bs-0297G-Biotin [Conjugated Secondary Antibody]
Goat Anti-Human IgG Antibody (H+L), Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Goat

Clonality: Polyclonal

Isotype: IgG

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months..

Background:

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 200ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wu, Lei, et al. "A SERS-based immunoassay with highly increased sensitivity using gold/silver core-shell nanorods." Biosensors and Bioelectronics (2012).Read more>>
  • Tan, Yuyu, et al. "Proximity-dependent Protein Detection Based on Enzyme-Assisted Fluorescence Signal Amplification." Biosensors and Bioelectronics (2013).Read more>>
  • Dong, Peipei, et al. "Ultrathin Gold-Shell Coated Silver Nanoparticles onto a Glass Platform for Improvement of Plasmonic Sensors." ACS Applied Materials & Interfaces (2013).Read more>>
  • Wang, Leilei, et al. "A C1q Domain Containing Protein from Scallop Chlamys farreri Serving as Pattern Recognition Receptor with Heat-Aggregated IgG Binding Activity." PloS one 7.8 (2012): e43289.Read more>>
  • Wang, Leilei, et al. "A C1q Domain Containing Protein from Scallop Chlamys farreri Serving as Pattern Recognition Receptor with Heat-Aggregated IgG Binding Activity." PloS one 7.8 (2012): e43289.Read more>>
  • Hao, Nan, et al. "An electrochemical immunosensing method based on silver nanoparticles." Journal of Electroanalytical Chemistry 656.1 (2011): 50-54.Read more>>
  • Tian, Lifang, et al. ʺAutoimmune and inflammatory responses in Kashin–Beck disease compared with rheumatoid arthritis and osteoarthritis.ʺ Human Immunology 72.10 (2011Read more>>
  • Zhang, Ruohu, et al. "Immunoassays based on surface-enhanced fluorescence using gap-plasmon-tunable Ag bilayer nanoparticle films." Journal of fluorescence 23.1 (2013): 71-77.Read more>>
  • Zhang, Ruohu, et al. "A Straightforward Immunoassay Applicable to a Wide Range of Antibodies Based on Surface Enhanced Fluorescence." Journal of Fluorescence (2013): 1-9.Read more>>
  • Zhang, Ruohu, et al. "Immunoassays based on surface-enhanced fluorescence using gap-plasmon-tunable Ag bilayer nanoparticle films." Journal of fluorescence 23.1 (2013): 71-77.Read more>>
  • Dai, Xiaoyan, et al. "Influence of the physical and chemical properties of magnetic nanoparticles on their performance in a chemiluminescence immunoassay." Clinical Biochemistry (2013).Read more>>
  • Xun, Yiping, et al. "Identification of prohibitin as an antigen in Behcet’s disease." Biochemical and Biophysical Research Communications (2014).Read more>>
  • Gao, Zhan, et al. "An amphioxus gC1q protein binds human IgG and initiates the classical pathway: Implications for a C1q‐mediated complement system in the basal chordate." European Journal of Immunology (2014).Read more>>
  • Zhao, Rui, et al. "Trace level detections of abrin with high SNR piezoresitive cantilever biosensor." Sensors and Actuators B: Chemical (2015).Read more>>
  • Du H, Shi L, Chen P, Yang W, Xun Y, Yang C, et al. (2015) Prohibitin Is Involved in Patients with IgG4 Related Disease. PLoS ONE 10(5): e0125331.Read more>>
  • Fan, Kequan, et al. "A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate." Third International Symposium on Laser Interaction with Matter. International Society for Optics and Photonics, 2015.Read more>>
  • Wu, Lei, et al. "A reusable biosensor chip for SERS-fluorescence dual mode immunoassay." Third International Symposium on Laser Interaction with Matter. International Society for Optics and Photonics, 2015.Read more>>
  • Wang, Chongwen, et al. "Polyethylenimine-interlayered silver-shell magnetic-core microspheres as a multifunctional SERS substrate." Journal of Materials Chemistry C (2015).Read more>>
  • Zhang, Si, et al. "A double signal electrochemical Human immunoglobulin G immunosensor based on gold nanoparticles-polydopamine functionalized reduced graphene oxide as a sensor platform and AgNPs/Carbon nanocomposite as signal probe and catalytic substrate." Biosensors and Bioelectronics (2015).Read more>>
  • Zhou, Peifu, et al. "CHARACTERIZATION OF A TUBERCULOSIS PATIENT'S SERA REACTIVE MYCOBACTERIUM TUBERCULOSIS TRANSCRIPTION FACTOR RV2175C." Journal of Immunoassay and Immunochemistry 35.2 (2014): 173-182.Read more>>
  • Liu L et al. Exosomal cyclophilin A as a novel noninvasive biomarker for Epstein-Barr virus associated nasopharyngeal carcinoma. Cancer Med. 2019 Jun;8(6):3142-3151. Read more>>
  • He Q et al. Spectral-optical-tweezer-assisted fluorescence multiplexing system for QDs-encoded bead-array bioassay. Biosens Bioelectron. 2019 Mar 15;129:107-117.Read more>>
  • Kong LX et al. Lab-on-Tip: Protruding-shaped All-Fiber Plasmonic Microtip Probe Toward In-situ Chem-Bio Detection. Sensors and Actuators B: Chemical,2019 Dec12, 301:127128.Read more>>
