bs-0296G-Biotin
[Conjugated Secondary Antibody]
Goat Anti-Mouse IgG Antibody (H+L), Biotin Conjugated
DATASHEET
Host: Goat
Clonality: Polyclonal
Isotype: IgG
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months..
Background:
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Conjugation: Biotin
Excitation/ Emission: N/A
Size: 200ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Yu, Li-Juan Tang, and Jian-Hui Jiang. ʺSERS-Based, Homogeneous, Multiplexed Immunoassay with Antibody-Fragments Decorated Gold Nanoparticles.ʺ Analytical Read more>>
- An, Yarui, et al. "Sensitive electrochemical immunosensor for ??-synuclein based on dual signal amplification using PAMAM dendrimer-encapsulated Au and enhanced gold nanoparticle labels."Biosensors and Bioelectronics (2011)..Read more>>
- Li, Gang, et al. "Overexpression of human papillomavirus (HPV) type 16 oncoproteins promotes angiogenesis< i> via</i> enhancing HIF-1?? and VEGF expression in non-small cell lung cancer cells."Cancer letters 311.2 (2011): 160-170..Read more>>
- Sun, Yun, et al. "Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection." Infection and immunity 80.8 (2012): 2948-2955.Read more>>
- Zhao, Zhiyi, et al. "Maxadilan prevents apoptosis in iPS cells and shows no effects on the pluripotent state or karyotype." PloS one 7.3 (2012): e33953.Read more>>
- Zhang, Hua, et al. "Novel SPR biosensors based on metal nanoparticles decorated with graphene for immunoassay."?Sensors and Actuators B: Chemical?(2013).Read more>>
- Zhang, Min, Zhi-zhong Xiao, and Li Sun. "Overexpression of NF-??B inhibitor alpha in< i> Cynoglossus semilaevis</i> impairs pathogen-induced immune response."Developmental & Comparative Immunology 36.1 (2012): 253-257..Read more>>
- Zhang, Zi?\ji, et al. "iRNA expression profile during osteogenic differentiation of human adipose?\derived stem cells."Journal of cellular biochemistry 113.3 (2012): 888-898..cRead more>>
- Li, Mo-Fei, et al. "A peptidoglycan recognition protein from< i> Sciaenops ocellatus</i> is a zinc amidase and a bactericide with a substrate range limited to Gram-positive bacteria." Fish & Shellfish Immunology (2011).Read more>>
- Yu, Lan-ping, et al. "Characterization of a c-type lysozyme of Scophthalmus maximus: expression, activity, and antibacterial effect." Fish & Shellfish Immunology (2012).Read more>>
- Yu, Lan-ping, et al. "Immunological study of the outer membrane proteins of< i> Vibrio harveyi</i>: insights that link immunoprotectivity to interference with bacterial infection."?Fish & Shellfish Immunology?(2013).Read more>>
- Li, Mo-fei, et al. "CsTrx80, a truncated form of thioredoxin, possesses chemokine-like property and enhances the immune defense of< i> Cynoglossus semilaevis</i> against bacterial infection." Fish & Shellfish Immunology (2013).Read more>>
- Gao, Qian, et al. "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." Molecular biology reports 39.5 (2012): 5791-5804.Read more>>
- Gao, Fei, and YanFen Ye. "Water bath hyperthermia reduces stemness of colon cancer cells." Clinical Biochemistry (2013).Read more>>
- Wu, Y. H., et al. "A Novel Cytotoxic T lymphocyte Epitope Analogue with Enhanced Activity Derived From Cyclooxygenase?\2."Scandinavian Journal of Immunology 76.3 (2012): 278-285..Read more>>
- Fan, Tingjun, et al. "Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane." Molecular vision 17 (2011): 469.Read more>>
- Fan, Tingjun, et al. "Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane." Molecular vision 17 (2011): 469.Read more>>
- Dong, Wei, et al. "Labeling of human hepatocellular carcinoma cells by hexamethylene diamine modified fluorescent carbon dots." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2013).Read more>>
- Dong, Wei, et al. "Labeling of human hepatocellular carcinoma cells by hexamethylene diamine modified fluorescent carbon dots." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2013).Read more>>
- Sun, Yun, et al. "SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region." Acta Veterinaria Scandinavica 55.1 (2013): 78.Read more>>
- Sun, Yun, et al. "SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region." Acta Veterinaria Scandinavica 55.1 (2013): 78.Read more>>
- Feng, Yongqiang, et al. "Combination of ketamine and xylazine exacerbates cardiac dysfunction in severely scalded rats during the shock stage." Experimental and Therapeutic Medicine (2013).Read more>>
- Sun, Na, et al. "Antiviral activity and underlying molecular mechanisms of Matrine against porcine reproductive and respiratory syndrome virus in vitro." Research in Veterinary Science (2013).Read more>>
- Fu, Ying, et al. Read more>>
- Xu, R., et al. "Secretoneurin Induces Airway Mucus Hypersecretion by Enhancing the Binding of EGF to NRP1." Cellular Physiology and Biochemistry 33.2 (2014): 446-456.Read more>>
