancement of Anti-Ovarian Cancer Efficacy. INT J NANOMED. 2024 04Read more>>
VALIDATION IMAGES

Image kindly submitted by Hailing Yang. Human glioblastoma cell line (U87) probed with Rabbit Anti-MMP9 Polyclonal Antibody (bs-0397R).


Formalin-fixed and paraffin embedded human lung caricoma labeled with Anti-MMP-9 Polyclonal Antibody, Unconjugated (bs-0397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat ovary carcinoma labeled with Anti-MMP-9 Polyclonal Antibody, Unconjugated (bs-0397R) at 1:200, followed by conjugation to the secondary antibody and DAB staining