bs-0384R [Primary Antibody]
GLUT4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GLUT4

Immunogen Range: 401-509/509


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6517

Swiss Prot: P14672

Source: KLH conjugated synthetic peptide derived from human GLUT4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse
Rat
Pig
Sheep
Goat

Predicted Cross Reactive Species: Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Shihai, et al. "Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2." British Journal of Nutrition (2016): 1-10.Read more>>
  • Gao, Sujie, et al. "Propofol inhibits growth of neurons through regulating insulin receptor and insulin-like growth factor-1 receptor." Int J Clin Exp Pathol 9.7 (2016): 6785-6794.Read more>>
  • Sikder et al. High Fat Diet Upregulates Fatty Acid Oxidation and Ketogenesis via Intervention of PPAR-γ. (2018) Cell.Physiol.Biochem. 48:1317-1331Read more>>
  • Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193.Read more>>
  • Ye X et al. Irisin reverses insulin resistance in C2C12 cells via the p38-MAPK-PGC-1α pathway. Peptides. 2019 Jul 24:170120. Read more>>
  • Pan LL et al. Urinary Metabolomics Study of the Intervention Effect of Hypoglycemic Decoction on Type 2 Diabetes Mellitus Rats Model. Evidence-Based Complementary and Alternative Medicine, 2019, 1–17. Read more>>
  • Li Q et al. All-trans retinoic acid regulates sheep primary myoblasts proliferation and differentiation in vitro. Domestic Animal Endocrinology,2019 106394. Read more>>
  • Meng-fan Peng. et al. Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet. J Ethnopharmacol. 2021 Jun;273:113947Read more>>
  • Liza D. Morales. et al. Further evidence supporting a potential role for ADH1B in obesity. Sci Rep-Uk. 2021 Jan;11(1):1-14Read more>>
  • Zhang Yan-hui. et al. -Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1 Signaling Pathway in P301S Mice. Front Aging Neurosci. 2020 Aug;12:262Read more>>
  • Pengli Wang. et al. The AT1 receptor autoantibody causes hypoglycemia in fetal rats via promoting the STT3A-GLUT1-glucose uptake axis in liver. Mol Cell Endocrinol. 2020 Dec;518:111022Read more>>
  • Xiaolan Wang. et al. The total flavonoids from Selaginella tamariscina (beauv.) Spring improve glucose and lipid metabolism in db/db mice. Iran J Basic Med Sci. 2020 Oct; 23(10): 1286C1292Read more>>
  • Cao Zhanhong. et al. Protective Effects of Huangqi Shengmai Yin on Type 1 Diabetes-Induced Cardiomyopathy by Improving Myocardial Lipid Metabolism. Evid-Based Compl Alt. 2021;2021:5590623Read more>>
  • Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Shihe Zhang. et al. Astragalus polysaccharide regulates brown adipocytes differentiation by miR-6911 targeting Prdm16. 2021 Nov 05Read more>>
  • Huimin Wang. et al. Chronic exposure of bisphenol-A impairs cognitive function and disrupts hippocampal insulin signaling pathway in male mice. TOXICOLOGY. 2022 Apr;472:153192Read more>>
  • Yao Li. et al. Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity. INT J BIOL MACROMOL. 2022 Aug;215:113Read more>>
  • Fu-Rong Yang. et al. Xiaoyaosan Exerts Antidepressant-Like Effect by Regulating Autophagy Involves the Expression of GLUT4 in the Mice Hypothalamic Neurons. FRONT PHARMACOL. 2022; 13: 873646Read more>>
  • Zhe Jiang. et al. RSL1D1 modulates cell senescence and proliferation via regulation of PPAR mRNA stability. LIFE SCI. 2022 Aug;:120848Read more>>
  • Cheng Wanpeng. et al. Transcriptomic analysis reveals the effects of maternal selenium deficiency on placental transport, hormone synthesis, and immune response in mice. METALLOMICS. 2022 AugRead more>>
  • Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292Read more>>
  • Afolabi Oladele Ayobami. et al. Diet-induced insulin resistance altered cardiac GLUT4 and FATP/CD36 expression in rats. Beni-Suef University Journal of Basic and Applied Sciences. 2022 Dec;11(1):1-8Read more>>
  • Yuge Jiang. et al. Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake.. FRONT NUTR. 2022 Nov;9:1039753-1039753Read more>>
  • Ying-Ying Yang. et al. The human islet amyloid polypeptide reduces hippocampal tauopathy and behavioral impairments in P301S mice without inducing neurotoxicity or seeding amyloid aggregation. EXP NEUROL. 2023 Apr;362:114346Read more>>
  • Zheng Ran. et al. MicrobiomeCmetabolomics analysis reveals the potential effect of verbascoside in alleviating cognitive impairment in db/db mice. FOOD FUNCT. 2023 MaRead more>>
  • I-Shiung Cheng. et al. Oral post-exercise garlic extract supplementation enhances glycogen replenishment but does not up-regulate mitochondria biogenesis mRNA expression in human-exercised skeletal muscle. J INT SOC SPORT NUTR. 2024 Apr 04Read more>>
  • Cheng Aoming. et al. The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma. CELL DEATH DIS. 2024 May;15(5):1-14Read more>>
  • Giulia Abruscato. et al. Modulation of Glucose Consumption and Uptake in HepG2 Cells by Aqueous Extracts from the Coelomic Fluid of the Edible Holothuria tubulosa Sea Cucumber. BIOLOGY-BASEL. 2024 Jun;13(6):378Read more>>
  • Zhilan Hu. et al. Effects of asiaticoside on the model of gestational diabetes mellitus in HTR-8/svneo cells via PI3K/AKT pathway. J OBSTET GYNAECOL. 2024 24Read more>>
VALIDATION IMAGES

Mouse heart lysates probed with Anti-GLUT4 Polyclonal Antibody (bs-0384R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-GLUT4 Polyclonal Antibody (bs-0384R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


K562 cells probed with GLUT4 Polyclonal Antibody, unconjugated (bs-0384R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)


Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (bs-0384R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Heart lysates; Lane 3: Rat Heart lysates; Lane 4: Mouse Muscle lysates; Lane 5: Rat Muscle lysates probed with GLUT4 Polyclonal Antibody, Unconjugated (bs-0384R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with GLUT4 polyclonal antibody, unconjugated (bs-0384R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.