- Xu, R., et al. "Secretoneurin Induces Airway Mucus Hypersecretion by Enhancing the Binding of EGF to NRP1." Cellular Physiology and Biochemistry 33.2 (2014): 446-456.Read more>>
- Zeng, Q. J., et al. "Decorin-induced proliferation of avian myoblasts involves the myostatin/Smad signaling pathway." Poultry Science 93.1 (2014): 138-146.Read more>>
- Liu, Qun, et al. "6-Shogaol induces apoptosis in human leukemia cells through a process involving caspase-mediated cleavage of eIF2α." Molecular Cancer 12.1 (2013): 135.Read more>>
- Gong, Tao, et al. "The Control of Mesenchymal Stem Cell Differentiation Using Dynamically Tunable Surface Microgrooves." Advanced Healthcare Materials (2014).Read more>>
- Lu, Shuai, et al. "Proteoglycans affect monomer infiltration in the etch-and-rinse bonding technique." Dental Materials (2014).Read more>>
- Li, Yansong, et al. "A monoclonal antibody based enzyme-linked immunosorbent assay for detection of Phenylethanolamine A in tissue of swine." Food Chemistry (2014).Read more>>
- Geng, Ping, et al. "Amplified Electrochemical Immunosensor for Calmodulin Detection Based on Gold‐Silver‐Graphene Hybrid Nanomaterials and Enhanced Gold Nanorods Labels." Electroanalysis (2014).Read more>>
- Qiao, Hui, et al. "Progressive alterations of hippocampal CA3-CA1 synapses in an animal model of depression." Behavioural Brain Research (2014).Read more>>
- Cao, Jing, et al. "Intrathecal injection of fluorocitric acid inhibits the activation of glial cells causing reduced mirror pain in rats." BMC Anesthesiology 14.1 (2014): 119.Read more>>
- Nie, Chenyao, et al. "Multifunctional Assembly of Micrometer‐Sized Colloids for Cell Sorting." Small (2015).Read more>>
- Zhou, Ze-jun, and Li Sun. "CsCTL1, a teleost c-type lectin that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved EPN motif." Developmental & Comparative Immunology (2015).Read more>>
- Diao, Jin-Mei, et al. "Construction of a human corneal stromal equivalent with non-transfected human corneal stromal cells and acellular porcine corneal stromata." Experimental Eye Research (2015).Read more>>
- Mu, Xihui, et al. "Nano-Magnetic Immunosensor Based on Staphylococcus Protein A and the Amplification Effect of HRP-Conjugated Phage Antibody." Sensors 15.2 (2015): 3896-3910.Read more>>
- Zhang, Bao-cun, and Li Sun. "Tongue sole (Cynoglossus semilaevis) prothymosin alpha: cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and–independent pathways respectively." Developmental & Comparative Immunology (2015).Read more>>
- Hu, Dongfang, et al. "Outbreak of variant pseudorabies virus in Bartha-K61–vaccinated piglets in central Shandong Province, China." Journal of Veterinary Diagnostic Investigation (2015): 1040638715593599.Read more>>
- Sun, Qing-lei, and Li Sun. "A short-type peptidoglycan recognition protein from tongue sole (Cynoglossus semilaevis) promotes phagocytosis and defense against bacterial infection." Fish & Shellfish Immunology (2015).Read more>>
- Sun, Yuan-yuan, Li Liu, and Jun Li. "Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria." Developmental & Comparative Immunology (2015).Read more>>
- Guan, Shan, et al. "An in vitro investigation of a detachable fork-like structure as efficient nuclear-targeted sub-unit in A2780 cell cultures."International Journal of Pharmaceutics (2016).Read more>>
- Li, Lian, et al. "Time-staggered delivery of docetaxel and H1-S6A, F8A peptide for sequential dual-strike chemotherapy through tumor priming and nuclear targeting." Journal of Controlled Release (2016).Read more>>
- Ji, Xianliang, et al. "Intranasal Immunization with Influenza Virus-Like Particles Containing Membrane-Anchored Cholera Toxin B or Ricin Toxin B Enhances Adaptive Immune Responses and Protection against an Antigenically Distinct Virus." Viruses 8.4 (2016): 115.Read more>>
- Guo, Yidi, et al. "GATA2 regulates GATA1 expression through LSD1-mediated histone modification." Am J Transl Res 8.5 (2016): 2265-2274.Read more>>
- Yang, Ting, and Jiang Jiang. "Embedding Raman Tags between Au Nanostar@ Nanoshell for Multiplex Immunosensing." Small (2016).Read more>>
- Hou, Shuai, et al. "Mechanism of Mitochondrial Connexin43′ s Protection of the Neurovascular Unit under Acute Cerebral Ischemia-Reperfusion Injury." International Journal of Molecular Sciences 17.5 (2016): 679Read more>>
- Kim, Daniel, et al. "Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus)." Fish & Shellfish Immunology (2016).Read more>>
- Xia, Dan, et al. "MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation, and PIK3CA-AKT-MTOR signaling."Autophagy (2016).Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:2000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:2000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:2000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-Bio 1:500) instructionsand DAB staining.