  • Chen X et al. Hydrogel-based microbeads for Raman-encoded suspension array using the reversed-phase suspension polymerization method and ultraviolet light curing. Anal Bioanal Chem. 2020 May;412(12):2731-2741. Read more>>
  • He M et al. A Femtoliter On-Demand Droplet Dispensing System Based on Gigahertz Acoustic Resonator.Lab Chip. 2018 Aug 21;18(17):2540-2546.Read more>>
  • Zhou Y et al. Identification of a novel anti-heat shock cognate 71 kDa protein antibody in patients with Kawasaki disease. Molecular Medicine REPORTS 21: 1771-1778, 2020.Read more>>
  • Zhou Y et al. Identification of a novel anti-heat shock cognate 71 kDa protein antibody in patients with Kawasaki disease. Molecular Medicine REPORTS 21: 1771-1778, 2020.Read more>>
  • Zhang Y et al. Low Level Antibodies Against Alpha-Tropomyosin Are Associated With Increased Risk of Coronary Heart Disease. Front Pharmacol. 2020 Feb 27;11:195. Read more>>
  • Zhu D et al. Deubiquitinating enzyme OTUB1 promotes cancer cell immunosuppression via preventing ER-associated degradation of immune checkpoint protein PD-L1Cell Death Differ.2020 Dec 16.Read more>>
  • Tian C et al. Transient receptor potential ankyrin 1 contributes to the lysophosphatidylcholine-induced oxidative stress and cytotoxicity in OLN-93 oligodendrocyte. Cell Stress Chaperones. 2020 Nov;25(6):955-968.Read more>>
  • Yu Jian. et al. Long non?coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR?146b?5p/Robo1 axis. Exp Ther Med. 2021 Apr;21(4):1-13Read more>>
  • Linlin Sheng. et al. Overexpression of FcRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med. 2020 Oct;46(4):1409-1422Read more>>
  • Yuan Tian. et al. A Low Spring Constant Piezoresistive Microcantilever for Biological Reagent Detection. Micromachines-Basel. 2020 Nov;11(11):1001Read more>>
  • Jia-min Yan. et al. Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress. 2021 Nov 06Read more>>
  • Shaoquan Zheng. et al. Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer. Oncoimmunology. 2022;11(1):2020984Read more>>
  • Zou, Xiaopan. et al. ZMIZ2 promotes the development of triple-receptor negative breast cancer. Cancer Cell Int. 2022 Dec;22(1):1-16Read more>>
  • Zheng Yang. et al. The expression of anti-protein disulfide isomerase A3 autoantibody is associated with the increased risk of miscarriage in euthyroid women with thyroid autoimmunity. Int Immunopharmacol. 2022 Mar;104:108507Read more>>
  • Chengnan Tian. et al. CREB1 transcription-activated lncRNA PVT1 promotes cardiac fibrosis via miR-145/HCN1 axis. Int J Cardiol. 2022 JaRead more>>
  • Xiaofei Wang. et al. 35B5 antibody potently neutralizes SARS-CoV-2 Omicron by disrupting the N-glycan switch via a conserved Spike epitope. Cell Host Microbe. 2022 MaRead more>>
  • Wang, Xiaofei. et al. A potent human monoclonal antibody with pan-neutralizing activities directly dislocates S trimer of SARS-CoV-2 through binding both up and down forms of RBD. SIGNAL TRANSDUCT TAR. Signal Transduct Tar. 2022 Apr;7(1):1-13Read more>>
  • Lin Yao. et al. Nasal Spray of Neutralizing Monoclonal Antibody 35B5 Confers Potential Prophylaxis Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Variants of Concern (VOCs): A Small-scale Clinical Trial. CLIN INFECT DIS. 2022 JuRead more>>
  • Yongqiang Sha. et al. Dehydrocorydaline Accelerates Cell Proliferation and Extracellular Matrix Synthesis of TNFα-Treated Human Chondrocytes by Targeting Cox2 through JAK1-STAT3 Signaling Pathway. INT J MOL SCI. 2022 Jan;23(13):7268Read more>>
  • Zhongxiang Tang. et al. Abnormal Gene Expression Regulation Mechanism of Myeloid Cell Nuclear Differentiation Antigen in Lung Adenocarcinoma. BIOLOGY-BASEL. 2022 Jul;11(7):1047Read more>>
  • Zhao Jinfeng. et al. TGF-_1-Mediated Leukocyte Cell-Derived Chemotaxin 2 Is Associated With Liver Fibrosis in Biliary Atresia. FRONT PEDIATR. 2022 Jul;0:1136Read more>>
  • Wang Lina. et al. PLK1 Is a Potential Prognostic Factor Associated with the Tumor Microenvironment in Lung Adenocarcinoma. BIOMED RES INT. 2022;2022:7848771Read more>>
  • Zhuhe Liu. et al. CXCL7 aggravates the pathological manifestations of neuromyelitis optica spectrum disorder by enhancing the inflammatory infiltration of neutrophils, macrophages and microglia. CLIN IMMUNOL. 2022 Sep;:109139Read more>>
  • Liu Lili. et al. Case report: Immunoadsorption therapy for anti-caspr1 antibody-associated nodopathy. FRONT IMMUNOL. 2022 Sep;0:5317Read more>>
  • Tianci Liu. et al. Differential expression of aerobic oxidative metabolism-related proteins in diabetic urinary exosomes.. FRONT ENDOCRINOL. 2022 Sep;13:992827-992827Read more>>
  • Changhao Li. et al. Piezoelectric Bioactive Glasses Composite Promotes Angiogenesis by the Synergistic Effect of Wireless Electrical Stimulation and Active Ions. ADV HEALTHC MATER. 2023 Mar;:2300064Read more>>
  • Xinglei Huang. et al. Downregulation of LncRNA GCLC-1 Promotes Microcystin-LR-Induced Malignant Transformation of Human Liver Cells by Regulating GCLC Expression. TOXICS. 2023 Feb;11(2):162Read more>>