bs-0297R-HRP
[Conjugated Secondary Antibody]
Rabbit Anti-Human IgG Antibody (H+L), HRP Conjugated
DATASHEET
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Conjugation: HRP
Excitation/ Emission: N/A
Size: 200ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Tan, Yuyu, et al. "Proximity-dependent Protein Detection Based on Enzyme-Assisted Fluorescence Signal Amplification." Biosensors and Bioelectronics (2013).Read more>>
- Wang, Liying, et al. "Sensitivity enhancement of SPR biosensor with silver mirror reaction on the Ag/Au film." Talanta 78.1 (2009): 265-269.Read more>>
- Li, Rui, et al. "4–Amino–1–(3–mercapto–propyl)–pyridine hexafluorophosphate ionic liquid functionalized gold nanoparticles for IgG immunosensing enhancement." Analytical Chemistry (2014).Read more>>
- Li, Rui, et al. "4–Amino–1–(3–mercapto–propyl)–pyridine hexafluorophosphate ionic liquid functionalized gold nanoparticles for IgG immunosensing enhancement." Analytical Chemistry (2014).Read more>>
- Zhang, Si, et al. "A double signal electrochemical Human immunoglobulin G immunosensor based on gold nanoparticles-polydopamine functionalized reduced graphene oxide as a sensor platform and AgNPs/Carbon nanocomposite as signal probe and catalytic substrate." Biosensors and Bioelectronics (2015).Read more>>
- Wang et al. Preparation of colloidal gold immunochromatographic strip for detection of Paragonimiasis skrjabini. (2014) PLoS.One. 9:e92034Read more>>
- Wang,et al.Vasoactive intestinal peptide inhibits airway smooth muscle cell proliferation in a mouse model of asthma via the ERK1/2 signaling pathway.(2018) Experimental Cell Research. 364:168-174.Read more>>
- Wang H et al. CD137 ligand feedback upregulates PD‐L1 expression on lung cancer via T cell production of IFN‐γ. Thorac Cancer. 2019 Oct 17. Read more>>
- Li, Qianlin. et al. Highly potent multivalent VHH antibodies against Chikungunya isolated from an alpaca nave phage display library. J NANOBIOTECHNOL. 2022 Dec;20(1):1-15Read more>>
- Jiahui Guo. et al. Determination of the Predictive Roles and Potentially Pathogenic Antigen Epitopes of α-Enolase Related to the Development of Miscarriage in Females with Autoimmune Thyroiditis. INT J MOL SCI. 2023 Jan;24(2):1021Read more>>
- Aiping Wang. et al. Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV. J VIROL METHODS. 2024 Feb;324:114874Read more>>
- Wenjing Sun. et al. Regulator of Ribosome Synthesis 1 (RRS1) Stabilizes GRP78 and Promotes Breast Cancer Progression. MOLECULES. 2024 Jan;29(5):1051Read more>>